Molecular Genetic Testing of Sheep by Gene (PRL) and (CSN1S)1

Abstract

Introduction. The goal of modern breeding approaches is to uncover molecular genetic markers that interact with economically valuable features. Polymorphism, which is a change in the nucleotide sequence in a DNA molecule produced by numerous mutations, is one of the most important properties of markers. The allelic spectrum is one of its manifestations. For identifying allelic variation in candidate genes, the PCR-RFLP technique is regarded the gold standard. Prolactin (PRL) and -casein are two interesting genes that have been proposed as sheep productivity markers (CSN1S).

Methods. The perchlorate method, polymerase chain reaction (PCR), DNA restriction analysis (PFLP), and gel electrophoresis methods were used to screen sheep for the (PRL) and (CSN1S)1 genes.

Results. The polymorphism of the allelic spectrum of PRL genes in Texel, Prekos, and Karakul sheep was identified as a result of the investigation using the PCR-PFPL method.

The information gathered can be used as a genetic feature of this breed's sheep population, as well as in breeding and pedigree work aimed at maintaining genetic variety.

Conclusion. The study discovered that the AA genotype has an effect on sheep milk productivity, with this genotype being more productive than other genotypes.

The information gathered can be used to assess the state of the breed gene pool and forecast productivity.