Development of Home-Made Mini-Fluorometer as a Detector for Semi Automated flow Injection System

Abstract

This study included the development of an inexpensive homemade flow injection system supplied with a miniFluorometer as a detector for determination of fluorescein dye.It was built and designed with the aid of a 3D printer. The unique features of fluorescein dye, which is excited and emitted in the visible spectrum, allow the optical arraignment of two LEDs, one as a source for excitation and a photo-resistant detector as a detector for collecting thefluorescent light. A single channel manifold was used to attain a simple and easy to use flow system in an educational laboratory. Two microcontrollers were used to operate this system; UNO and Mega types, to control the homemade mini-pump and as a data logger, respectively.Under the optimum experimental conditions, the standard calibration curve was found and the linearity was within the range of (0.2x10-4–1.6x10-4 mol/L with a correlation coefficient of 0.9990. The R.S.D was (0.81%) for ten replicates and the sample throughput was (200 samples/h).