Main Article Content
This study aimed to use pyocyanin pigment produced by the clinical isolate Pseudomonas aeruginosa as a reducing and stabilizing agent for core-shell nanoparticles of cobalt and titanium (Co-TiO2) and their antibacterial effectiveness against biofilm-forming, multidrug-resistant Escherichia coli obtained from diverse clinical sources. (Co-TiO2) NPs The preparation was in two phases, The first stage is the preparation of Cobalt nanoparticles by using cobaltous chloride hexahydrate (5gm) dispersing in (50ml) of pyocyanin, The second phase adds titanium tetrachloride (1ml) on pyocyanin (150 ml) that contains Cobalt nanoparticles Which were previously prepared. The (Co-TiO2) NPs synthesized were characterized by various techniques such as UV-VIS, AFM, XRD, FTIR, FE-SEM, and TEM. based on the wavelength of the Cobalt and titanium (Co-TiO2) core-shell nanoparticles at (284 nm) by spectrophotometer, The diameter was identified by Atomic force microscopy (AFM) the average diameter at 75.70 nm, and the higher concentration of (Co-TiO2) NPS in the solution at 0.1 mg/ml It was shown that the maximum inhibitory zones for E. Coli were 20 millimeters in diameter. (Co-TiO2) core-shell nanoparticles have been demonstrated to be effective against E. Coli that produce biofilms.