Identification of Candida Species Isolated From Oral Colonization in Iranian HIV-Positive Patients, by PCR-RFLP Method.

AUTHORS

Seyyed Amin Ayatollahi Mousavi 1 , * , Samira Salari 2 , Sasan Rezaie 3 , Naser Shahabi Nejad 4 , Sanaz Hadizadeh 4 , Hossein Kamyabi 4 , Hossein Aghasi 4

1 Department of Medical Mycology and Parasitology, School of Medicine, Medical University of Kerman, [email protected], IR Iran

2 Department of Medical Mycology and Parasitology, School of Medicine, Medical University of Kerman, Kerman, IR Iran, and Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Iran

3 Department of Medical Mycology and Parasitology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, IR Iran

4 Kerman Research Center of Infectious and Tropical Medicine, IR Iran

How to Cite: Mousavi S, Salari S, Rezaie S, Nejad N, Hadizadeh S, et al. Identification of Candida Species Isolated From Oral Colonization in Iranian HIV-Positive Patients, by PCR-RFLP Method., Jundishapur J Microbiol. Online ahead of Print ; 5(1):336-340. doi: 10.5812/kowsar.20083645.2429.

ARTICLE INFORMATION

Jundishapur Journal of Microbiology: 5 (1); 336-340
Article Type: Research Article
Received: March 1, 2011
Accepted: May 10, 2011
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Abstract

Background: The incidence of opportunistic infections due to Candida albicans and other Candida spp. has been increasing. Rapid identification of candidiasis is important for the clinical management of immunocompromised patients. Polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) is a rapid, sensitive, and specific method for detection of clinically important fungi.
Objectives: The purpose of this study was to identify Candida spp. isolated from the oral cavities of HIV-infected patients in southeastern Iran (Kerman), by using PCR-based restriction enzyme digestion.
Patients and Methods: We identified 96 Candida isolates obtained from 139 Iranian patients infected with the human  immunodeficiency virus (HIV), between April 2009 and April 2010, by using PCR-RFLP assay. Universal primers for the internal transcribed spacer (ITS) region (ITS1–ITS4) of the fungal rRNA genes were used for this assay.
Results: We successfully identified the different Candida spp. by using the restriction enzyme MspI. C. albicans was the most commonly identified species (82.2%), followed by C. glabrata (7.29%), C. parapsilosis and C. kefyr (both 4.1%), and C. tropicalis (2%).
Conclusions: PCR-RFLP is a highly sensitive, specific, and direct method for fungal detection and can be used for fungal epidemiological studies in HIV-positive and other immunocompromised patients.


  • Implication for health policy/practice/research/medical education:
    To have knowledge about the HIV positive patients and the role of Candida spp. and candidiasis in that group was so important forthe authors.
  • Please cite this paper as:
    Ayatollahi Mousavi SA, Salari S, Rezaie S, Shahabi Nejad N, Hadizadeh S, Kamyabi H. et al. Identification of Candida Species Isolated From Oral Colonization in Iranian HIV-Positive Patients, by PCR-RFLP Method. Jundishapur J Microbiol. 2012;5(1):336-40.
  • DOI:10.5812/kowsar.20083645.2429

© 2012, AJUMS. Published by Kowsar M.P.Co. All rights reserved.


Keywords

Candida spp PCR-RFLP HIV-positive patients Candida albicans

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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