Detection of Several Virulence Gen in E. Coli Isolated from Beef and Broiler Chicken in Iraq

Abstract

Background: The antibiotic resistance of bacteria which contaminate food products is considered as one of the biggest public health concerns in all over the world.

Objectives :  In this study we examine the detection Of the virulence gen of Escherichia coli isolated from minced beef, chicken meat, milk samples and urinary tract infection in Baghdad, Iraq.

Methods : A total of 60 samples of minced beef meat, slaughtered chicken meat and milk samples had been collected from meat and milk markets in Baghdad city, Iraq, while the urinary tract infection samples were collected from the patient in medical city hospital.  After microbiological culture, the isolates were identifying by biochemical tests. Virulence gen of E. coli isolates were determining by conventional PCR.

Results : Out of 60 samples, 15 samples (25%) were positive to E. coli which were identified and confirmed by cultural and biochemical tests. The results of conventional PCR product which was doing to the fifteen samples were as the following, the aea and stx1 was found in all samples (100%), LT was found in (80%) and stx2 (75%). In this study, we aimed to detect the stx1, stx2, LT and eae virulence gen which responsible for multidrug resistance in E. coli isolates which may be due to the heavy usage of these drugs in poultry and domestic animals in Iraq.

Conclusion : In this study we detect the virulence gen of Escherichia coli isolated from minced beef, chickens meat, milk samples and urinary tract infection in Baghdad, Iraq and that may explain The overuse of antibiotics for treatment of the animal's diseases could play the main role in spreading of the antibiotic resistance genes among pathogenic bacteria.