Jundishapur Journal of Microbiology

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Diversity Determination of CTX-M1 Producing Klebsiella pneumoniae Using Multilocus Variable-Number Tandem Repeat Analysis, Semnan, Iran

Shiva Mirkalantari 1 , 2 , 3 and Ali Jazayeri Moghadas 1 , *
Authors Information
1 Bacteriology and Virology Department, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, IR Iran
2 Microbiology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran
3 Institute of Immunology and Infectious Disease, Iran University of Medical Sciences, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: July 2018, 11 (7); e63131
  • Published Online: July 7, 2018
  • Article Type: Research Article
  • Received: October 25, 2017
  • Revised: May 20, 2018
  • Accepted: May 25, 2018
  • DOI: 10.5812/jjm.63131

To Cite: Mirkalantari S, Jazayeri Moghadas A. Diversity Determination of CTX-M1 Producing Klebsiella pneumoniae Using Multilocus Variable-Number Tandem Repeat Analysis, Semnan, Iran, Jundishapur J Microbiol. 2018 ; 11(7):e63131. doi: 10.5812/jjm.63131.

Copyright © 2018, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
6. Conclusions
  • 1. Fang CT, Lai SY, Yi WC, Hsueh PR, Liu KL, Chang SC. Klebsiella pneumoniae genotype k1: An emerging pathogen that causes septic ocular or central nervous system complications from pyogenic liver abscess. Clin Infect Dis. 2007;45(3):284-93. doi: 10.1086/519262.
  • 2. Gupta A, Ampofo K, Rubenstein D, Saiman L. Extended spectrum beta lactamase-producing Klebsiella pneumoniae infections: a review of the literature. J Perinatol. 2003;23(6):439-43. doi: 10.1038/sj.jp.7210973. [PubMed: 13679928].
  • 3. Kang CI, Kim SH, Park WB, Lee KD, Kim HB, Kim EC, et al. Bloodstream infections due to extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae: risk factors for mortality and treatment outcome, with special emphasis on antimicrobial therapy. Antimicrob Agents Chemother. 2004;48(12):4574-81. doi: 10.1128/AAC.48.12.4574-4581.2004. [PubMed: 15561828]. [PubMed Central: PMC529180].
  • 4. Kaftandzieva A, Trajkovska-Dokic E, Kotevska V, Cekovska Z, Jankoska G. Genotypes of ESBL producing Escherichia coli and Klebsiella pneumoniae in relation to resistance to antimicrobial drugs. Pril (Makedon Akad Nauk Umet Odd Med Nauki). 2014;35(2):31-8. [PubMed: 25500673].
  • 5. Wang Q, Li B, Tsang AK, Yi Y, Woo PC, Liu CH. Genotypic analysis of Klebsiella pneumoniae isolates in a Beijing Hospital reveals high genetic diversity and clonal population structure of drug-resistant isolates. PLoS One. 2013;8(2). e57091. doi: 10.1371/journal.pone.0057091. [PubMed: 23437318]. [PubMed Central: PMC3578803].
  • 6. Chagas TP, Alves RM, Vallim DC, Seki LM, Campos LC, Asensi MD. Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil. Braz J Infect Dis. 2011;15(5):420-5. [PubMed: 22230847].
  • 7. Rawat D, Nair D. Extended-spectrum beta-lactamases in Gram Negative Bacteria. J Glob Infect Dis. 2010;2(3):263-74. doi: 10.4103/0974-777X.68531. [PubMed: 20927289]. [PubMed Central: PMC2946684].
  • 8. Castanheira M, Mendes RE, Rhomberg PR, Jones RN. Rapid emergence of blaCTX-M among Enterobacteriaceae in U.S. Medical Centers: molecular evaluation from the MYSTIC Program (2007). Microb Drug Resist. 2008;14(3):211-6. doi: 10.1089/mdr.2008.0827. [PubMed: 18707552].
  • 9. Tabar MM, Mirkalantari S, Amoli RI. Detection of ctx-M gene in ESBL-producing E. coli strains isolated from urinary tract infection in Semnan, Iran. Electron Physician. 2016;8(7):2686-90. doi: 10.19082/2686. [PubMed: 27648198]. [PubMed Central: PMC5014510].
  • 10. Rossolini GM, D'Andrea MM, Mugnaioli C. The spread of CTX-M-type extended-spectrum beta-lactamases. Clin Microbiol Infect. 2008;14 Suppl 1:33-41. doi: 10.1111/j.1469-0691.2007.01867.x. [PubMed: 18154526].
  • 11. Naseer U, Olsson-Liljequist BE, Woodford N, Dhanji H, Canton R, Sundsfjord A, et al. Multi-locus variable number of tandem repeat analysis for rapid and accurate typing of virulent multidrug resistant Escherichia coli clones. PLoS One. 2012;7(7). e41232. doi: 10.1371/journal.pone.0041232. [PubMed: 22859970]. [PubMed Central: PMC3407997].
  • 12. Li W, Raoult D, Fournier PE. Bacterial strain typing in the genomic era. FEMS Microbiol Rev. 2009;33(5):892-916. doi: 10.1111/j.1574-6976.2009.00182.x. [PubMed: 19453749].
  • 13. Krawczyk B, Samet A, Czarniak E, Szczapa J, Kur J. Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: control of an outbreak using a new ADSRRS technique. Pol J Microbiol. 2005;54(2):105-10. [PubMed: 16209103].
  • 14. Al-Marzooq F, Mohd Yusof MY, Tay ST. Molecular Analysis of Antibiotic Resistance Determinants and Plasmids in Malaysian Isolates of Multidrug Resistant Klebsiella pneumoniae. PLoS One. 2015;10(7). e0133654. doi: 10.1371/journal.pone.0133654. [PubMed: 26203651]. [PubMed Central: PMC4512681].
  • 15. Tille P. Bailey & Scott's Diagnostic Microbiology-E-Book. Elsevier Health Sciences; 2015.
  • 16. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing, 24th Informational Supplement (M100-S24). Wayne; 2014.
  • 17. Kuske CR, Banton KL, Adorada DL, Stark PC, Hill KK, Jackson PJ. Small-Scale DNA Sample Preparation Method for Field PCR Detection of Microbial Cells and Spores in Soil. Appl Environ Microbiol. 1998;64(7):2463-72. [PubMed: 9647816]. [PubMed Central: PMC106412].
  • 18. Mena A, Plasencia V, Garcia L, Hidalgo O, Ayestaran JI, Alberti S, et al. Characterization of a large outbreak by CTX-M-1-producing Klebsiella pneumoniae and mechanisms leading to in vivo carbapenem resistance development. J Clin Microbiol. 2006;44(8):2831-7. doi: 10.1128/JCM.00418-06. [PubMed: 16891499]. [PubMed Central: PMC1594597].
  • 19. Turton JF, Perry C, Elgohari S, Hampton CV. PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets. J Med Microbiol. 2010;59(Pt 5):541-7. doi: 10.1099/jmm.0.015198-0. [PubMed: 20110386].
  • 20. Bina M, Pournajaf A, Mirkalantari S, Talebi M, Irajian G. Detection of the Klebsiella pneumoniae carbapenemase (KPC) in K. pneumoniae Isolated from the Clinical Samples by the Phenotypic and Genotypic Methods. Iran J Pathol. 2015;10(3):199-205. [PubMed: 26351485]. [PubMed Central: PMC4539771].
  • 21. Fernandez-Canigia L, Dowzicky MJ. Susceptibility of important Gram-negative pathogens to tigecycline and other antibiotics in Latin America between 2004 and 2010. Ann Clin Microbiol Antimicrob. 2012;11:29. doi: 10.1186/1476-0711-11-29. [PubMed: 23088592]. [PubMed Central: PMC3499165].
  • 22. Blomberg B, Jureen R, Manji KP, Tamim BS, Mwakagile DS, Urassa WK, et al. High rate of fatal cases of pediatric septicemia caused by gram-negative bacteria with extended-spectrum beta-lactamases in Dar es Salaam, Tanzania. J Clin Microbiol. 2005;43(2):745-9. doi: 10.1128/JCM.43.2.745-749.2005. [PubMed: 15695674]. [PubMed Central: PMC548071].
  • 23. Tuon FF, Kruger M, Terreri M, Penteado-Filho SR, Gortz L. Klebsiella ESBL bacteremia-mortality and risk factors. Braz J Infect Dis. 2011;15(6):594-8. [PubMed: 22218521].
  • 24. Kim YK, Pai H, Lee HJ, Park SE, Choi EH, Kim J, et al. Bloodstream infections by extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in children: epidemiology and clinical outcome. Antimicrob Agents Chemother. 2002;46(5):1481-91. [PubMed: 11959586]. [PubMed Central: PMC127143].
  • 25. Singh AK, Jain S, Kumar D, Singh RP, Bhatt H. Antimicrobial susceptibility pattern of extended-spectrum beta- lactamase producing Klebsiella pneumoniae clinical isolates in an Indian tertiary hospital. J Res Pharm Pract. 2015;4(3):153-9. doi: 10.4103/2279-042X.162363. [PubMed: 26312255]. [PubMed Central: PMC4548435].
  • 26. Nepal K, Pant ND, Neupane B, Belbase A, Baidhya R, Shrestha RK, et al. Extended spectrum beta-lactamase and metallo beta-lactamase production among Escherichia coli and Klebsiella pneumoniae isolated from different clinical samples in a tertiary care hospital in Kathmandu, Nepal. Ann Clin Microbiol Antimicrob. 2017;16(1):62. doi: 10.1186/s12941-017-0236-7. [PubMed: 28927454]. [PubMed Central: PMC5605977].
  • 27. Paterson DL, Bonomo RA. Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev. 2005;18(4):657-86. doi: 10.1128/CMR.18.4.657-686.2005. [PubMed: 16223952]. [PubMed Central: PMC1265908].
  • 28. Chong Y, Ito Y, Kamimura T. Genetic evolution and clinical impact in extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae. Infect Gene Evol. 2011;11(7):1499-504. doi: 10.1016/j.meegid.2011.06.001.
  • 29. Iraz M, Ozad Duzgun A, Sandalli C, Doymaz MZ, Akkoyunlu Y, Saral A, et al. Distribution of beta-lactamase genes among carbapenem-resistant Klebsiella pneumoniae strains isolated from patients in Turkey. Ann Lab Med. 2015;35(6):595-601. doi: 10.3343/alm.2015.35.6.595. [PubMed: 26354347]. [PubMed Central: PMC4579103].
  • 30. Valenzuela de Silva EM, Mantilla Anaya JR, Reguero Reza MT, Gonzalez Mejia EB, Pulido Manrique IY, Dario Llerena I, et al. Detection of CTX-M-1, CTX-M-15, and CTX-M-2 in clinical isolates of Enterobacteriaceae in Bogota, Colombia. J Clin Microbiol. 2006;44(5):1919-20. doi: 10.1128/JCM.44.5.1919-1920.2006. [PubMed: 16672442]. [PubMed Central: PMC1479192].
  • 31. Rossolini GM, D'Andrea MM, Mugnaioli C. The spread of CTX-M-type extended-spectrum β-lactamases. Clin Microbiol Infect. 2008;14:33-41. doi: 10.1111/j.1469-0691.2007.01867.x.
  • 32. Canton R, Gonzalez-Alba JM, Galan JC. CTX-M Enzymes: Origin and Diffusion. Front Microbiol. 2012;3:110. doi: 10.3389/fmicb.2012.00110. [PubMed: 22485109]. [PubMed Central: PMC3316993].
  • 33. D'Andrea MM, Arena F, Pallecchi L, Rossolini GM. CTX-M-type beta-lactamases: a successful story of antibiotic resistance. Int J Med Microbiol. 2013;303(6-7):305-17. doi: 10.1016/j.ijmm.2013.02.008. [PubMed: 23490927].
  • 34. Karami N, Helldal L, Welinder-Olsson C, Ahren C, Moore ER. Sub-typing of extended-spectrum-beta-lactamase-producing isolates from a nosocomial outbreak: application of a 10-loci generic Escherichia coli multi-locus variable number tandem repeat analysis. PLoS One. 2013;8(12). e83030. doi: 10.1371/journal.pone.0083030. [PubMed: 24391735]. [PubMed Central: PMC3877011].

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