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Our current research includes the inhibitory ability of copper oxide nanoparticles to inhibit the growth of bacteria E.coli, as an antibacterial, and to document the appropriate optimum concentration in performing the inhibitory ability of zinc oxide nanoparticles against E.coli.The effectiveness of zinc nanoparticles on E.coli colonies grown on Petri-dishes was shown, and the concentrations and percentages tested for this purpose are (10%, 25%, 50%, 75%, 100%), respectively. The minimum concentration of zinc oxide nanoparticles that suitable as an effective antibacterial in inhibiting the growth of E.coli bacteria is (50%), at which it becomes clear that bacteria will not grow on the plate within a certain area. The inhibitory ability increases as the concentration of zinc nanoparticles increases until it reaches to an percentage of (100%), and this concentration is considered the ideal concentration, while the comparison is made with the antibiotic cefotaxime, which is the antibiotic effective in inhibiting the growth of E.coli bacteria.It is clear from the work that the area of the retarding area is (20 m.m). Therefore, through this research work, we can consider copper oxide nanoparticles as a suitable antibiotic, and an effective treatment for all infections and types of diseases that are caused by E.coli, as it is parallel in the inhibitory activity of the antibiotic cefotaxime (CX30). It gave the same result and the inhibitory ability to prevent the growth of this type of bacteria.