Jundishapur Journal of Microbiology

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Visual and Rapid Detection of Klebsiella pneumoniae by Magnetic Immunocapture-Loop-Mediated Isothermal Amplification Assay

Liding Zhang 1 , Yaoqiang Shi 1 , Congjie Chen 1 , Qinqin Han 1 , Mi Zhang 1 , Huashan Yi 2 , Yuzhu Song 1 , Xueshan Xia 1 and Jinyang Zhang ORCID 1 , *
Authors Information
1 Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China
2 College of Animal Science, Southwest University, Rongchang, Chongqing, China
Article information
  • Jundishapur Journal of Microbiology: April 30, 2019, 12 (4); e90016
  • Published Online: April 29, 2019
  • Article Type: Research Article
  • Received: January 29, 2019
  • Revised: March 25, 2019
  • Accepted: March 31, 2019
  • DOI: 10.5812/jjm.90016

To Cite: Zhang L, Shi Y , Chen C, Han Q , Zhang M, et al. Visual and Rapid Detection of Klebsiella pneumoniae by Magnetic Immunocapture-Loop-Mediated Isothermal Amplification Assay, Jundishapur J Microbiol. 2019 ; 12(4):e90016. doi: 10.5812/jjm.90016.

Abstract
Copyright © 2019, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Xie Y, Tian L, Li G, Qu H, Sun J, Liang W, et al. Emergence of the third-generation cephalosporin-resistant hypervirulent Klebsiella pneumoniae due to the acquisition of a self-transferable blaDHA-1-carrying plasmid by an ST23 strain. Virulence. 2018;9(1):838-44. doi: 10.1080/21505594.2018.1456229. [PubMed: 29683780]. [PubMed Central: PMC5955457].
  • 2. Harada S, Ishii Y, Saga T, Aoki K, Tateda K. Molecular epidemiology of Klebsiella pneumoniae K1 and K2 isolates in Japan. Diagn Microbiol Infect Dis. 2018;91(4):354-9. doi: 10.1016/j.diagmicrobio.2018.03.010. [PubMed: 29678299].
  • 3. Lee CR, Lee JH, Park KS, Jeon JH, Kim YB, Cha CJ, et al. Antimicrobial resistance of hypervirulent Klebsiella pneumoniae: Epidemiology, hypervirulence-associated determinants, and resistance mechanisms. Front Cell Infect Microbiol. 2017;7:483. doi: 10.3389/fcimb.2017.00483. [PubMed: 29209595]. [PubMed Central: PMC5702448].
  • 4. Zhang H, Yang Q, Liao K, Ni Y, Yu Y, Hu B, et al. Update of incidence and antimicrobial susceptibility trends of Escherichia coli and Klebsiella pneumoniae isolates from Chinese intra-abdominal infection patients. BMC Infect Dis. 2017;17(1):776. doi: 10.1186/s12879-017-2873-z. [PubMed: 29254478]. [PubMed Central: PMC5735800].
  • 5. Wu H, Li D, Zhou H, Sun Y, Guo L, Shen D. Bacteremia and other body site infection caused by hypervirulent and classic Klebsiella pneumoniae. Microb Pathog. 2017;104:254-62. doi: 10.1016/j.micpath.2017.01.049. [PubMed: 28132768].
  • 6. Prokesch BC, TeKippe M, Kim J, Raj P, TeKippe EM, Greenberg DE. Primary osteomyelitis caused by hypervirulent Klebsiella pneumoniae. Lancet Infect Dis. 2016;16(9):e190-5. doi: 10.1016/S1473-3099(16)30021-4. [PubMed: 27402393].
  • 7. Martin RM, Bachman MA. Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front Cell Infect Microbiol. 2018;8:4. doi: 10.3389/fcimb.2018.00004. [PubMed: 29404282]. [PubMed Central: PMC5786545].
  • 8. Vuotto C, Longo F, Pascolini C, Donelli G, Balice MP, Libori MF, et al. Biofilm formation and antibiotic resistance in Klebsiella pneumoniae urinary strains. J Appl Microbiol. 2017;123(4):1003-18. doi: 10.1111/jam.13533. [PubMed: 28731269].
  • 9. Navon-Venezia S, Kondratyeva K, Carattoli A. Klebsiella pneumoniae: A major worldwide source and shuttle for antibiotic resistance. FEMS Microbiol Rev. 2017;41(3):252-75. doi: 10.1093/femsre/fux013. [PubMed: 28521338].
  • 10. Tangden T, Giske CG. Global dissemination of extensively drug-resistant carbapenemase-producing Enterobacteriaceae: Clinical perspectives on detection, treatment and infection control. J Intern Med. 2015;277(5):501-12. doi: 10.1111/joim.12342. [PubMed: 25556628].
  • 11. Yan J, Pu S, Jia X, Xu X, Yang S, Shi J, et al. Multidrug resistance mechanisms of carbapenem resistant Klebsiella pneumoniae strains isolated in Chongqing, China. Ann Lab Med. 2017;37(5):398-407. doi: 10.3343/alm.2017.37.5.398. [PubMed: 28643488]. [PubMed Central: PMC5500738].
  • 12. Zhang Y, Sun J, Mi C, Li W, Zhao S, Wang Q, et al. First report of two rapid-onset fatal infections caused by a newly emerging hypervirulent K. Pneumonia ST86 strain of serotype K2 in China. Front Microbiol. 2015;6:721. doi: 10.3389/fmicb.2015.00721. [PubMed: 26257712]. [PubMed Central: PMC4508851].
  • 13. Dong D, Liu W, Li H, Wang Y, Li X, Zou D, et al. Survey and rapid detection of Klebsiella pneumoniae in clinical samples targeting the rcsA gene in Beijing, China. Front Microbiol. 2015;6:519. doi: 10.3389/fmicb.2015.00519. [PubMed: 26052327]. [PubMed Central: PMC4440914].
  • 14. Nakano R, Nakano A, Ishii Y, Ubagai T, Kikuchi-Ueda T, Kikuchi H, et al. Rapid detection of the Klebsiella pneumoniae carbapenemase (KPC) gene by loop-mediated isothermal amplification (LAMP). J Infect Chemother. 2015;21(3):202-6. doi: 10.1016/j.jiac.2014.11.010. [PubMed: 25529001].
  • 15. Legese MH, Weldearegay GM, Asrat D. Extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae among Ethiopian children. Infect Drug Resist. 2017;10:27-34. doi: 10.2147/IDR.S127177. [PubMed: 28182124]. [PubMed Central: PMC5279835].
  • 16. Wang CH, Lu PL, Liu EY, Chen YY, Lin FM, Lin YT, et al. Rapid identification of capsular serotype K1/K2 Klebsiella pneumoniae in pus samples from liver abscess patients and positive blood culture samples from bacteremia cases via an immunochromatographic strip assay. Gut Pathog. 2019;11:11. doi: 10.1186/s13099-019-0285-x. [PubMed: 30828389]. [PubMed Central: PMC6385414].
  • 17. Gupta V, Gulati P, Bhagat N, Dhar MS, Virdi JS. Detection of Yersinia enterocolitica in food: An overview. Eur J Clin Microbiol Infect Dis. 2015;34(4):641-50. doi: 10.1007/s10096-014-2276-7. [PubMed: 25410144].
  • 18. Paterson DL, Rice LB, Bonomo RA. Rapid method of extraction and analysis of extended-spectrum beta-lactamases from clinical strains of Klebsiella pneumoniae. Clin Microbiol Infect. 2001;7(12):709-11. doi: 10.1046/j.1469-0691.2001.00363.x. [PubMed: 11843918].
  • 19. Thirapanmethee K, Pothisamutyothin K, Nathisuwan S, Chomnawang MT, Wiwat C. Loop-mediated isothermal amplification assay targeting the blaCTX-M9 gene for detection of extended spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae. Microbiol Immunol. 2014;58(12):655-65. doi: 10.1111/1348-0421.12205. [PubMed: 25284314].
  • 20. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28(12). E63. doi: 10.1093/nar/28.12.e63. [PubMed: 10871386]. [PubMed Central: PMC102748].
  • 21. Li L, Yin D, Xu K, Liu Y, Song D, Wang J, et al. A sandwich immunoassay for brucellosis diagnosis based on immune magnetic beads and quantum dots. J Pharm Biomed Anal. 2017;141:79-86. doi: 10.1016/j.jpba.2017.03.002. [PubMed: 28432940].
  • 22. Ye Y, Li H, Wu Q, Na L, Han Y. Immunocaptured-loop mediated isothermal amplification assay for detection ofvibrio parahaemolyticusin seafood. J Food Saf. 2014;34(1):21-5. doi: 10.1111/jfs.12090.
  • 23. Zhang L, Wei Q, Han Q, Chen Q, Tai W, Zhang J, et al. Detection of shigella in milk and clinical samples by magnetic immunocaptured-loop-mediated isothermal amplification assay. Front Microbiol. 2018;9:94. doi: 10.3389/fmicb.2018.00094. [PubMed: 29467730]. [PubMed Central: PMC5807921].
  • 24. Zhang L, Du X, Chen C, Chen Z, Zhang L, Han Q, et al. Development and characterization of double-antibody sandwich ELISA for detection of zika virus infection. Viruses. 2018;10(11). doi: 10.3390/v10110634. [PubMed: 30445676]. [PubMed Central: PMC6266115].
  • 25. Zhang L, Chen , Dai L, Zhang L, Xu K, Song Y, et al. Efficient capture and detection of zika virion by polyclonal antibody against prokaryotic recombinant envelope protein. Jundishapur J Microbiol. 2018;11(10). e68858. doi: 10.5812/jjm.68858.
  • 26. Zhang L, Shi Y, Chen C, Han Q, Chen Q, Xia X, et al. Rapid, visual detection of Klebsiella pneumoniae using magnetic nanoparticles and an horseradish peroxidase-probe based immunosensor. J Biomed Nanotechnol. 2019;15(5):1061-71. doi: 10.1166/jbn.2019.2736. [PubMed: 30890236].
  • 27. Magill SS, Edwards JR, Bamberg W, Beldavs ZG, Dumyati G, Kainer MA, et al. Multistate point-prevalence survey of health care-associated infections. N Engl J Med. 2014;370(13):1198-208. doi: 10.1056/NEJMoa1306801. [PubMed: 24670166]. [PubMed Central: PMC4648343].
  • 28. Tzouvelekis LS, Markogiannakis A, Psichogiou M, Tassios PT, Daikos GL. Carbapenemases in Klebsiella pneumoniae and other Enterobacteriaceae: An evolving crisis of global dimensions. Clin Microbiol Rev. 2012;25(4):682-707. doi: 10.1128/CMR.05035-11. [PubMed: 23034326]. [PubMed Central: PMC3485753].
  • 29. European Centre for Disease Prevention and Control. Antibiotic resistance threats in the United States, 2013. 2013.
  • 30. Voulgari E, Poulou A, Koumaki V, Tsakris A. Carbapenemase-producing Enterobacteriaceae: Now that the storm is finally here, how will timely detection help us fight back? Future Microbiol. 2013;8(1):27-39. doi: 10.2217/fmb.12.130. [PubMed: 23252491].
  • 31. Zende R, Kshirsagar D, Vaidya V, Waghamare R, Todankar R, Shirke A. Loop-mediated isothermal amplification assay (LAMP): A rapid tool for diagnosis of foodborne and zoonotic pathogens: A review. Int J Livest Res. 2017;7(5):23-35. doi: 10.5455/ijlr.20170415114515.
  • 32. Song J, Mauk MG, Hackett BA, Cherry S, Bau HH, Liu C. Instrument-free point-of-care molecular detection of zika virus. Anal Chem. 2016;88(14):7289-94. doi: 10.1021/acs.analchem.6b01632. [PubMed: 27306491]. [PubMed Central: PMC4955015].
  • 33. Chen Q, Li Y, Tao T, Bie X, Lu F, Lu Z. Development and application of a sensitive, rapid, and reliable immunomagnetic separation-PCR detection method for Cronobacter spp. J Dairy Sci. 2017;100(2):961-9. doi: 10.3168/jds.2016-11087. [PubMed: 28012621].
  • 34. Gilardoni LR, Fernandez B, Morsella C, Mendez L, Jar AM, Paolicchi FA, et al. Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR. Braz J Microbiol. 2016;47(2):506-12. doi: 10.1016/j.bjm.2016.01.013. [PubMed: 26991290]. [PubMed Central: PMC4874612].
  • 35. Khazani NA, Noor NZ, Yean Yean C, Hasan H, Suraiya S, Mohamad S. A thermostabilized, one-step PCR assay for simultaneous detection of Klebsiella pneumoniae and haemophilus influenzae. J Trop Med. 2017;2017:7210849. doi: 10.1155/2017/7210849. [PubMed: 28386286]. [PubMed Central: PMC5366220].
  • 36. Tekintas Y, Cilli F, Erac B, Yasar M, Aydemir SS, Hosgor Limoncu M. [Comparison of phenotypic methods and polymerase chain reaction for the detection of carbapenemase production in clinical Klebsiella pneumoniae isolates]. Mikrobiyol Bul. 2017;51(3):269-76. Turkish. doi: 10.5578/mb.57333. [PubMed: 28929963].

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