A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran
Jundishapur Journal of Microbiology: 4 (2) Article Type: Research Article
January 1, 2011
January 20, 2011
H, Javad Zahedi
M, Darvish Moghadam
S, Hayatbakhsh Abasi
M. A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran,
Jundishapur J Microbiol.
Online ahead of Print
Introduction and objective: Metronidazole (Mtz) resistance in Helicobacter pylori has been found to be associated with mutations in rdxA, a gene encoding an oxygen insensitive NADPH nitroreductase, and enhanced by mutations in frxA a gene encoding a NAD(P)H-flavin oxidoreductase. In this study, we examined the prevalence of rdxA deletion among local isolates of H. pylori. We tested 63 Materials and methods: H. pylori isolates obtained from 191 patients referred to endoscopy unit of Afzalipour hospital in Kerman, Iran, during 2009 for their susceptibility to common anti- H. pylori antibiotics using a modified disk diffusion test. Then the metronidazole resistant and sensitive isolates were evaluated for deletion in rdxA gene by PCR methods and compared with each other. From 35 resistant Results: H. pylori isolates in this study only 22.9% (8 isolates) detected with deletion rdxA gene. No rdxA deletion was detected in sensitive isolates. According to Conclusion: rdxA deletion rates in this study, it seems that some other nitroreductases are involved in metronidazole activation or there are other metronidazole resistance mechanisms involved.
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