IF: 0.957
Cite Score:
1.46
THOMSON REUTERS - SCOPUS

Nalidixic acid susceptibility status of Salmonella enterica serovar Typhi isolates from Kolkata, India

AUTHORS

Shyamapada Mandal 1 , * , Manisha DebMandal 2 , Nishith Kumar Pal 3

AUTHORS INFORMATION

1 Department of Microbiology, Bacteriology and Serology Unit, Calcutta School of Tropical Medicine, C. R. Avenue, samtropmed@gmail.com, India

2 Department of Physiology and Biophysics, KPC Medical College and Hospital, 1F Raja S C Mallick Road, Kolkata-700 032, India

3 Department of Microbiology, Bacteriology and Serology Unit, Calcutta School of Tropical Medicine, C. R. Avenue, India

How to Cite: Mandal S, DebMandal M, Pal N. Nalidixic acid susceptibility status of Salmonella enterica serovar Typhi isolates from Kolkata, India, Jundishapur J Microbiol. Online ahead of Print ; 4(1):-.

ARTICLE INFORMATION

Jundishapur Journal of Microbiology: 4 (1)
Article Type: Research Article

Crossmark

CHEKING

READ FULL TEXT
Abstract

Introduction and objectives: Typhoid is endemic in India; multidrug resistance of Salmonella enterica serovar Typhi has been reported from Kolkata, India, but nalidixic acid (Nx) resistance has not been documented in this part of the world. The aim of this study was to determine the Nx susceptibility for S. enterica serovar Typhi isolates (1991-2001) associated with enteric fever in Kolkata, India.

Materials and methods: The clinical S. enterica serovar Typhi isolates (n=421) were subjected to Nx (the prototype quinolone, which is used for in vitro screening tests for fluoroquinolone resistance) susceptibility testing by disc diffusion and determination of minimum inhibitory concentration (MIC) values. The test results, from the two methods, were compared by scattergram analysis, and the sensitivity and specificity in determining Nx resistance by disc testing, with respect to MIC values, were calculated.

Results: The S. enterica serovar Typhi isolates were categorized into resistant, intermediate and susceptible to Nx by disc testing and MIC. The isolates showed year-wise increment of Nx MICs (0.5-256mg/ml) during 1991-2001. High sensitivity (100%) and specificity (92.96%) in determining Nx resistance by disc testing, compared to MIC values were obtained for the isolates.

Conclusion: Increasing trend of Nx resistance, as determined by two in vitro methods, among S. enterica serovar Typhi isolates was noticed; such Nx resistance may help predict decreased susceptibility to ciprofloxacin (an anti-typhoid fluoroquinolone) among emerging S. enterica serovar Typhi in our part of the globe.

Keywords

Salmonella enterica serovar Typhi Disc diffusion Minimum inhibitory concentration Nalidixic acid resistance

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Full Text

Full text is available in PDF

COMMENTS

LEAVE A COMMENT HERE: