Main Article Content
The study included 100 samples of atherosclerosis serum from healthy and sick people and an assessment of the effectiveness of monoamine oxide enzyme and an assessment of the effectiveness of heart enzymes such as creatine kinez enzyme and lactate dehydrogenase enzyme and fat levels; (cholesterol, triglyceride, high-density lipid protein density (HDL-c), low-density lipoproteins (LDL-c), very low-density lipoproteins (VLDL-c)). Estimating levels of oxidative stress, antioxidants and other biochemical parameters, there was also an aspect of research that involved partial purification of the enzyme from the serum of atherosclerosis patients, estimation of its molecular weight and the motor properties of the enzyme also synthesis for Fe3O4 nanoparticles were made. Results showed that there was a moral increase (P≤0.0001) in the levels of the enzyme MAO serum of atherosclerosis patients compared to healthy, there was also a moral increase (P≤0.0001) In cholesterol, triglycerides, LDL, VLDL the results also showed a high level of MDA and, NOO and GSHD patients had a moral rise at P < 0.001 probability level) Compared with health, results showed a moral rise in the efficacy of CK in the serum of atherosclerosis patients at a probability level (P ≤ 0.001) at 183.9400 ± 18.4008 compared to healthy 147.5800 ± 13.7382. Also, results showed an unmorable rise in LDH in the serum of atherosclerosis patients at a probability level P = 0.1485: 30.8200 ± 2.1447 compared to 30.2200 ± 1.9722 .This study involved partial purification of the MAO enzyme from patients' serum using Sephadex G 100. Which showed the degree of purification was (5.79, 16.37) weakness, the enzyme sum was (20.8, 50.37%) while the qualitative activity (2.45, 6.37 IU/mg). Studies were conducted to determine the values of Km and Vmax for MAO (1Χ10-5 M mm and 0.000285 IU/L) respectively and the optimal temperature (36 ° C) and pH (7.4). The molecular weight of the purified MAO enzyme from the serum of atherosclerosis patients (75,000 kD). The sedimentary method produced Fe3O4 nanoparticles identified by XRD, TEM and SEM. The study showed the effect of nanoscale Fe3O4 on the enzyme MAO NPs as a non-competitive inhibitor.