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Quinolone Susceptibility and Detection of qnr and aac(6’)-Ib-cr Genes in Community Isolates of Klebsiella pneumoniae

AUTHORS

Seyed Mohsen Seyedpour 1 , fereshteh eftekhar 1 , *

AUTHORS INFORMATION

1 Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Tehran, IR Iran

How to Cite: Seyedpour S M, eftekhar F. Quinolone Susceptibility and Detection of qnr and aac(6’)-Ib-cr Genes in Community Isolates of Klebsiella pneumoniae, Jundishapur J Microbiol. 2014 ; 7(7):e94146. doi: 10.5812/jjm.11136.

ARTICLE INFORMATION

Jundishapur Journal of Microbiology: 7 (7); e94146
Published Online: July 01, 2014
Article Type: Research Article
Received: May 20, 2019
Accepted: August 01, 2013
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Abstract

Background: Plasmid-mediated quinolone resistance genes (PMQR) have been shown to play not only an important role in quinolone resistance, but also resistance to other antibiotics, particularly β-lactams and aminoglycosides. These genes are mainly associated with clinical isolates of Enterobacteriaceae. However, detection of PMQR genes in the community isolates can increase the dissemination rate of resistance determinants among bacteria.

Objectives: This study aimed to investigate quinolone resistance and distribution of qnr and aac (6’)-Ib-cr genes among the community isolates of Klebsiella pneumoniae.

Materials and Methods: Fifty-two K. pneumoniae isolates were collected from the Central Laboratory in Karaj between July 2010 and January 2011. Antibacterial susceptibility was determined by the disc diffusion method. Quinolone and/or cephalosporin-resistant isolates were screened for the presence of qnrA, qnrB, qnrS and aac (6’)-Ib-cr genes by polymerase chain reaction (PCR).

Results: Of the 52 K. pneumoniae isolates, 23 were resistant to cephalosporins and/or quinolones. Overall, 7 out of the 23 resistant isolates harbored qnr and/or aac (6’)-Ib-cr genes (30.4%). Among these, 5 isolates were resistant to both classes of antibiotics of which; 3 carried the aac (6’)-Ib-cr gene, one had the qnrS, and one harbored both aac (6’)-Ib-cr and qnrB genes. None of the isolates contained qnrA. Two isolates were sensitive to quinolones and resistant to cephalosporins of which; one had qnrS and the other carried the aac (6’)-Ib-cr gene.

Conclusions: Our study showed that 30.4% of the quinolone and/or cephalosporin resistant community isolates of K. pneumoniaecarried PMQR genes. These results confirm that community isolates can be an important source for spreading antibiotic resistance determinants among Gram negative pathogens. This is the first report from Iran on detection of PMQR in the community isolates of K. pneumoniae.

Keywords

Klebsiella Pneumonia Quinolone Resistance PMQR qnr aac (6’)-Ib-cr qnr Genes

© 2014, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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