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Virulence Gene’s Relationship With Biofilm Formation and Detection of aac (6’)/aph (2”) in Enterococcus faecalis Isolated From Patients With Urinary Tract Infection

AUTHORS

Rezvan Moniri 1 , 2 , * , Ahmad Ghasemi 1 , Seyed Gholam Abbas Moosavi 3 , Kamran Dastehgoli 2 , Maryam Rezaei 1

AUTHORS INFORMATION

1 Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran

2 Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, IR Iran

3 Department of Statistic , Health Faculty, Kashan University of Medical Sciences, Kashan, IR Iran

How to Cite: Moniri R, Ghasemi A, Moosavi S G A, Dastehgoli K, Rezaei M. Virulence Gene’s Relationship With Biofilm Formation and Detection of aac (6’)/aph (2”) in Enterococcus faecalis Isolated From Patients With Urinary Tract Infection, Jundishapur J Microbiol. 2013 ; 6(5):e94137. doi: 10.5812/jjm.6244.

ARTICLE INFORMATION

Jundishapur Journal of Microbiology: 6 (5); e94137
Published Online: July 01, 2013
Article Type: Research Article
Received: May 20, 2019
Accepted: November 07, 2012
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Abstract

Objectives: We investigated the potential relationships between biofilm formation and prevalence of virulence genes (asa1, esp, cylA, and gelE/sprE), and antimicrobial resistance genes (aac (6’)/aph (2”) in Enterococcus faecalis isolated from patients with urinary tract infection.

Patients and Methods: In this survey 95 E. faecalis isolates from patients with urinary tract infections staying at Shahid Beheshti hospital in Kashan, Iran, between 2007 and 2008 were studied. We analyzed the prevalence of genes encoding virulence factors (asa1, esp, cylA and gelE/ sprE), and antimicrobial resistance genes [(aac (6’)/aph (2”)] by PCR. In addition, the production of biofilm and extracellular enzymes, hemolysin (Hln) and Gelatinase were examined.

Results: The asa1,(aac (6’)/aph (2”)esp, cylA, and gelE/sprE were detected in 94.7%, 68.4%, 61.1%, 50.5% and 21.1% of E.faecalis isolates, respectively. The hemolysin production and gelatinase activity were seen in 44.2% and 20% of isolates, respectively. 16.8% of E. faecalis isolates showed strong and 83.2% exhibited weak biofilm formation. The percentages of genes encoding virulence factors in E. faecalis which had the ability of strong biofilm formation were as follows: gelE/sprE 25%, esp22.4%, (aac (6’)/aph (2”)18.5%, asa1 16.7% and cylA 14.6%. The presence of both aac (6’)/aph (2”) and esp positive act as a risk factor for biofilm formation (P value < 0.001).

Conclusions: There was a significant relationship between biofilm formation and possession of esp and aac (6’)/aph (2”) genes. There was no evidence between biofilm formation and presence of any other gene. Enterococcal infections associated with biofilm formation have been a serious problem in recent years.

Keywords

Enterococcus faecalis Biofilms Urinary Tract Infection

© 2013, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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