Jundishapur Journal of Microbiology

Published by: Kowsar

Characterization of Toxins and Colonization Factors of Enterotoxigenic Escherichia coli Isolates from Children with Acute Diarrhea in Abuja, Nigeria

Casmir Ifeanyichukwu Cajetan Ifeanyi 1 , * , Nkiruka Florence Ikeneche 2 , Bassey Enya Bassey 3 , Nazek Al-Gallas 4 , Akpa Alexander Casmir 5 and Isu Rosemary Nnennaya 6
Authors Information
1 Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Abuja, P.M.B 117, Abuja, Nigeria
2 Department of Medical Microbiology, College of Health Science, University of Abuja, P.M.B 117, Abuja, Nigeria
3 World Health Organization Office, Plot 1620, Yusuf Maitama Sule Street, Off Yakubu Gowon Crescent Asokoro, Abuja
4 Laboratoire de Controle, des Eaux et Denrees Alimentaires, Institut Pasteur de Tunis, 13 Place Pasteur, BP 74, Tunis 1002, Belvedere, Tunisie
5 Department of Medical Biotechnology, National Biotechnology Development Agency, Abuja
6 Department of Biological Science, Faculty of Science, University of Abuja, P.M.B 117, Abuja, Nigeria
Article information
  • Jundishapur Journal of Microbiology: January 2018, 11 (1); e64269
  • Published Online: December 2, 2017
  • Article Type: Research Article
  • Received: February 15, 2016
  • Revised: January 11, 2017
  • Accepted: August 12, 2017
  • DOI: 10.5812/jjm.64269

To Cite: Ifeanyichukwu Cajetan Ifeanyi C, Ikeneche N F, Bassey B E, Al-Gallas N, Alexander Casmir A, et al. Characterization of Toxins and Colonization Factors of Enterotoxigenic Escherichia coli Isolates from Children with Acute Diarrhea in Abuja, Nigeria, Jundishapur J Microbiol. 2018 ; 11(1):e64269. doi: 10.5812/jjm.64269.

Abstract
Copyright © 2017, Jundishapur Journal of Microbiology. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
6. Conclusion
Acknowledgements
Footnotes
References
  • 1. Gonzales L, Sanchez S, Zambrana S, Iniguez V, Wiklund G, Svennerholm AM, et al. Molecular characterization of enterotoxigenic Escherichia coli isolates recovered from children with diarrhea during a 4-year period (2007 to 2010) in Bolivia. J Clin Microbiol. 2013;51(4):1219-25. doi: 10.1128/JCM.02971-12. [PubMed: 23390275].
  • 2. Qadri F, Svennerholm AM, Faruque AS, Sack RB. Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clin Microbiol Rev. 2005;18(3):465-83. doi: 10.1128/CMR.18.3.465-483.2005. [PubMed: 16020685].
  • 3. Ahmed T, Bhuiyan TR, Zaman K, Sinclair D, Qadri F. Vaccines for preventing enterotoxigenic Escherichia coli (ETEC) diarrhoea. Cochrane Database Syst Rev. 2013;(7). CD009029. doi: 10.1002/14651858.CD009029.pub2. [PubMed: 23828581].
  • 4. Croxen MA, Finlay BB. Molecular mechanisms of Escherichia coli pathogenicity. Nat Rev Microbiol. 2010;8(1):26-38. doi: 10.1038/nrmicro2265. [PubMed: 19966814].
  • 5. Gaastra W, Svennerholm AM. Colonization factors of human enterotoxigenic Escherichia coli (ETEC). Trends Microbiol. 1996;4(11):444-52. [PubMed: 8950814].
  • 6. Walker RI, Steele D, Aguado T, Ad Hoc ETEC. Analysis of strategies to successfully vaccinate infants in developing countries against enterotoxigenic E. coli (ETEC) disease. Vaccine. 2007;25(14):2545-66. doi: 10.1016/j.vaccine.2006.12.028. [PubMed: 17224212].
  • 7. Johnson TJ, Nolan LK. Pathogenomics of the virulence plasmids of Escherichia coli. Microbiol Mol Biol Rev. 2009;73(4):750-74. doi: 10.1128/MMBR.00015-09. [PubMed: 19946140].
  • 8. Rivera FP, Ochoa TJ, Maves RC, Bernal M, Medina AM, Meza R, et al. Genotypic and phenotypic characterization of enterotoxigenic Escherichia coli strains isolated from Peruvian children. J Clin Microbiol. 2010;48(9):3198-203. doi: 10.1128/JCM.00644-10. [PubMed: 20631096].
  • 9. Isidean SD, Riddle MS, Savarino SJ, Porter CK. A systematic review of ETEC epidemiology focusing on colonization factor and toxin expression. Vaccine. 2011;29(37):6167-78. doi: 10.1016/j.vaccine.2011.06.084. [PubMed: 21723899].
  • 10. Shaheen HI, Abdel Messih IA, Klena JD, Mansour A, El-Wakkeel Z, Wierzba TF, et al. Phenotypic and genotypic analysis of enterotoxigenic Escherichia coli in samples obtained from Egyptian children presenting to referral hospitals. J Clin Microbiol. 2009;47(1):189-97. doi: 10.1128/JCM.01282-08. [PubMed: 18971368].
  • 11. Okeke IN, Lamikanra A, Steinruck H, Kaper JB. Characterization of Escherichia coli strains from cases of childhood diarrhea in provincial southwestern Nigeria. J Clin Microbiol. 2000;38(1):7-12. [PubMed: 10618054].
  • 12. Leiva B, Lebbad M, Winiecka-Krusnell J, Altamirano I, Tellez A, Linder E. Overdiagnosis of Entamoeba histolytica and Entamoeba dispar in Nicaragua: a microscopic, triage parasite panel and PCR study. Arch Med Res. 2006;37(4):529-34. doi: 10.1016/j.arcmed.2005.10.009. [PubMed: 16624654].
  • 13. Ifeanyi CI, Ikeneche NF, Bassey BE, Al-Gallas N, Ben Aissa R, Boudabous A. Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria. J Infect Dev Ctries. 2015;9(2):165-74. doi: 10.3855/jidc.5528. [PubMed: 25699491].
  • 14. Svennerholm AM, Holmgren J. Identification of Escherichia coli heat-labile enterotoxin by means of a ganglioside immunosorbent assay (G M1-ELISA) procedure. Current Microbiol. 1978;1(1):19-23.
  • 15. Svennerholm AM, Wikstrom M, Lindblad M, Holmgren J. Monoclonal antibodies against Escherichia coli heat-stable toxin (STa) and their use in a diagnostic ST ganglioside GM1-enzyme-linked immunosorbent assay. J Clin Microbiol. 1986;24(4):585-90. [PubMed: 2429984].
  • 16. Sjoling A, Wiklund G, Savarino SJ, Cohen DI, Svennerholm AM. Comparative analyses of phenotypic and genotypic methods for detection of enterotoxigenic Escherichia coli toxins and colonization factors. J Clin Microbiol. 2007;45(10):3295-301. doi: 10.1128/JCM.00471-07. [PubMed: 17687011].
  • 17. Evans DG, Evans DJ, Tjoa W. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infect Immun. 1977;18(2):330-7. [PubMed: 336541].
  • 18. Al-Gallas N, Abbassi SM, Hassan AB, Aissa RB. Genotypic and phenotypic profiles of enterotoxigenic Escherichia coli associated with acute diarrhea in Tunis, Tunisia. Curr Microbiol. 2007;55(1):47-55. doi: 10.1007/s00284-006-0572-x. [PubMed: 17554469].
  • 19. Tornieporth NG, John J, Salgado K, de Jesus P, Latham E, Melo MC, et al. Differentiation of pathogenic Escherichia coli strains in Brazilian children by PCR. J Clin Microbiol. 1995;33(5):1371-4. [PubMed: 7615758].
  • 20. Pradel N, Livrelli V, De Champs C, Palcoux JB, Reynaud A, Scheutz F, et al. Prevalence and characterization of Shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one-year prospective study in France. J Clin Microbiol. 2000;38(3):1023-31. [PubMed: 10698990].
  • 21. Beaudry M, Zhu C, Fairbrother JM, Harel J. Genotypic and phenotypic characterization of Escherichia coli isolates from dogs manifesting attaching and effacing lesions. J Clin Microbiol. 1996;34(1):144-8. [PubMed: 8748291].
  • 22. Bastian SN, Carle I, Grimont F. Comparison of 14 PCR systems for the detection and subtyping of stx genes in Shiga-toxin-producing Escherichia coli. Res Microbiol. 1998;149(7):457-72. [PubMed: 9766198].
  • 23. Monteiro-Neto V, Campos LC, Ferreira AJ, Gomes TA, Trabulsi LR. Virulence properties of Escherichia coli O111:H12 strains. FEMS Microbiol Lett. 1997;146(1):123-8. [PubMed: 8997716].
  • 24. McConnell MM, Chart H, Field AM, Hibberd M, Rowe B. Characterization of a putative colonization factor (PCFO166) of enterotoxigenic Escherichia coli of serogroup O166. J Gen Microbiol. 1989;135(5):1135-44. doi: 10.1099/00221287-135-5-1135. [PubMed: 2576034].
  • 25. Lopez-Vidal Y, Svennerholm AM. Monoclonal antibodies against the different subcomponents of colonization factor antigen II of enterotoxigenic Escherichia coli. J Clin Microbiol. 1990;28(9):1906-12. [PubMed: 1699969].
  • 26. Viboud GI, Binsztein N, Svennerholm AM. Characterization of monoclonal antibodies against putative colonization factors of enterotoxigenic Escherichia coli and their use in an epidemiological study. J Clin Microbiol. 1993;31(3):558-64. [PubMed: 8096215].
  • 27. Qadri F, Das SK, Faruque AS, Fuchs GJ, Albert MJ, Sack RB, et al. Prevalence of toxin types and colonization factors in enterotoxigenic Escherichia coli isolated during a 2-year period from diarrheal patients in Bangladesh. J Clin Microbiol. 2000;38(1):27-31. [PubMed: 10618058].
  • 28. Evans DJ, Evans DG, DuPont HL. Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose. Infect Immun. 1979;23(2):336-46. [PubMed: 370014].
  • 29. Ørskov F, Ørskov I. 2 Serotyping of Escherichia coli. Method Microbiol. 1984;14:43-112.
  • 30. Guinee PA, Agterberg CM, Jansen WH. Escherichia coli O antigen typing by means of a mechanized microtechnique. Appl Microbiol. 1972;24(1):127-31. [PubMed: 4560465].
  • 31. Nimmich W, Straube E, Naumann G, Budde E. Detection of rough Escherichia coli K1 strains using K1 specific phages. Fed Eur Microbiol Soc Lett. 1981;10(4):353-6.
  • 32. Vargas M, Gascon J, Casals C, Schellenberg D, Urassa H, Kahigwa E, et al. Etiology of diarrhea in children less than five years of age in Ifakara, Tanzania. Am J Trop Med Hyg. 2004;70(5):536-9. [PubMed: 15155987].
  • 33. Wayne PA. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 16th Informational Suppl. Wayne, PA; 2006.
  • 34. Peruski LJ, Kay BA, El-Yazeed RA, El-Etr SH, Cravioto A, Wierzba TF, et al. Phenotypic diversity of enterotoxigenic Escherichia coli strains from a community-based study of pediatric diarrhea in periurban Egypt. J Clin Microbiol. 1999;37(9):2974-8. [PubMed: 10449484].
  • 35. Oyofo BA, Subekti DS, Svennerholm AM, Machpud NN, Tjaniadi P, Komalarini TS, et al. Toxins and colonization factor antigens of enterotoxigenic Escherichia coli among residents of Jakarta, Indonesia. Am J Trop Med Hyg. 2001;65(2):120-4. [PubMed: 11508385].
  • 36. Rodas C, Klena JD, Nicklasson M, Iniguez V, Sjoling A. Clonal relatedness of enterotoxigenic Escherichia coli (ETEC) strains expressing LT and CS17 isolated from children with diarrhoea in La Paz, Bolivia. PLoS One. 2011;6(11). e18313. doi: 10.1371/journal.pone.0018313. [PubMed: 22140423].
  • 37. Steinsland H, Valentiner-Branth P, Grewal HM, Gaastra W, Molbak KK, Sommerfelt H. Development and evaluation of genotypic assays for the detection and characterization of enterotoxigenic Escherichia coli. Diagn Microbiol Infect Dis. 2003;45(2):97-105. [PubMed: 12614980].
  • 38. Merson MH, Orskov F, Orskov I, Sack RB, Huq I, Koster FT. Relationship between enterotoxin production and serotype in enterotoxigenic Escherichia coli. Infect Immun. 1979;23(2):325-9. [PubMed: 370013].
  • 39. Binsztein N, Jouve MJ, Viboud GI, Lopez Moral L, Rivas M, Orskov I, et al. Colonization factors of enterotoxigenic Escherichia coli isolated from children with diarrhea in Argentina. J Clin Microbiol. 1991;29(9):1893-8. [PubMed: 1774313].
  • 40. Iyer L, Vadivelu J, Parasakthi N. Colonisation factors amongst clinical isolates of enterotoxigenic Escherichia coli. Singapore Med J. 1995;36(5):495-7. [PubMed: 8882532].
  • 41. McKenzie R, Bourgeois AL, Frech SA, Flyer DC, Bloom A, Kazempour K, et al. Transcutaneous immunization with the heat-labile toxin (LT) of enterotoxigenic Escherichia coli (ETEC): protective efficacy in a double-blind, placebo-controlled challenge study. Vaccine. 2007;25(18):3684-91. doi: 10.1016/j.vaccine.2007.01.043. [PubMed: 17313998].
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