Phytochemical and Pharmacological Investigation of apium Graveolens

Abstract

Celery (Apium graveolens) is a marshland plant in the family Apiaceae that has been cultivated as a vegetable since antiquity. The present work titled as “Phytochemical and Pharmacological Investigation of Apium graveolens” was undertaken with the aims to study the various quality parameters of Apium graveolens seed as per WHO guidelines and also to study the antioxidant activities. The drug was evaluated to check various quality parameters such as foreign matter analysis, ash values (total ash, water soluble and acid insoluble), extractive values (Hot and cold, extractive values of Petroleum ether, Ethyl acetate, Alcoholic, respectively), moisture content, fat content, resin content, Determination of pH values, Fluorescence analysis, phytochemical screening, phytochemical investigation. Further HPTLC analysis was performed and quantification of drug was done by using quercetin and kaempferol as standard simultaneously by HPTLC. The results were found to be comparable to the standards. The potential of drug as antioxidant agent was evaluated by DPPH free radical scavenging activity using butylated hydroxyl toluene (BHT), butylated hydroxyl anisole (BHA), Vitamin C as standards. IC50 values (The concentration of drug required to scavenge 50 % of DPPH radical) were calculated for sample and standards. IC50values were found to be comparable to that of standards like BHT, Vitamin C and BHA. Further the drug may be evaluated to study the role of quercetin and kaempferol in the treatment of obesity, diabetes, related diabetic disorders and other disease related to metabolic disorders.