Jundishapur Journal of Microbiology

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Antibiotic Characterization of Acinetobacter baumannii Isolated from Clinical Samples and Production of Recombinant OmpA from Resistant Strains

Maryam Tahmasebi Birgani 1,2 , Mahdi Bijanzadeh 1 and Hossein Ansari 3 , *
Authors Information
1 Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Iran
3 Department of Biotechnology, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
Article information
  • Jundishapur Journal of Microbiology: 11 (12); e78773
  • Published Online: November 20, 2018
  • Article Type: Research Article
  • Received: May 13, 2018
  • Revised: September 24, 2018
  • Accepted: October 18, 2018
  • DOI: 10.5812/jjm.78773

To Cite: Tahmasebi Birgani M, Bijanzadeh M, Ansari H. Antibiotic Characterization of Acinetobacter baumannii Isolated from Clinical Samples and Production of Recombinant OmpA from Resistant Strains, Jundishapur J Microbiol. Online ahead of Print ; 11(12):e78773. doi: 10.5812/jjm.78773.

Abstract
Copyright © 2018, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Gordon NC, Wareham DW. Multidrug-resistant Acinetobacter baumannii: Mechanisms of virulence and resistance. Int J Antimicrob Agents. 2010;35(3):219-26. doi: 10.1016/j.ijantimicag.2009.10.024. [PubMed: 20047818].
  • 2. Ansari H, Doosti A, Kargar M, Bijanzadeh M, Jaafarinia M. Cloning of ompA gene from Acinetobacter baumannii into the eukaryotic expression vector pBudCE4.1 as DNA vaccine. Indian J Microbiol. 2018;58(2):174-81. doi: 10.1007/s12088-017-0705-x. [PubMed: 29651176]. [PubMed Central: PMC5891471].
  • 3. Bergogne-Berezin E, Towner KJ. Acinetobacter spp. as nosocomial pathogens: Microbiological, clinical, and epidemiological features. Clin Microbiol Rev. 1996;9(2):148-65. [PubMed: 8964033]. [PubMed Central: PMC172888].
  • 4. Ansari H, Doosti A, Kargar M, Bizhanzadeh M, Jafarinya M. [Cloning and sequencing of the ompA and smpA virulence genes of Acentobacter baumannii Isolated in clinical samples]. Armaghane Danesh. 2017;21(12):1207-17. Persian.
  • 5. Snitkin ES, Zelazny AM, Montero CI, Stock F, Mijares L, Nisc Comparative Sequence Program , et al. Genome-wide recombination drives diversification of epidemic strains of Acinetobacter baumannii. Proc Natl Acad Sci U S A. 2011;108(33):13758-63. doi: 10.1073/pnas.1104404108. [PubMed: 21825119]. [PubMed Central: PMC3158218].
  • 6. Lin MF, Lan CY. Antimicrobial resistance in Acinetobacter baumannii: From bench to bedside. World J Clin Cases. 2014;2(12):787-814. doi: 10.12998/wjcc.v2.i12.787. [PubMed: 25516853]. [PubMed Central: PMC4266826].
  • 7. Lee HW, Koh YM, Kim J, Lee JC, Lee YC, Seol SY, et al. Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces. Clin Microbiol Infect. 2008;14(1):49-54. doi: 10.1111/j.1469-0691.2007.01842.x. [PubMed: 18005176].
  • 8. Huang W, Yao Y, Wang S, Xia Y, Yang X, Long Q, et al. Immunization with a 22-kDa outer membrane protein elicits protective immunity to multidrug-resistant Acinetobacter baumannii. Sci Rep. 2016;6:20724. doi: 10.1038/srep20724. [PubMed: 26853590]. [PubMed Central: PMC4745112].
  • 9. Garcia-Garmendia JL, Ortiz-Leyba C, Garnacho-Montero J, Jimenez-Jimenez FJ, Perez-Paredes C, Barrero-Almodovar AE, et al. Risk factors for Acinetobacter baumannii nosocomial bacteremia in critically ill patients: A cohort study. Clin Infect Dis. 2001;33(7):939-46. doi: 10.1086/322584. [PubMed: 11528563].
  • 10. Howard A, O'Donoghue M, Feeney A, Sleator RD. Acinetobacter baumannii: An emerging opportunistic pathogen. Virulence. 2012;3(3):243-50. doi: 10.4161/viru.19700. [PubMed: 22546906]. [PubMed Central: PMC3442836].
  • 11. Alzubaidi AN, Alkozai ZM. Immunogenic properties of outer membrane protein of Acinetobacter baumannii that loaded on chitosan nanoparticles. Am J Biomed. 2015;3(2):59-74.
  • 12. Ada G. Overview of vaccines and vaccination. Mol Biotechnol. 2005;29(3):255-72. doi: 10.1385/mb:29:3:255.
  • 13. Nascimento IP, Leite LC. Recombinant vaccines and the development of new vaccine strategies. Braz J Med Biol Res. 2012;45(12):1102-11. [PubMed: 22948379]. [PubMed Central: PMC3854212].
  • 14. Andersson C. Production and delivery of recombinant subunit vaccines [dissertation]. Stockholm: Royal Institute of Technology (KTH); 2000.
  • 15. Williams JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: Impact on efficacy, safety and upstream production. Biotechnol Adv. 2009;27(4):353-70. doi: 10.1016/j.biotechadv.2009.02.003. [PubMed: 19233255]. [PubMed Central: PMC2693335].
  • 16. Page MG. The role of the outer membrane of Gram-negative bacteria in antibiotic resistance: Ajax' shield or Achilles' heel? Antibiotic resistance. Springer; 2012. p. 67-86. doi: 10.1007/978-3-642-28951-4_5.
  • 17. Choi CH, Lee EY, Lee YC, Park TI, Kim HJ, Hyun SH, et al. Outer membrane protein 38 of Acinetobacter baumannii localizes to the mitochondria and induces apoptosis of epithelial cells. Cell Microbiol. 2005;7(8):1127-38. doi: 10.1111/j.1462-5822.2005.00538.x. [PubMed: 16008580].
  • 18. Kuo HY, Chao HH, Liao PC, Hsu L, Chang KC, Tung CH, et al. Functional characterization of Acinetobacter baumannii lacking the RNA chaperone Hfq. Front Microbiol. 2017;8:2068. doi: 10.3389/fmicb.2017.02068. [PubMed: 29163381]. [PubMed Central: PMC5663733].
  • 19. Moriel DG, Beatson SA, Wurpel DJ, Lipman J, Nimmo GR, Paterson DL, et al. Identification of novel vaccine candidates against multidrug-resistant Acinetobacter baumannii. PLoS One. 2013;8(10). e77631. doi: 10.1371/journal.pone.0077631. [PubMed: 24116234]. [PubMed Central: PMC3792912].
  • 20. McConnell MJ, Dominguez-Herrera J, Smani Y, Lopez-Rojas R, Docobo-Perez F, Pachon J. Vaccination with outer membrane complexes elicits rapid protective immunity to multidrug-resistant Acinetobacter baumannii. Infect Immun. 2011;79(1):518-26. doi: 10.1128/IAI.00741-10. [PubMed: 20974823]. [PubMed Central: PMC3019872].
  • 21. Jeannin P, Magistrelli G, Goetsch L, Haeuw JF, Thieblemont N, Bonnefoy JY, et al. Outer membrane protein A (OmpA): A new pathogen-associated molecular pattern that interacts with antigen presenting cells-impact on vaccine strategies. Vaccine. 2002;20 Suppl 4:A23-7. [PubMed: 12477424].
  • 22. Liu ZQ, Yang PC. Construction of pET-32 alpha (+) vector for protein expression and purification. N Am J Med Sci. 2012;4(12):651-5. doi: 10.4103/1947-2714.104318. [PubMed: 23272309]. [PubMed Central: PMC3530323].
  • 23. Patel JB. Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute; 2017.
  • 24. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18(3):268-81. doi: 10.1111/j.1469-0691.2011.03570.x. [PubMed: 21793988].
  • 25. Dijkshoorn L, Nemec A, Seifert H. An increasing threat in hospitals: Multidrug-resistant Acinetobacter baumannii. Nat Rev Microbiol. 2007;5(12):939-51. doi: 10.1038/nrmicro1789. [PubMed: 18007677].
  • 26. Centers for Disease Control Prevention. Overview of drug-resistant Acinetobacter infections in healthcare settings. 2010. 5 p.
  • 27. Ketter PM, Guentzel MN, Schaffer B, Herzig M, Wu X, Montgomery RK, et al. Severe Acinetobacter baumannii sepsis is associated with elevation of pentraxin 3. Infect Immun. 2014;82(9):3910-8. doi: 10.1128/IAI.01958-14. [PubMed: 25001601]. [PubMed Central: PMC4187799].
  • 28. Mohajeri P, Farahani A, Feizabadi MM, Ketabi H, Abiri R, Najafi F. Antimicrobial susceptibility profiling and genomic diversity of Acinetobacter baumannii isolates: A study in western Iran. Iran J Microbiol. 2013;5(3):195-202. [PubMed: 24475323]. [PubMed Central: PMC3895554].
  • 29. Rahbar M, Mehrgan H, Aliakbari NH. Prevalence of antibiotic-resistant Acinetobacter baumannii in a 1000-bed tertiary care hospital in Tehran, Iran. Indian J Pathol Microbiol. 2010;53(2):290-3. doi: 10.4103/0377-4929.64333. [PubMed: 20551535].
  • 30. Vahdani P, Yaghoubi T, Aminzadeh Z. Hospital acquired antibiotic-resistant acinetobacter baumannii infections in a 400-bed hospital in Tehran, Iran. Int J Prev Med. 2011;2(3):127-30. [PubMed: 21811653]. [PubMed Central: PMC3143524].
  • 31. Goudarzi H, Douraghi M, Ghalavand Z, Goudarzi M. Assessment of antibiotic resistance pattern in Acinetobacter bumannii carrying bla oxA type genes isolated from hospitalized patients. Novel Biomed. 2013;1(2):54-61. doi: 10.22037/nbm.v1i2.5093.
  • 32. Japoni-Nejad A, Sofian M, van Belkum A, Ghaznavi-Rad E. Nosocomial outbreak of extensively and pan drug-resistant Acinetobacter baumannii in tertiary hospital in central part of Iran. Jundishapur J Microbiol. 2013;6(8). doi: 10.5812/jjm.9892.
  • 33. Khan HA, Baig FK, Mehboob R. Nosocomial infections: Epidemiology, prevention, control and surveillance. Asian Pac J Trop Biomed. 2017;7(5):478-82. doi: 10.1016/j.apjtb.2017.01.019.
  • 34. Leung E, Weil DE, Raviglione M, Nakatani H; World Health Organization World Health Day Antimicrobial Resistance Technical Working Group. The WHO policy package to combat antimicrobial resistance. Bull World Health Organ. 2011;89(5):390-2. doi: 10.2471/BLT.11.088435. [PubMed: 21556308]. [PubMed Central: PMC3089396].
  • 35. Moniri R, Farahani RK, Shajari G, Shirazi MN, Ghasemi A. Molecular epidemiology of aminoglycosides resistance in acinetobacter spp. with emergence of multidrug-resistant strains. Iran J Public Health. 2010;39(2):63-8. [PubMed: 23113008]. [PubMed Central: PMC3481761].
  • 36. Peymani A, Farajnia S, Nahaei MR, Sohrabi N, Abbasi L, Ansarin K, et al. Prevalence of class 1 integron among multidrug-resistant Acinetobacter baumannii in Tabriz, northwest of Iran. Pol J Microbiol. 2012;61(1):57-60. [PubMed: 22708347].
  • 37. Gaddy JA, Tomaras AP, Actis LA. The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. Infect Immun. 2009;77(8):3150-60. doi: 10.1128/IAI.00096-09. [PubMed: 19470746]. [PubMed Central: PMC2715673].
  • 38. Singh R, Garg N, Capalash N, Kumar R, Kumar M, Sharma P. In silico analysis of Acinetobacter baumannii outer membrane protein BamA as a potential immunogen. Int J Pure Appl Sci Technol. 2014;21(2):32-9.
  • 39. Ansari H, Doosti A, Kargar M, Bijanzadeh M, Jafarinya M. Antimicrobial resistant determination and prokaryotic expression of smpA gene of Acinetobacter baumannii isolated from admitted patients. Jundishapur J Microbiol. 2017;10(11). doi: 10.5812/jjm.59370.
  • 40. Chen W. Current advances and challenges in the development of Acinetobacter vaccines. Hum Vaccin Immunother. 2015;11(10):2495-500. doi: 10.1080/21645515.2015.1052354. [PubMed: 26158773]. [PubMed Central: PMC4635877].
  • 41. Badmasti F, Ajdary S, Bouzari S, Fooladi AA, Shahcheraghi F, Siadat SD. Immunological evaluation of OMV(PagL)+Bap(1-487aa) and AbOmpA(8-346aa)+Bap(1-487aa) as vaccine candidates against Acinetobacter baumannii sepsis infection. Mol Immunol. 2015;67(2 Pt B):552-8. doi: 10.1016/j.molimm.2015.07.031. [PubMed: 26277277].
  • 42. McConnell MJ, Rumbo C, Bou G, Pachon J. Outer membrane vesicles as an acellular vaccine against Acinetobacter baumannii. Vaccine. 2011;29(34):5705-10. doi: 10.1016/j.vaccine.2011.06.001. [PubMed: 21679737].
  • 43. Bentancor LV, Routray A, Bozkurt-Guzel C, Camacho-Peiro A, Pier GB, Maira-Litran T. Evaluation of the trimeric autotransporter Ata as a vaccine candidate against Acinetobacter baumannii infections. Infect Immun. 2012;80(10):3381-8. doi: 10.1128/IAI.06096-11. [PubMed: 22825448]. [PubMed Central: PMC3457567].
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