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Genotype Analysis of Giardia lamblia Isolated From Children in Ahvaz, Southwest of Iran

AUTHORS

Elham Sadat Roointan 1 , Abdollah Rafiei 2 , * , Ali Reza Samarbaf-Zadeh 3 , Ali Akbar Shayesteh 4 , Ahmad Shamsizadeh 5 , Mahdi Pourmahdi Borujeni 6

1 Parasitology Department, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran

2 Parasitology Department, Faculty of Medicine, and Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran

3 Virology Department, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran

4 Department of Internal Medicine, Imam Khomeini Hospital, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran

5 Pediatreic Department and Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran

6 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran

How to Cite: Roointan E S, Rafiei A, Samarbaf-Zadeh A R, Shayesteh A A, Shamsizadeh A, et al. Genotype Analysis of Giardia lamblia Isolated From Children in Ahvaz, Southwest of Iran, Jundishapur J Microbiol. Online ahead of Print ; 6(3):279-83. doi: 10.5812/jjm.6443 .

ARTICLE INFORMATION

Jundishapur Journal of Microbiology: 6 (3); 279-83
Published Online: April 30, 2013
Article Type: Research Article
Received: May 22, 2012
Accepted: June 30, 2012
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Abstract

Background: Giardia lamblia is an enteric protozoan parasite, which infects human and a wide range of vertebrate hosts.

Objectives: The aim of this study was to investigate genotypes of G. lamblia from children fecal samples in Ahvaz, South West of Iran by PCR-RFLP method.

Materials and Methods: Fecal samples were collected from 58 children who were positive for G. lamblia. DNA extractions were performed by QIAamp Stool Mini Kit. DNA were evaluated by semi nested PCR-RFLP assay, targeting the glutamate dehydrogenase (gdh) gene, which was used to distinguish within and between genotypes A and B.

Results: Fifty samples (86%) were confirmed by semi-nested PCR. Genotype analysis among 50 isolates indicated 5 (10%) and 8 (16%) assemblages AII and B, respectively. Mixed Infections with both assemblages AII and B were also detected in 37 (74%) cases.

Conclusions: Current study indicated the molecular characterization of G. lamblia in southwest of Iran. Postulated sources of contamination by accidental discharge of sewage effluent and faecal contamination from animals may contribute to this high rate of mixed infection in the current study. Further studies are needed to underestand the source and route of infection better.

Keywords

Giardia lamblia, Glutamate dehydrogenase (gdh), Semi nested PCR-RFLP, Ahvaz, Iran

© 2013, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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