Assessment of MMP-2 and IL-35 Role in Threatened Abortion

Abstract

Introduction:Threatened abortion is pregnancy-related bloody vaginal discharge or frank bleeding in the first half of pregnancy that does not result in cervical dilation. The present study aimed to assessment the serum levels of Matrix Metaloproteinase-2 and IL-35, as well as their role in threatened abortion.

Methods:The research was conducted in the Iraqi city of Babylon. The 90 participants in this study were categorized into 3 groups: 30 threatened abortion patients, 30 healthy pregnant women, as well as 30 healthy non-pregnant women. The study groups were all made up of people between the ages of 20 and 35 years.Assessment of MMP-2 and IL-35serum levels in the studied groups was made using the Enzyme linked Immunosorbent Assay (ELISA) technology.

Results:MMP2 level was highly significant elevated (326.42 Pg/ml) among the pregnant women sera in comparison with threatened abortion group (282.27 Pg/ml) and non-pregnant women (235.25 Pg/ml)  (P= 0.001), on other hand, the mean of IL-35 level was highly significant elevated (363.95 Pg/ml) among the pregnant women sera in comparison with threatened abortion group (244.05 Pg/ml) and non-pregnant women (187.82 Pg/ml)  (P= 0.001).

Conclusion:It is concluded that elevated MMP-2 serum levels in pregnant women compared with non-pregnant women, while a non-significant difference was obtained between  threatened abortion group and pregnant women group, while IL-35 levels were significantly decreased in TA patients' sera.