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Nosocomial infections or Hospital acquired infections are a group of infections mostly seen among sick group and are usually caused by intrusive equipment and non-sterile procedures. UTI stands to be the one of the most commonly encountered nosocomial infection and is mostly caused by certain bacteria and a broad variety of fungi. Candida spp are commensal organisms and can cause opportunistic infections by colonizing on mucosal surfaces. The isolation of candida species in urine leads to a wide range of interpretations, which poses a challenge to clinicians to determine whether candiduria is due to contamination or infection in the upper or lower respiratory tract. Hence, this current study aimed in identify the predominant Non-candida albicans isolated from urine specimens, to differentiate their biochemical characteristics, and to determine their Antifungal susceptibility testing by disc diffusion as recommended by CLSI.