Comparative Study of Total Phenolic Content in Curcuma Longa Mother Tinctures from Different homoeopathic Pharmaceutical Companies.

Abstract

Background:

Total Phenolic Content is the process to fraternize the amount of phenolic content in the samples. It was ascertained by using Folin-Ciocalteu reagent. Curcuma longa the “Golden Spice” is a rhizomatous, perennial, herbaceous plant belonging to Zingiberaceae family. ELISA MICROPLATE READER is an basic apparatus used to encounter specific molecules. 96 well plate is utilized for the sample scrutiny.

Introduction:

Curcuma longa the “Golden Spice” is a rhizomatous, perennial, herbaceous plant belonging to Zingiberaceae family.¹⁰ It was first discovered about two centuries ago when Vogel and Pelletier reported by isolating a “yellow colouring matter” from rhizomes of Curcuma longa. It extends up to 1 m high with a short stem, aromatic rhizomes hermaphrodite flowers scatter throughout tropical and subtropical regions of the world.Total Phenolic Content is the process to fraternize the amount of phenolic content in the samples. It was ascertained by using Folin-Ciocalteu reagent. The principle of the F–C assay is the depletion of the FCR in the presence of phenolic ensue in the proffering of molybdenum–tungsten blue color that was gauged spectrophotometrically at 760 nm

Method:

1ml of Curcuma longa mother tincture from various homoeopathic pharmaceutical companies was amalgamated  with 1ml of Gallic acid solution, 5ml of Folin-Ciocalteu Reagent Solution and Sodium Carbonate Solution in different test tubes as (A,B,C,D,E,G,H,I).

Results:

Phenolic absorbance was quantified by using Elisa micro plate reader at 500 micro grams of Gallic acid / ml at 660nm.Highest amount of phenols was esteemed in test tube c i.e Curcuma longa mother tincture prepared from LORDS Homoeopathic Pharmaceutical Company

Conclusion:

The Total phenolic content in Curcuma longa mother tinctures inclined from different homoeopathic pharmaceutical companies was ascertained by accomplishing the experiment. Phenolic absorbance was quantified by using Elisa micro plate reader at 500 micro grams of Gallic acid / ml at 660nm. The results gained from the present study had flaunted that sample C i.e. Curcuma longa mother tincture groomed from the LORDS homoeopathic pharmaceutical company exhibited highest amount of phenols than other Homoeopathic pharmaceutical companies.