“Antiviral potential of leaf, bark and fruit methanol extracts of aegle tamilnadensis abdul kader against influenza a (h1n1) pdmn2009”

Abstract

The methanolic extract of leaf, fruit and bark of the Aegle tamilnadensis have been prepared and characterized by using spectral studies, such as UV-Visible, FT-IR and GC-MS techniques. Then, the three methanolic extracts have been screened for their antiviral properties from the MDCK cell line in the Influenza A (H1N1) pdmn2009 samples. TCID₅₀ has been measured. The cytotoxicity assay CC₅₀ was also observed for its leaf, bark and fruit extract. Influenza A (H1N1) pdmn2009 virus in the MDCK cell lines was detected in the preliminary antiviral activity of the leaf, fruit and bark methanolic extracts. Among three methanolic extracts, the fruit extract has been shown better activity than leaf and bark extracts. It may be enhanced antiviral efficacy due to the presence of some bioactive compounds such as, Phenol derivatives, 7-Hydroxycoumarin, 4,7,8-trimethoxyfuro[2,3-b]quinolone, (E)-7-(3,7-dimethylocta-2,6-dienyloxy)-2H-chromen-2-one, 3β-Trimethylsiloxy-5α,6α-epoxycholestane, Epoxyaurapten, 3',8,8'-Trimethoxy-3-piperidyl-2,2'-binaphthalene-1,1',4,4'-tetrone etc., in the methanolic fruit extract