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Application of fermentor technology in production of diphtheria toxin

AUTHORS

Ahmad Taghavi Moghadam 1 , * , Koroush Afsharpad 2

AUTHORS INFORMATION

1 Member of Scientific Board of Razi Institute, taghavi_ahmad@yahoo.com, Iran

2 Member of Scientific Board of Razi Institute, Iran

How to Cite: Taghavi Moghadam A, Afsharpad K. Application of fermentor technology in production of diphtheria toxin, Jundishapur J Microbiol. Online ahead of Print ; 1(1):24-27.

ARTICLE INFORMATION

Jundishapur Journal of Microbiology: 1 (1); 24-27
Article Type: Research Article
Received: February 1, 2008
Accepted: July 1, 2008

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Abstract

The present study shows the production of diphtheria toxoid in fermentor as compared to old static method of production. With using 45l glass fermentor which has the provision for growth of the organisms under controlled conditions with regard to temperature, pH, aeration and addition of maltose which can be automatically monitored and maintain microorganism growth toxin production and recording all of the conditions during growth. We succeeded to produce diphtheria toxin with 115 Lf (Rammon flucolation titer) within 40h time period in a minimum possible space, then the toxin was detoxified with formaldehyde, concentrated and purified and finally quality controlled and compared with toxin produced with static method. Fermentor production showed a remarkable increase in volume, titer and immunogenecity in a shorter period of time, lower contamination and minimum labor work and requirements. Therefore this technique can be industrially used for production of diphtheria Toxin (DT) and Diphtheria Pertussis Toxin (DPT) vaccines.

Keywords

Fermentor, Diphtheria, Toxin, Toxoid, DT vaccine, DPT vaccine

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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