Jundishapur Journal of Microbiology

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Assessment of Metronidazole and Clarithromycin Resistance Among Helicobacter pylori Isolates of Ahvaz (Southwest of Iran) During 2015 - 2016 by Phenotypic and Molecular Methods

Mansour Amin 1 , Ali Akbar Shayesteh 2 , Amirarsalan Serajian 1 , * and Hamed Goodarzi 1
Authors Information
1 Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Research Center for Infectious Diseases of Digestive System (Alimentary Tract Research Center), Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Article information
  • Jundishapur Journal of Microbiology: 12 (4); e80156
  • Published Online: April 10, 2019
  • Article Type: Research Article
  • Received: June 3, 2018
  • Revised: March 7, 2019
  • Accepted: March 14, 2019
  • DOI: 10.5812/jjm.80156

To Cite: Amin M, Shayesteh A A, Serajian A, Goodarzi H. Assessment of Metronidazole and Clarithromycin Resistance Among Helicobacter pylori Isolates of Ahvaz (Southwest of Iran) During 2015 - 2016 by Phenotypic and Molecular Methods, Jundishapur J Microbiol. Online ahead of Print ; 12(4):e80156. doi: 10.5812/jjm.80156.

Copyright © 2019, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Guo C, Liao Y, Li Y, Duan J, Guo Y, Wu Y, et al. Genotyping analysis of Helicobacter pylori using multiple-locus variable-number tandem-repeats analysis in five regions of China and Japan. BMC Microbiol. 2011;11:197. doi: 10.1186/1471-2180-11-197. [PubMed: 21888662]. [PubMed Central: PMC3178492].
  • 2. Vega AE, Cortinas TI, Puig ON, Silva HJ. Molecular characterization and susceptibility testing of Helicobacter pylori strains isolated in western Argentina. Int J Infect Dis. 2010;14 Suppl 3:e85-92. doi: 10.1016/j.ijid.2009.11.022. [PubMed: 20304694].
  • 3. Ghorbani F, Gheisari E, Safarpoor Dehkordi F. Genotyping of vacA alleles of Helicobacter pylori strains recovered from some Iranian food items. Trop J Pharm Res. 2016;15(8):1631-6. doi: 10.4314/tjpr.v15i8.5.
  • 4. Alhussaini MS. among patients with different gastrointestinal disorders in Saudi Arabia. Med J Indones. 2017;25(4):214. doi: 10.13181/mji.v25i4.1442.
  • 5. Bridge DR, Merrell DS. Polymorphism in the Helicobacter pylori CagA and VacA toxins and disease. Gut Microbes. 2013;4(2):101-17. doi: 10.4161/gmic.23797. [PubMed: 23380646]. [PubMed Central: PMC3595067].
  • 6. Santos JC, Ladeira MS, Pedrazzoli JJ, Ribeiro ML. Relationship of IL-1 and TNF-alpha polymorphisms with Helicobacter pylori in gastric diseases in a Brazilian population. Braz J Med Biol Res. 2012;45(9):811-7. doi: 10.1590/S0100-879X2012007500099. [PubMed: 22714811]. [PubMed Central: PMC3854325].
  • 7. Taghvaei T. [An investigation of the prevalence of iceA genotypes in Helicobacter pylori strains isolated from peptic ulcer patients in Sari (2008)]. Arak Med Univ J. 2010;13(3):84-90. Persian.
  • 8. Vaziri F, Najar Peerayeh S, Alebouyeh M, Mirzaei T, Yamaoka Y, Molaei M, et al. Diversity of Helicobacter pylori genotypes in Iranian patients with different gastroduodenal disorders. World J Gastroenterol. 2013;19(34):5685-92. doi: 10.3748/wjg.v19.i34.5685. [PubMed: 24039362]. [PubMed Central: PMC3769906].
  • 9. Shiota S, Cruz M, Abreu JA, Mitsui T, Terao H, Disla M, et al. Virulence genes of Helicobacter pylori in the Dominican Republic. J Med Microbiol. 2014;63(Pt 9):1189-96. doi: 10.1099/jmm.0.075275-0. [PubMed: 24965801]. [PubMed Central: PMC4140083].
  • 10. Tanih NF, Ndip RN. Molecular Detection of Antibiotic Resistance in South African Isolates of Helicobacter pylori. Gastroenterol Res Pract. 2013;2013:1-6. doi: 10.1155/2013/259457.
  • 11. Wolle K, Leodolter A, Malfertheiner P, Konig W. Antibiotic susceptibility of Helicobacter pylori in Germany: Stable primary resistance from 1995 to 2000. J Med Microbiol. 2002;51(8):705-9. doi: 10.1099/0022-1317-51-8-705. [PubMed: 12171304].
  • 12. Wu H, Shi XD, Wang HT, Liu JX. Resistance of helicobacter pylori to metronidazole, tetracycline and amoxycillin. J Antimicrob Chemother. 2000;46(1):121-3. [PubMed: 10882700].
  • 13. De Francesco V, Giorgio F, Hassan C, Manes G, Vannella L, Panella C, et al. Worldwide H. pylori antibiotic resistance: A systematic review. J Gastrointestin Liver Dis. 2010;19(4):409-14. [PubMed: 21188333].
  • 14. Wu W, Yang Y, Sun G. Recent insights into antibiotic resistance in Helicobacter pylori eradication. Gastroenterol Res Pract. 2012;2012:723183. doi: 10.1155/2012/723183. [PubMed: 22829809]. [PubMed Central: PMC3398622].
  • 15. Kim SY, Choi DJ, Chung JW. Antibiotic treatment for Helicobacter pylori: Is the end coming? World J Gastrointest Pharmacol Ther. 2015;6(4):183-98. doi: 10.4292/wjgpt.v6.i4.183. [PubMed: 26558152]. [PubMed Central: PMC4635158].
  • 16. Agudo S, Perez-Perez G, Alarcon T, Lopez-Brea M. Rapid detection of clarithromycin resistant Helicobacter pylori strains in Spanish patients by polymerase chain reaction-restriction fragment length polymorphism. Rev Esp Quimioter. 2011;24(1):32-6. [PubMed: 21412667]. [PubMed Central: PMC4060515].
  • 17. Klesiewicz K, Nowak P, Karczewska E, Skiba I, Wojtas-Bonior I, Sito E, et al. PCR-RFLP detection of point mutations A2143G and A2142G in 23S rRNA gene conferring resistance to clarithromycin in Helicobacter pylori strains. Acta Biochim Pol. 2014;61(2):311-5. doi: 10.18388/abp.2014_1901. [PubMed: 24927236].
  • 18. Cerqueira L, Fernandes RM, Ferreira RM, Oleastro M, Carneiro F, Brandao C, et al. Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of Helicobacter pylori clarithromycin resistance in gastric biopsy specimens. J Clin Microbiol. 2013;51(6):1887-93. doi: 10.1128/JCM.00302-13. [PubMed: 23596234]. [PubMed Central: PMC3716114].
  • 19. Owen RJ. Molecular testing for antibiotic resistance in Helicobacter pylori. Gut. 2002;50(3):285-9. doi: 10.1136/gut.50.3.285. [PubMed: 11839700]. [PubMed Central: PMC1773144].
  • 20. Papastergiou V, Georgopoulos SD, Karatapanis S. Treatment of Helicobacter pylori infection: Meeting the challenge of antimicrobial resistance. World J Gastroenterol. 2014;20(29):9898-911. doi: 10.3748/wjg.v20.i29.9898. [PubMed: 25110420]. [PubMed Central: PMC4123371].
  • 21. Testerman TL, Morris J. Beyond the stomach: An updated view of Helicobacter pylori pathogenesis, diagnosis, and treatment. World J Gastroenterol. 2014;20(36):12781-808. doi: 10.3748/wjg.v20.i36.12781. [PubMed: 25278678]. [PubMed Central: PMC4177463].
  • 22. Butlop TR, Mungkote NT, Chaichanawongsaroj NT. Analysis of allelic variants of rdxA associated with metronidazole resistance in Helicobacter pylori: Detection of common genotypes in rdxA by multiplex allele-specific polymerase chain reaction. Genet Mol Res. 2016;15(3). doi: 10.4238/gmr.15038674. [PubMed: 27706703].
  • 23. Doroud D, Mohajerani N, Massarrat S, Mohammadi M. Are molecular methods appropriate substitutions for traditional antimicrobial assays in detecting Helicobacter pylori antibiotic resistance in Iranian clinical trials? The 4th National Biotechnology Congress Islamic Republic of Iran. Kerman. 2005.
  • 24. Lui SY, Ling KL, Ho B. rdxA. Gene is an unlikely marker for metronidazole resistance in the Asian Helicobacter pylori isolates. J Microbiol Biotechnol. 2003;13(5):751-8.
  • 25. Jeong JY, Mukhopadhyay AK, Akada JK, Dailidiene D, Hoffman PS, Berg DE. Roles of FrxA and RdxA nitroreductases of Helicobacter pylori in susceptibility and resistance to metronidazole. J Bacteriol. 2001;183(17):5155-62. doi: 10.1128/JB.183.17.5155-5162.2001. [PubMed: 11489869]. [PubMed Central: PMC95392].
  • 26. Siavoshi F, Saniee P, Latifi-Navid S, Massarrat S, Sheykholeslami A. Increase in resistance rates of H. pylori isolates to metronidazole and tetracycline--comparison of three 3-year studies. Arch Iran Med. 2010;13(3):177-87. [PubMed: 20433221].
  • 27. Forbes B, Sahm D, Weissfeld A. Bailey' Scottʼs Diagnostic microbiology. 12th ed. USA: MOSBEY-ELSEVIER; 2007.
  • 28. Menoni SM, Bonon SH, Zeitune JM, Costa SC. PCR-based detection and genotyping of Helicobacter pylori in endoscopic biopsy samples from Brazilian patients. Gastroenterol Res Pract. 2013;2013:951034. doi: 10.1155/2013/951034. [PubMed: 23401678]. [PubMed Central: PMC3562691].
  • 29. Atapoor S, Safarpoor Dehkordi F, Rahimi E. Detection of Helicobacter pylori in various types of vegetables and salads. Jundishapur J Microbiol. 2014;7(5). e10013. doi: 10.5812/jjm.10013. [PubMed: 25147709]. [PubMed Central: PMC4138632].
  • 30. Zhao LJ, Huang YQ, Chen BP, Mo XQ, Huang ZS, Huang XF, et al. Helicobacter pylori isolates from ethnic minority patients in Guangxi: Resistance rates, mechanisms, and genotype. World J Gastroenterol. 2014;20(16):4761-70. doi: 10.3748/wjg.v20.i16.4761. [PubMed: 24782630]. [PubMed Central: PMC4000514].
  • 31. Abdollahi H, Savari M, Zahedi MJ, Moghadam SD, Hayatbakhsh Abasi M. Detection of A2142C, A2142G, and A2143G mutations in 23s rRNA gene conferring resistance to clarithromycin among Helicobacter pylori isolates in Kerman, Iran. Iran J Med Sci. 2011;36(2):104-10. [PubMed: 23359224]. [PubMed Central: PMC3556758].
  • 32. Chattopadhyay S, Patra R, Ramamurthy T, Chowdhury A, Santra A, Dhali GK, et al. Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens. J Clin Microbiol. 2004;42(6):2821-4. doi: 10.1128/JCM.42.6.2821-2824.2004. [PubMed: 15184482]. [PubMed Central: PMC427847].
  • 33. Miftahussurur M, Yamaoka Y. Diagnostic methods of Helicobacter pylori infection for epidemiological studies: Critical importance of indirect test validation. Biomed Res Int. 2016;2016:4819423. doi: 10.1155/2016/4819423. [PubMed: 26904678]. [PubMed Central: PMC4745376].
  • 34. Rasheed F, Campbell BJ, Alfizah H, Varro A, Zahra R, Yamaoka Y, et al. Analysis of clinical isolates of Helicobacter pylori in Pakistan reveals high degrees of pathogenicity and high frequencies of antibiotic resistance. Helicobacter. 2014;19(5):387-99. doi: 10.1111/hel.12142. [PubMed: 24827414]. [PubMed Central: PMC4162849].
  • 35. Secka O, Berg DE, Antonio M, Corrah T, Tapgun M, Walton R, et al. Antimicrobial susceptibility and resistance patterns among Helicobacter pylori strains from The Gambia, West Africa. Antimicrob Agents Chemother. 2013;57(3):1231-7. doi: 10.1128/AAC.00517-12. [PubMed: 23263004]. [PubMed Central: PMC3591906].
  • 36. Liou JM, Chang CY, Chen MJ, Chen CC, Fang YJ, Lee JY, et al. The primary resistance of Helicobacter pylori in Taiwan after the national policy to restrict antibiotic consumption and its relation to virulence factors-a nationwide study. PLoS One. 2015;10(5). e0124199. doi: 10.1371/journal.pone.0124199. [PubMed: 25942450]. [PubMed Central: PMC4420283].
  • 37. Shokrzadeh L, Jafari F, Dabiri H, Baghaei K, Zojaji H, Alizadeh AH, et al. Antibiotic susceptibility profile of Helicobacter pylori isolated from the dyspepsia patients in Tehran, Iran. Saudi J Gastroenterol. 2011;17(4):261-4. doi: 10.4103/1319-3767.82581. [PubMed: 21727733]. [PubMed Central: PMC3133984].
  • 38. Lottspeich C, Schwarzer A, Panthel K, Koletzko S, Russmann H. Evaluation of the novel Helicobacter pylori ClariRes real-time PCR assay for detection and clarithromycin susceptibility testing of H. pylori in stool specimens from symptomatic children. J Clin Microbiol. 2007;45(6):1718-22. doi: 10.1128/JCM.00103-07. [PubMed: 17392440]. [PubMed Central: PMC1933074].
  • 39. Caliskan R, Tokman HB, Erzin Y, Saribas S, Yuksel P, Bolek BK, et al. Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey. Rev Soc Bras Med Trop. 2015;48(3):278-84. doi: 10.1590/0037-8682-0027-2015. [PubMed: 26108005].
  • 40. Safavi M, Sabourian R, Foroumadi A. Treatment of Helicobacter pylori infection: Current and future insights. World J Clin Cases. 2016;4(1):5-19. doi: 10.12998/wjcc.v4.i1.5. [PubMed: 26798626]. [PubMed Central: PMC4714294].
  • 41. Nishizawa T, Suzuki H. Mechanisms of Helicobacter pylori antibiotic resistance and molecular testing. Front Mol Biosci. 2014;1:19. doi: 10.3389/fmolb.2014.00019. [PubMed: 25988160]. [PubMed Central: PMC4428472].
  • 42. Mirzaei N, Poursina F, Moghim S, Rahimi E, Safaei HG. The mutation of the rdxA gene in metronidazole-resistant Helicobacter pylori clinical isolates. Adv Biomed Res. 2014;3:90. doi: 10.4103/2277-9175.128469. [PubMed: 24761398]. [PubMed Central: PMC3988589].

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