Inhibition and Disruption Properties of Chlorhexidine Gluconate on Single and Multispecies Oral Biofilms

AUTHORS

Gulhas Solmaz 1 , May Korachi 1 , *

1 Genetics and Bioengineering Department, Faculty of Engineering and Architecture, Yeditepe University Kayisdagi, [email protected], Turkey

How to Cite: Solmaz G, Korachi M. Inhibition and Disruption Properties of Chlorhexidine Gluconate on Single and Multispecies Oral Biofilms, Jundishapur J Microbiol. Online ahead of Print ; 6(1):61-66. doi: 10.5812/jjm.4852.

ARTICLE INFORMATION

Jundishapur Journal of Microbiology: 6 (1); 61-66
Published Online: November 1, 2012
Article Type: Research Article
Received: March 19, 2012
Accepted: May 15, 2012
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Abstract

Background: Chlorhexidine gluconate (CHX) is the most common antimicrobial agent used against oral pathogens, however information on its ability to inhibit and disrupt anaerobic single and multispecies biofilms is relatively unknown.

Objectives: The purpose of this study was to test the efficacy of CHX for its biofilm inhibition and disruption properties using crystal violet assay.

Materials and Methods: Biofilm assays were carried out on single and multispecies of four oral pathogens: Streptococcus (S.) mutans, Fusobacterium (F.) nucleatum, Aggregatibacter (A.) actinomycetemcomitans and Porphyromonas (P.) gingivalis.

Results: Streptococcus mutans, Fusobacterium nucleatum and multi-species biofilm formation were inhibited in more than 90% of cases at concentrations of 3-12 mg/L. CHX exhibited strong disruptive activity (> 65%) on one-day old biofilms of A. actinomycetemcomitans and P. gingivalis.

Conclusions: In conclusion, CHX was a high effective biofilm inhibitor on S. mutans, F. nucleatum and multispecies biofilms but had a minimal effect on P. gingivalis and A. actinomycetemcomitans. Conversely, CHX showed disruptive properties on late colonizers in single species biofilms but not on early colonizers and multispecies biofilms.

Keywords

Biofilms Anti-microbial Agent Chlorhexidine Gluconate Periodontitis

© 2012, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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