Standardization, Invitro- Antioxidant Activity and Neuroprotective Effects in the Roots of Berberis Tinctoria Lesch

Abstract

Objective : To perform  the Standardization, invitro- antioxidant activity and neuroprotective effects in  different extracts of Berberis tinctoria root.

Methods – Qualitative and quantitative analysis was performed for various extracts of Berberis tinctoria, quantitative investigation was done by total phenolic content, total flavonoid and total alkaloidal content. The invitro antioxidant were performed by using DPPH ( 1,1 diphenyl-2-picryl hydrazyl ) , Hydroxyl radical scavenging activity, Nitric Oxide scavenging activity and superoxide radical scavenging activity . Statical analysis were done by prism graph pad version 9.Finger print HPTLC investigation was performed to determine the major phytoconstituents in the ethanolic fraction of Berberis tinctoria extracts. The cell morphology and cell viability of Berberis tinctoria ethanol fraction, were determined by using neuroblastoma cells MTT assay methods.

Results and discussion – Phytochemical assessment exhibit the the presence of Carbohydrate, Alkaloids, Flavonoids, Phenol, Saponins, Tannins and Cardiac Glycosides in acetone, chloroform extracts and ethanolic fraction . Quantitative analysis shows the presence of alkaloids , phenolic and flavonoid content in ethanolic and chloroform fraction of Berberis tinctoria roots. . Anti-oxidant activity reveals that BTEF ( Berberis tinctoria  Ethanolic fraction ) and BTCF ( Berberis tinctoria chloroform fraction ) having higher percentage of inhibition fraction. And IC 50 values gives a strong justification to this data. HPTLC fingerprint analysis exhibited the presence of berberine at 254 nm. Invitro neuroprotective cell line studies justify that Berberis tinctoria having a better neuroprotective activity. 

Conclusion: The findings gives appropriate evidence to recommend the usage of Berberis tinctoria roots  in scavenging reactive oxygen species and to protect the cells from toxic events. And the berberine present in the ethanolic fraction shows its potentiality in neurological disorders. Ethanolic fraction of Berberis tinctoria having potential neuroprotective activity to overcome the neural toxicity that leads to behavioural and various mood disorders in brain.