Use the Alteration of Serum Omentin-1 as Predictors for Nonalcoholic Fatty Liver Disease

Abstract

Background:Non-alcoholic fatty liver disease (NAFLD) is an accumulation of triglycerides in the cells of liver without inflammation or damage to cells  of liver in people  who don’t drink alcohol. The current study aimed to estimation of Omentin-1, insulin,Aspartate aminotransferase(AST), Alanine aminotransferase(ALT), Alkaline phosphatase(ALK),random blood sugar (RBS), Albumin (ALB), Total Protein, globulin, triglycerides(TG), totalcholesterol(TC), high-density lipoprotein (HDL), low-density lipoprotein(LDL), very low-density lipoprotein(VLDL) in the blood of patients suffering fromNonalcoholic Fatty Liver,  and detecting the alteration of the ratio in the omentin-1 and other biochemical parameters in patients and comparing it with the healthy ones.

Methods: Sixty patients with NAFLD recruited in Al-Muqudadia General Hospital/Diyala Governorate- Iraq for the period1/10/2021 - 30/12/2021. Thirty  healthy individuals were volunteer as control group. The ELISA Kit was used to measure serum omentin-1.A rapid quantitative assayHS-INSULIN-CHECK-1 is used to detection of insulin in serum.AST, ALT, ALK, Albumin, Globulinand Total Protein were measured by use commercial available laboratory kits .The serum RBS, TG, TC, and HDLtests were assessed using an automated chemical analyzer COBS 411.

Results: The levels of serum omentin-1  inNAFLD patients was significantly higher than that of the healthy subjects (p<0.05). There were a significant elevation(p<0.05) inmean value of ALT and AST in patients(27.91±12.88 and 31.13±10.85) in comparison to controls (18.05±5.72 and 23.05±6.80) respectively. The ALK and albumin levels were increased nonsignficantly in patients compared to controls (p>0.05). The results of total protein, RBS , insulinandglobulin, showed non-significant elevationin patients compared to healthy group (p>0.05). There were substantial significant elevation(p<0.05) of serum TC, TG, LDL, and VLDL, in patients in comparison to control group while HDL levels were non significantly decreased. The concentration of serum omentin-1, on the other hand, had higherarea under curve(AUC)and sensitivity in ROC curve in comparison to otherbiochemical parameters.

Conclusion:Omentin-1 levels were significantly elevated in patients of NAFLD and had higherAUC andsensitivity in ROC curve. Therefore, theincreasing of serum omentin-1(with increaseserum TC, TG, LDL, VLDLand liver functions parameters)may be a positive predictor in patientsof non-alcoholic fatty liver disease.