Cytotoxicity of Iraqi Ethanolicpropolis Extract on Human Colon Cancer Cell Line (SW480)

Abstract

The aim of the study is to try to find natural alternatives used in the treatment of cancerous diseases. The relationship between the secondary metabolism product of propolis metabolism and the most dangerous disease that affects humans, which is cancer, was studied using the colon cancer cell line (SW480) in humans. This study includes testing the possibility of the toxic effect of Iraqi ethanolicPropolisextract against the human colon cancer cell line and trying to find natural alternatives used in the treatment of cancerous diseases by using six sequential concentrations of the ethanolic extract (1000, 500, 250, 125, 62.5, 31.25) mg/ml for a period of time from Exposure (24) hours at the level of significance (p < 0.05). The chemical detection of the compounds present in the crude propolis was done by HPLC teast, as it was found that the raw propolis contains (phenol, flavone, glycoside, alkaloid, tannin, saponins). The cytotoxic effect of the extract was investigated, as the results showed that Iraqi ethanolicPropolisextract has severe cytotoxicity that increases with increasing concentration through an increase in growth inhibition rates and a decrease in the number of cancer cells.