Insilico Phylogenetic Analysis and Docking Studies of Hexose Transporter in Plasmodium Falciparum

Abstract

In silico analysis, it helps in collecting biological data, identification of new genes and also used for studying evolutionary relationships and functional mechanism. (Jaco De Rider, 2006). CONSURF tool was used to analysis the phylogenetic tree for the protein 6M20 (Plasmodium falciparum Hexose transporter). This information can be used for the identification of a genomic sequence tag, matching the glucose transporter family using BLAST tool (Rina H, Gunawan P.W. 2012). 6M20 is a transporter protein that act as a glucose carrier to the parasite for its growth and development. In this silico study, the active compounds of PfHT1 are screened, which is responsible for glucose transportation and to inhibit it using docking study. HIV protease inhibitors, cancer inhibitors, neem inhibitors and various other malaria inhibitors were used as ligands for the docking analysis. The structure was obtained from PubChem and docking analysis was done using autodock vina tool. Each ligands affinity towards the protein was studied and compared. 6M20 is actually conserved among most other species so, even if the parasite mutates, this transporter protein remains the same.