Sequencing Analysis of eIF4AII in Bursa of Fabricius Infected with Infectious Bursal Disease Virus

Abstract

Eukaryotic initiation factor 4AII (eIF4AII) genes play a key role in achieving the translation of both capped and uncapped mRNAs. Its involvement with the Infectious Bursal Disease Virus (IBDV) suggests an active viral protein (VP1) replication. This study aimed to analyze eIF4AII of bursa of Fabricius in locally infected and non-infected chicken with IBDV. Fifty samples of bursa of Fabricous were collected from Gallus gallus from different areas in Iraq. Samples were subjected for RNA and DNA extraction and conventional PCR for the amplification of the VP1 of IBDV and eIF4AII gene in gallus gallus. The results of sequencing analysis of eIF4AII in field samples non-infected Bursa of Fabricius revealed that the gene is highly conserved among with silent mutation. Nevertheless, the sequencing analysis of eIF4AII in field samples infected Bursa of Fabricius revealed that the gene is highly conserved among with missense mutation at several location. The phylogenetic analysis revealed that the eIF4AII of Iraqi isolates was related to the USA and Germany strains at 99% similarity. Phylogenetic analysis revealed that the eIF4AII gene of Bursa of Fabricius obtained from infected and non-infected chickens were classified as a Gallus gallus eIF4AII. This confirmed that the eIF4AII gene in Gallus gallus is highly conserved in IBDV infected and non-infected chicken.