Invitro Activity of Novel Beta Lactam Antibiotic Ceftolazane/Tazobactam for Treatment of Patients Suffering from Bacteraemia Caused by Simple and MDR Strains of Pseudomonas Aeruginosa

Abstract

Background: Various bacteria are responsible for bacteraemia, including staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, salmonella species, group A streptococcus, and pseudomonas aeruginosa and it is evident by pathophysiology that bacteraemia may lead to sepsis, a life-threatening condition with organ dysfunction. Irrational use of antibiotics, over-the-counter use of antibiotics and misuse of antibiotics leads to production of emergence strains of bacteria that causes resistance to multiple antibiotics. Hence it is pertinent to study the activity of novel antibiotics against various organisms including pseudomonas aeruginosa.

Methodology: It was a quasi-experimental study, the calculated sample size was 385 which was extended to 500. The sample was recruited by consecutive sampling technique. Samples of blood received for culture and sensitivity at the microbiology laboratory were included in the study. The samples were inoculated for 5 days in BACTEC blood culture bottle having rich growth media followed by culture on blood and MacConkey Agar. After the growth of organism further confirmation was performed by gram staining, lactose fermentation analyses finally oxidase test was performed differentiate the Pseudomonas aeruginosa.

Results: Out of N=2398 samples submitted in the microbiology lab for blood culture during n=439 samples showed the growth of P. aeruginosa which is 18.3%. Out of these 439 samples 198 (45.2%) were simple strains and 241 (54.8%) were MDR strains of P. aeruginosa. Resistance was shown to be greater in MDR strains which included ceftolazane/tazobactam as well however, simple strains were sensitive to this drug.

Conclusion: MDR strains were seemed to be resistant to all available antibiotics including ceftolazane/tazobactam which is alarming and indicating that soon the MDR strains will develop as super bugs.