Streptomyces sp Isolated from Vermicasts: Statistical Optimization of Culture Conditions for the Production of Bioactive Compounds

Abstract

In the present work, the culture conditions appropriate for the maximum production of bioactive metabolites by Streptomyces spp. isolated from vermicasts were tested. Two of the Streptomyces sp. isolated from vermicasts collected from Madhavaram Botanical Garden and Agriculture farm from Thanjavur were subjected to various environmental optimization conditions. These isolates were subjected to various physicochemical parameters and the optimum conditions were identified for the maximum production of bioactive compounds with antimicrobial activity. US1 showed a maximum biomass production in Starch casein broth medium with a higher zone of inhibition against Bacillus subtilis; whereas, US3 showed a maximum biomass production and bioactive compound production in nutrient broth. Both isolates US1 and US3 showed maximum cell growth and zone of inhibition in the medium containing starch as a carbon source. For US1, maximum growth and zone of inhibition were observed in the medium supplemented with casein as a nitrogen source.