Jundishapur Journal of Microbiology

Published by: Kowsar

The Complexity of the Identification of Burkholderia cepacia Strain Which Caused Septicemia

Lan Anh Thi Bui 1 , Irina Zakharova 2 , * , Ivan Shpak 2 , Natalya Teteryatnikova 2 , Dimitriy Ustinov 2 , Yulia Kuzyutina 2 , Ha Ngoc Nguyen 3 and Dmitry Viktorov 2
Authors Information
1 Department of Tropical Medicine, Russian‐Vietnamese Tropical Research Center, Hanoi, Vietnam
2 Department of Microbiology, Volgograd Plague Control Research Institute, Volgograd, Russia
3 Phu Tho Provincial General Hospital, Viet Tri, Vietnam
Article information
  • Jundishapur Journal of Microbiology: November 2018, 11 (11); e82834
  • Published Online: November 3, 2018
  • Article Type: Brief Report
  • Received: August 1, 2018
  • Revised: October 18, 2018
  • Accepted: October 20, 2018
  • DOI: 10.5812/jjm.82834

To Cite: Bui L A T, Zakharova I, Shpak I, Teteryatnikova N, Ustinov D, et al. The Complexity of the Identification of Burkholderia cepacia Strain Which Caused Septicemia, Jundishapur J Microbiol. 2018 ; 11(11):e82834. doi: 10.5812/jjm.82834.

Copyright © 2018, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Lipsitz R, Garges S, Aurigemma R, Baccam P, Blaney DD, Cheng AC, et al. Workshop on treatment of and postexposure prophylaxis for Burkholderia pseudomallei and B. mallei Infection, 2010. Emerg Infect Dis. 2012;18(12). e2. doi: 10.3201/eid1812.120638. [PubMed: 23171644]. [PubMed Central: PMC3557896].
  • 2. Brent AJ, Matthews PC, Dance DA, Pitt TL, Handy R. Misdiagnosing melioidosis. Emerg Infect Dis. 2007;13(2):349-51. doi: 10.3201/eid1302.061290. [PubMed: 17479916]. [PubMed Central: PMC2725863].
  • 3. Kiratisin P, Santanirand P, Chantratita N, Kaewdaeng S. Accuracy of commercial systems for identification of Burkholderia pseudomallei versus Burkholderia cepacia. Diagn Microbiol Infect Dis. 2007;59(3):277-81. doi: 10.1016/j.diagmicrobio.2007.06.013. [PubMed: 17916419].
  • 4. CLSI. Methods for antimicrobial dilution and disk susceptibility isolated or fastidious bacteria. CLSI guideline M45. 3rd ed. Wayne, PA, USA: Clinical and Laboratory Standards Institute; 2016.
  • 5. Zakharova I, Teteryatnikova N, Toporkov A, Viktorov D. Development of a multiplex PCR assay for the detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei, Burkholderia thailandensis, and Burkholderia cepacia complex. Acta Trop. 2017;174:1-8. doi: 10.1016/j.actatropica.2017.06.016. [PubMed: 28634144].
  • 6. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, et al. SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012;19(5):455-77. doi: 10.1089/cmb.2012.0021. [PubMed: 22506599]. [PubMed Central: PMC3342519].
  • 7. Spilker T, Baldwin A, Bumford A, Dowson CG, Mahenthiralingam E, LiPuma JJ. Expanded multilocus sequence typing for Burkholderia species. J Clin Microbiol. 2009;47(8):2607-10. doi: 10.1128/JCM.00770-09. [PubMed: 19494070]. [PubMed Central: PMC2725695].
  • 8. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016;33(7):1870-4. doi: 10.1093/molbev/msw054. [PubMed: 27004904].
  • 9. Deepak RN, Crawley B, Phang E. Burkholderia pseudomallei identification: A comparison between the API 20NE and VITEK2GN systems. Trans R Soc Trop Med Hyg. 2008;102 Suppl 1:S42-4. doi: 10.1016/S0035-9203(08)70012-9. [PubMed: 19121685].
  • 10. Weissert C, Dollenmaier G, Rafeiner P, Riehm J, Schultze D. Burkholderia pseudomallei misidentified by automated system. Emerg Infect Dis. 2009;15(11):1799-801. doi: 10.3201/eid1511.081719. [PubMed: 19891868]. [PubMed Central: PMC2857215].
  • 11. Podin Y, Kaestli M, McMahon N, Hennessy J, Ngian HU, Wong JS, et al. Reliability of automated biochemical identification of Burkholderia pseudomallei is regionally dependent. J Clin Microbiol. 2013;51(9):3076-8. doi: 10.1128/JCM.01290-13. [PubMed: 23784129]. [PubMed Central: PMC3754659].
  • 12. Zakharova IB, Lopasteyskaya YA, Toporkov AV, Viktorov DV. Influence of biochemical features of Burkholderia pseudomallei strains on identification reliability by vitek 2 system. J Glob Infect Dis. 2018;10(1):7-10. doi: 10.4103/jgid.jgid_39_17. [PubMed: 29563716]. [PubMed Central: PMC5850765].
  • 13. Brisse S, Stefani S, Verhoef J, Van Belkum A, Vandamme P, Goessens W. Comparative evaluation of the BD Phoenix and VITEK 2 automated instruments for identification of isolates of the Burkholderia cepacia complex. J Clin Microbiol. 2002;40(5):1743-8. [PubMed: 11980954]. [PubMed Central: PMC130956].
  • 14. Mahenthiralingam E, Bischof J, Byrne SK, Radomski C, Davies JE, Av-Gay Y, et al. DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J Clin Microbiol. 2000;38(9):3165-73. [PubMed: 10970351]. [PubMed Central: PMC87345].
  • 15. Jimenez L, Jashari T, Vasquez J, Zapata S, Bochis J, Kulko M, et al. Real-time PCR detection of Burkholderia cepacia in pharmaceutical products contaminated with low levels of bacterial contamination. PDA J Pharm Sci Technol. 2018;72(1):73-80. doi: 10.5731/pdajpst.2017.007971. [PubMed: 29030528].
  • 16. Suppiah J, Thimma JS, Cheah SH, Vadivelu J. Development and evaluation of polymerase chain reaction assay to detect Burkholderia genus and to differentiate the species in clinical specimens. FEMS Microbiol Lett. 2010;306(1):9-14. doi: 10.1111/j.1574-6968.2010.01923.x. [PubMed: 20345378].
  • 17. Koh SF, Tay ST, Sermswan R, Wongratanacheewin S, Chua KH, Puthucheary SD. Development of a multiplex PCR assay for rapid identification of Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex. J Microbiol Methods. 2012;90(3):305-8. doi: 10.1016/j.mimet.2012.06.002. [PubMed: 22705921].

Featured Image:

Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments