A Phenotypic Study of the Resistance of Bacteria Klebsiella Pneumonia to Some Antibiotics and the Study of Its Dominant Pattern

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Areej Abdul Redha Makki, Azhar Noory Hussein


200 samples were collected from patients suffering from different infections and different age groups for both sexes and from different sources distributed on 80 (urine) samples , 42 (stool) samples , 62 samples (Sputum) and 26 samples from (burns) and (wounds) from patients in Al-Diwaniyah Governorate hospitals, whose ages range between 1-60 years and for a period of four months, from 9/15/2021 to 01/20/2022. Samples were planted on culture media and isolates were diagnosed using traditional methods (culture, microscopic and biochemical).

The vitek2 device was used to confirm the diagnosis of K. pneumoniae isolates, and the confirmation was reinforced by using the molecular diagnosis of the SrRNA 16 gene of K. pneumoniae, and the diagnostic results showed a return of 36 K. pneumoniae isolates with a percentage of 22.1%.

The results of the antibiotic susceptibility test showed that isolates of K. pneumoniae bacteria were resistant to beta-lactams represented by Ampicillin and Amoxicillin, which were 100% and 94%, respectively, while they were resistant to cephalospores belonging to the same group, represented by Cefotaxime and Ceftazidime, by 72.2% and 55.5%, respectively. As for its resistance to the aminoglycosides represented by Amikacin, it was 22.2%. As for the fluoroquinolones, which included the anti-levofloxacin and Ciprofloxacin, their resistance was equal, as it was 27.7%. K. pneumoniae isolates showed a resistance to Trimethoprim antibody amounted to 66.6%, and the isolates showed resistance to Chloramphenicol antibody by 33.3%.

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