Detection of Intrleukin-17 and Tumor Necrosis Factor TNF-Α Level in Iraqi Patients with Relapsing‑remitting Multiple Sclerosis

Abstract

Background:Multiple sclerosis (MS) is caused by immune cells attacking and destroying the insulation that keeps neuronal signals traveling in the brain, triggering a chemical cascade. These chemicals, known as cytokines, cause inflammation in the brain, which attracts additional immune cells and leads to the severe condition of neurological function loss. A key cytokine's role in multiple sclerosis has been uncovered, and it could be a target for novel therapies. The interleukin-17/tumor necrosis factor (TNF-α) axis has a role in the pathophysiology of autoimmune diseases. The interleukin-17/tumor necrosis factor (TNF-α) axis is involved in the pathophysiology of a number of autoimmune diseases.The goal of this study was to detection the levels of IL-17 and TNF- in patients with multiple sclerosis (MS) and its correlatin with gender.

Objective:To assess the concentration of IL-17 and TNF-α in RRMS patients and its correlation with gender by  usingELISA technique.

Methods:The current study comprised fifty patients with relapse remitting MS. Blood was drawn from 50 MS patients and 40 healthy people who served as controls. The serum levels of IL-17 and TNF- were determined using an enzyme-linked immunosorbent assay (ELISA).

Results: Our results indicate that concentrations of IL-17and TNFα cytokines were also significantly higher in patients with RRMS compared with healthy individuals (P< 0.000006 and P< 2.27 x 10⁻¹², respectively). The male with MS had higher IL-17 and TNF-α concentrationsthan female patients (P< 0.050).

Conclusion:The current study's findings demonstrated a significant rise in serum levels of IL-17 and TNF-α in MS patients when compared to healthy controls, which could be influenced by gender.