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Zucchini yellow mosaic virus (ZYMV) is one of the most destructive viruses which cause significant economic losses in many cucurbits crops in Egypt. The purpose of this study was to use biological and molecular techniques to characterize zucchini yellow mosaic virus (ZYMV) strain isolated from open fields of Sharqia Governorate and evalute some plant lectin extracts as antiviral agents against ZYMV. ZYMV was isolated from infected zucchini plants showing virus like symptoms, samples were collected from open fields of Sharqia Governorate In different seasons (2019-2021). The fruit samples of zucchini exhibited severe mosaic, blisters, yellowing and malformation. The isolated virus was detected biologically to different species belonging 3 families as (Cucurbitaceae, Chenopodiaceae, and Solanaceae)by mechanical inoculation, serologically by DAS-ELISA (Double antibody sandwich enzyme linked immunosorbent assay) and also Purified virus sample was negatively stained and examined under electron microscopy (EM) revealed flexuous filamentous particles approximately (680-840) nm long and (12-14) nm in diameter. Additionally, ultrathin sections of infected zucchini leaves showedamorphous and pinweels bodies in the cytoplasm. Histological changes showed that the number of chloroplasts decreased, deformation in the shape of cell wall. Systemic Acquired Resistance (SAR) by plant extracts against ZYMV infection results in reducing the symptoms by a percentage reached 90%.