Jundishapur Journal of Microbiology

Published by: Kowsar

PCR Assays Based on invA Gene Amplification are not Reliable for Salmonella Detection

Carolina Resendiz-Nava 1 , Yajaira Esquivel-Hernandez 1 , Alejandro Alcaraz-Gonzalez 1 , Pilar Castaneda-Serrano 2 and Gerardo M Nava 1 , *
Authors Information
1 Department of Research and Graduate Studies in Food, Autonomous University of Queretaro, Santiago de Querétaro, Mexico
2 National Autonomous University of Mexico, Mexico City, Mexico
Article information
  • Jundishapur Journal of Microbiology: January 31, 2019, 12 (2); e68764
  • Published Online: January 16, 2019
  • Article Type: Brief Report
  • Received: March 18, 2018
  • Revised: December 29, 2018
  • Accepted: January 1, 2019
  • DOI: 10.5812/jjm.68764

To Cite: Resendiz-Nava C, Esquivel-Hernandez Y, Alcaraz-Gonzalez A, Castaneda-Serrano P, Nava G M. PCR Assays Based on invA Gene Amplification are not Reliable for Salmonella Detection, Jundishapur J Microbiol. 2019 ; 12(2):e68764. doi: 10.5812/jjm.68764.

Abstract
Copyright © 2019, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results and Discussion
5. Conclusions
Footnotes
References
  • 1. Bell RL, Jarvis KG, Ottesen AR, McFarland MA, Brown EW. Recent and emerging innovations in Salmonella detection: A food and environmental perspective. Microb Biotechnol. 2016;9(3):279-92. doi: 10.1111/1751-7915.12359. [PubMed: 27041363]. [PubMed Central: PMC4835567].
  • 2. Kasturi KN, Drgon T. Real-time PCR method for detection of Salmonella spp. in environmental samples. Appl Environ Microbiol. 2017;83(14). doi: 10.1128/AEM.00644-17. [PubMed: 28500041]. [PubMed Central: PMC5494621].
  • 3. Hu J, Huang R, Wang Y, Wei X, Wang Z, Geng Y, et al. Development of duplex PCR-ELISA for simultaneous detection of Salmonella spp. and Escherichia coli O157: H7 in food. J Microbiol Methods. 2018;154:127-33. doi: 10.1016/j.mimet.2018.10.017. [PubMed: 30393180].
  • 4. Truitt LN, Vazquez KM, Pfuntner RC, Rideout SL, Havelaar AH, Strawn LK. Microbial quality of agricultural water used in produce preharvest production on the Eastern Shore of Virginia. J Food Prot. 2018;81(10):1661-72. doi: 10.4315/0362-028X.JFP-18-185. [PubMed: 30212229].
  • 5. Rahn K, De Grandis SA, Clarke RC, McEwen SA, Galan JE, Ginocchio C, et al. Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol Cell Probes. 1992;6(4):271-9. doi: 10.1016/0890-8508(92)90002-F. [PubMed: 1528198].
  • 6. Laing CR, Whiteside MD, Gannon VPJ. Pan-genome analyses of the species Salmonella enterica, and identification of genomic markers predictive for species, subspecies, and serovar. Front Microbiol. 2017;8:1345. doi: 10.3389/fmicb.2017.01345. [PubMed: 28824552]. [PubMed Central: PMC5534482].
  • 7. Malorny B, Hoorfar J, Bunge C, Helmuth R. Multicenter validation of the analytical accuracy of Salmonella PCR: Towards an international standard. Appl Environ Microbiol. 2003;69(1):290-6. doi: 10.1128/AEM.69.1.290-296.2003. [PubMed: 12514007]. [PubMed Central: PMC152403].
  • 8. [No Authors Listed]. Google Scholar search using terms: Salmonella + PCR + invA + detection. 2018. Available from: https://scholar.google.com.
  • 9. Arnold T, Scholz HC, Marg H, Rosler U, Hensel A. Impact of invA-PCR and culture detection methods on occurrence and survival of salmonella in the flesh, internal organs and lymphoid tissues of experimentally infected pigs. J Vet Med B Infect Dis Vet Public Health. 2004;51(10):459-63. doi: 10.1111/j.1439-0450.2004.00808.x. [PubMed: 15606871].
  • 10. Scholz HC, Arnold T, Marg H, Rosler U, Hensel A. Improvement of an invA-based PCR for the specific detection of Salmonella typhimurium in organs of pigs. Berl Munch Tierarztl Wochenschr. 2001;114(9-10):401-3. doi: 10.31274/safepork-180809-1193. [PubMed: 11570189].
  • 11. Liu T, Liljebjelke K, Bartlett E, Hofacre C, Sanchez S, Maurer JJ. Application of nested polymerase chain reaction to detection of Salmonella in poultry environment. J Food Prot. 2002;65(8):1227-32. doi: 10.4315/0362-028X-65.8.1227. [PubMed: 12182472].
  • 12. Cheng CM, Lin W, Van KT, Phan L, Tran NN, Farmer D. Rapid detection of Salmonella in foods using real-time PCR. J Food Prot. 2008;71(12):2436-41. doi: 10.4315/0362-028X-71.12.2436. [PubMed: 19256088].
  • 13. Vazquez-Novelle MD, Pazos AJ, Abad M, Sanchez JL, Perez-Paralle ML. Eight-hour PCR-based procedure for the detection of Salmonella in raw oysters. FEMS Microbiol Lett. 2005;243(1):279-83. doi: 10.1016/j.femsle.2004.12.016. [PubMed: 15668030].
  • 14. Cocolin L, Manzano M, Cantoni C, Comi G. Use of polymerase chain reaction and restriction enzyme analysis to directly detect and identify Salmonella typhimurium in food. J Appl Microbiol. 1998;85(4):673-7. doi: 10.1111/j.1365-2672.1998.00575.x. [PubMed: 9812379].
  • 15. Lin CK, Tsen HY. Use of two 16S DNA targeted oligonucleotides as PCR primers for the specific detection of Salmonella in foods. J Appl Bacteriol. 1996;80(6):659-66. doi: 10.1111/j.1365-2672.1996.tb03271.x. [PubMed: 8698667].
  • 16. Trkov M, Avgustin G. An improved 16S rRNA based PCR method for the specific detection of Salmonella enterica. Int J Food Microbiol. 2003;80(1):67-75. doi: 10.1016/S0168-1605(02)00138-1. [PubMed: 12430773].
  • 17. Kim HJ, Park SH, Kim HY. Comparison of Salmonella enterica serovar Typhimurium LT2 and non-LT2 salmonella genomic sequences, and genotyping of salmonellae by using PCR. Appl Environ Microbiol. 2006;72(9):6142-51. doi: 10.1128/AEM.00138-06. [PubMed: 16957240]. [PubMed Central: PMC1563604].
  • 18. Malorny B, Paccassoni E, Fach P, Bunge C, Martin A, Helmuth R. Diagnostic real-time PCR for detection of Salmonella in food. Appl Environ Microbiol. 2004;70(12):7046-52. doi: 10.1128/AEM.70.12.7046-7052.2004. [PubMed: 15574899]. [PubMed Central: PMC535175].
  • 19. Heymans R, Vila A, van Heerwaarden CAM, Jansen CCC, Castelijn GAA, van der Voort M, et al. Rapid detection and differentiation of Salmonella species, Salmonella Typhimurium and Salmonella Enteritidis by multiplex quantitative PCR. PLoS One. 2018;13(10). e0206316. doi: 10.1371/journal.pone.0206316. [PubMed: 30359449]. [PubMed Central: PMC6201931].
  • 20. Bai J, Trinetta V, Shi X, Noll LW, Magossi G, Zheng W, et al. A multiplex real-time PCR assay, based on invA and pagC genes, for the detection and quantification of Salmonella enterica from cattle lymph nodes. J Microbiol Methods. 2018;148:110-6. doi: 10.1016/j.mimet.2018.03.019. [PubMed: 29621581].
  • 21. Lin CK, Hung CL, Hsu SC, Tsai CC, Tsen HY. An improved PCR primer pair based on 16S rDNA for the specific detection of Salmonella serovars in food samples. J Food Prot. 2004;67(7):1335-43. doi: 10.4315/0362-028X-67.7.1335. [PubMed: 15270483].
  • 22. Ceuppens S, De Coninck D, Bottledoorn N, Van Nieuwerburgh F, Uyttendaele M. Microbial community profiling of fresh basil and pitfalls in taxonomic assignment of enterobacterial pathogenic species based upon 16S rRNA amplicon sequencing. Int J Food Microbiol. 2017;257:148-56. doi: 10.1016/j.ijfoodmicro.2017.06.016. [PubMed: 28666129].
  • 23. Park SH, Kim HJ, Cho WH, Kim JH, Oh MH, Kim SH, et al. Identification of Salmonella enterica subspecies I, Salmonella enterica serovars Typhimurium, Enteritidis and Typhi using multiplex PCR. FEMS Microbiol Lett. 2009;301(1):137-46. doi: 10.1111/j.1574-6968.2009.01809.x. [PubMed: 19843307].
  • 24. Kloska F, Casteel M, Kump FW, Klein G. Implementation of a risk-orientated hygiene analysis for the control of Salmonella JAVA in the broiler production. Curr Microbiol. 2017;74(3):356-64. doi: 10.1007/s00284-017-1199-9. [PubMed: 28138784].
  • 25. Sebastiani C, Curcio L, Ciullo M, Cruciani D, Crotti S, Pesca C, et al. A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants. J Microbiol Methods. 2018;148:12-7. doi: 10.1016/j.mimet.2018.03.009. [PubMed: 29574004].
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