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An important area of nanotechnology is the synthesis, characterization, and application of biologically generated nanomaterials. Due to the great chemical and thermal stability of gold nanoparticles and their potential applications in medicine due to their environmentally benign approach and cheap cost methodologies, biological production of gold nanoparticles has drawn a lot of attention and been the subject of research. In the current study, red marine algae Gracilaria corticata aqueous extract was used as a reducing agent to create gold nanoparticles. Scanning electron microscopy and UV-visible spectroscopy were used to demonstrate the production of gold nanoparticles (SEM). The synthesised gold nanoparticles were tested for their ability to scavenge free radicals by using the DPPH free radical scavenging assay and the ferric-ion reducing ability antioxidant power assay, as well as against the bacterial pathogens Staphylococcus aureus, Enterococcus faecalis and Enterobacter aerogenes, which are gramme positive and gramme negative, respectively. In comparison to the norms, the gold nanoparticles' antibacterial and antioxidant properties displayed a notable level of activity. These findings not only offer a sustainable method for creating nanoparticles, but they also pave the way for brand-new drug leads.