Jundishapur Journal of Microbiology

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Virulence Genes and Phenotypic Evaluation of the Antibiotic Resistance of Vero Toxin Producing Escherichia coli Recovered From Milk, Meat, and Vegetables

Zohreh Mashak 1 , *
Author Information
1 Department of Food Hygiene, Karaj Branch, Islamic Azad University, Karaj, Iran
Article information
  • Jundishapur Journal of Microbiology: May 2018, 11 (5); e62288
  • Published Online: May 7, 2018
  • Article Type: Research Article
  • Received: October 23, 2017
  • Revised: May 3, 2018
  • Accepted: April 22, 2018
  • DOI: 10.5812/jjm.62288

To Cite: Mashak Z. Virulence Genes and Phenotypic Evaluation of the Antibiotic Resistance of Vero Toxin Producing Escherichia coli Recovered From Milk, Meat, and Vegetables, Jundishapur J Microbiol. 2018 ; 11(5):e62288. doi: 10.5812/jjm.62288.

Abstract
Copyright © 2018, Jundishapur Journal of Microbiology. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
6. Conclusions
Acknowledgements
Footnotes
References
  • 1. Gould LH, Walsh KA, Vieira AR, Herman K, Williams IT, Hall AJ, et al. Surveillance for foodborne disease outbreaks—United States, 1998–2008. MMWR Surveill Summ. 2013 Jun 28;62(2):1-34. [PubMed: 23804024].
  • 2. Havelaar AH, Kirk MD, Torgerson PR, Gibb HJ, Hald T, Lake RJ, et al. World Health Organization Global Estimates and Regional Comparisons of the Burden of Foodborne Disease in 2010. PLoS Med. 2015;12(12). e1001923. doi: 10.1371/journal.pmed.1001923. [PubMed: 26633896].
  • 3. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, et al. Foodborne Illness Acquired in the United States—Major Pathogens. Emerg Infect Dis. 2011;17(1):7-15. doi: 10.3201/eid1701.P11101.
  • 4. Momtaz H, Karimian A, Madani M, Safarpoor Dehkordi F, Ranjbar R, Sarshar M, et al. Uropathogenic Escherichia coli in Iran: Serogroup distributions, virulence factors and antimicrobial resistance properties. Ann Clin Microbiol Antimicrob. 2013;12(1):8. doi: 10.1186/1476-0711-12-8.
  • 5. Momtaz H, Farzan R, Rahimi E, Safarpoor Dehkordi F, Souod N. Molecular Characterization of Shiga Toxin-ProducingEscherichia coliIsolated from Ruminant and Donkey Raw Milk Samples and Traditional Dairy Products in Iran. Sci World J. 2012;2012:1-13. doi: 10.1100/2012/231342.
  • 6. Dehkordi F, Yazdani F, Mozafari J, Valizadeh Y. Virulence factors, serogroups and antimicrobial resistance properties of Escherichia coli strains in fermented dairy products. BMC Res Notes. 2014;7(1):217. doi: 10.1186/1756-0500-7-217.
  • 7. Bai X, Zhang W, Tang X, Xin Y, Xu Y, Sun H, et al. Shiga Toxin-Producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China. Front Microbiol. 2016;7. doi: 10.3389/fmicb.2016.00375.
  • 8. Dhaka P, Vijay D, Vergis J, Negi M, Kumar M, Mohan V, et al. Genetic diversity and antibiogram profile of diarrhoeagenic Escherichia coli pathotypes isolated from human, animal, foods and associated environmental sources. Infect Ecol Epidemiol. 2016;6:31055. doi: 10.3402/iee.v6.31055. [PubMed: 27197617].
  • 9. Wang J, Stanford K, McAllister TA, Johnson RP, Chen J, Hou H, et al. Biofilm Formation, Virulence Gene Profiles, and Antimicrobial Resistance of Nine Serogroups of Non-O157 Shiga Toxin-Producing Escherichia coli. Foodborne Pathog Dis. 2016;13(6):316-24. doi: 10.1089/fpd.2015.2099. [PubMed: 27023165].
  • 10. Farshad S, Ranijbar R, Japoni A, Hosseini M, Anvarinejad M, Mohammadzadegan R. Microbial susceptibility, virulence factors, and plasmid profiles of uropathogenic Escherichia coli strains isolated from children in Jahrom, Iran. Arch Iran Med. 2012 May;15(5):312-6.
  • 11. Amezquita-Lopez BA, Quinones B, Soto-Beltran M, Lee BG, Yambao JC, Lugo-Melchor OY, et al. Antimicrobial resistance profiles of Shiga toxin-producing Escherichia coli O157 and Non-O157 recovered from domestic farm animals in rural communities in Northwestern Mexico. Antimicrob Resist Infect Control. 2016;5:1. doi: 10.1186/s13756-015-0100-5. [PubMed: 26734130].
  • 12. Abdi S. Frequency of bla TEM, bla SHV, bla CTX-M, and qnrA among Escherichia coli isolated from urinary tract infection. Arch Clin Infect Dis. 2014;9(1):1-5.
  • 13. Woo PC, Cheung EY, Leung K, Yuen K. Identification by 16S ribosomal RNA gene sequencing of an Enterobacteriaceae species with ambiguous biochemical profile from a renal transplant recipient. Diagn Microbiol Infect Dis. 2001;39(2):85-93. [PubMed: 11248520].
  • 14. Momtaz H, Dehkordi F, Hosseini M, Sarshar M, Heidari M. Serogroups, virulence genes and antibiotic resistance in Shiga toxin-producing Escherichia coli isolated from diarrheic and non-diarrheic pediatric patients in Iran. Gut Pathogens. 2013;5(1):39. doi: 10.1186/1757-4749-5-39.
  • 15. Hemmatinezhad B, Khamesipour F, Mohammadi M, Safarpoor Dehkordi F, Mashak Z. Microbiological Investigation of O-Serogroups, Virulence Factors and Antimicrobial Resistance Properties of Shiga Toxin-ProducingEscherichia ColiIsolated from Ostrich, Turkey and Quail Meats. J Food Safety. 2015;35(4):491-500. doi: 10.1111/jfs.12199.
  • 16. CLSI. Clinical and Laboratory Standards Institute (CLSI) performance standards for antimicrobial disk diffusion susceptibility tests approved standard. 2012.
  • 17. Yahaghi E, Khamesipour F, Mashayekhi F, Safarpoor Dehkordi F, Sakhaei MH, Masoudimanesh M, et al. Helicobacter pyloriin Vegetables and Salads: Genotyping and Antimicrobial Resistance Properties. BioMed Res Int. 2014;2014:1-11. doi: 10.1155/2014/757941.
  • 18. Wachtel MR, Whitehand LC, Mandrell RE. Association of Escherichia coli O157:H7 with preharvest leaf lettuce upon exposure to contaminated irrigation water. J Food Prot. 2002;65(1):18-25. [PubMed: 11808792].
  • 19. Solomon EB, Yaron S, Matthews KR. Transmission of Escherichia coli O157: H7 from contaminated manure and irrigation water to lettuce plant tissue and its subsequent internalization. Appl Environ Microbiol. 2002 Jan;68(1):397-400. [PubMed: 11772650].
  • 20. Solomon EB, Pang HJ, Matthews KR. Persistence of Escherichia coli O157:H7 on lettuce plants following spray irrigation with contaminated water. J Food Prot. 2003;66(12):2198-202. [PubMed: 14672213].
  • 21. Momtaz H, Safarpoor Dehkordi F, Taktaz T, Rezvani A, Yarali S. Shiga Toxin-ProducingEscherichia coliIsolated from Bovine Mastitic Milk: Serogroups, Virulence Factors, and Antibiotic Resistance Properties. Sci World J. 2012;2012:1-9. doi: 10.1100/2012/618709.
  • 22. Franz E, Klerks MM, De Vos OJ, Termorshuizen AJ, van Bruggen AHC. Prevalence of Shiga Toxin-Producing Escherichia coli stx1, stx2, eaeA, and rfbE Genes and Survival of E. coli O157:H7 in Manure from Organic and Low-Input Conventional Dairy Farms. Appl Environ Microbiol. 2007;73(7):2180-90. doi: 10.1128/aem.01950-06.
  • 23. Zhou D, Jay-Russell MT, Hake AF, Bengson Y, Thiptara A, Nguyen T. Prevalence and Characterization of Escherichia coli and Salmonella Strains Isolated from Stray Dog and Coyote Feces in a Major Leafy Greens Production Region at the United States-Mexico Border. PLoS ONE. 2014;9(11). e113433. doi: 10.1371/journal.pone.0113433.
  • 24. Kabiru L, Bello M, Kabir J, Grande L, Morabito S. Detection of Pathogenic Escherichia coli in Samples Collected at an Abattoir in Zaria, Nigeria and at Different Points in the Surrounding Environment. Int J Environ Res Public Health. 2015;12(1):679-91. doi: 10.3390/ijerph120100679.
  • 25. Dormanesh B, Siroosbakhat S, Karimi Goudarzi P, Afsharkhas L. RETRACTED ARTICLE: Shiga toxigenic Escherichia coli in Iranian pediatric patients with and without diarrhea: O-serogroups, virulence factors and antimicrobial resistance properties. Iran Red Crescent Med J. 2015;17(10). doi: 10.5812/ircmj.29706.
  • 26. Dormanesh B, Safarpoor Dehkordi F, Momtaz H, Mirnejad R, Hoseini MJ, Yahaghi E, et al. Virulence Factors and O-Serogroups Profiles of Uropathogenic Escherichia Coli Isolated from Iranian Pediatric Patients. Iran Red Crescent Med J. 2014;16(2). doi: 10.5812/ircmj.14627.
  • 27. Rasheed MU, Thajuddin N, Ahamed P, Teklemariam Z, Jamil K. Antimicrobial drug resistance in strains of Escherichia coli isolated from food sources. Revista do Instituto de Medicina Tropical de Sao Paulo. 2014;56(4):341-6. doi: 10.1590/s0036-46652014000400012.
  • 28. Colello R, Etcheverria AI, Di Conza JA, Gutkind GO, Padola NL. Antibiotic resistance and integrons in Shiga toxin-producing Escherichia coli (STEC). Braz J Microbiol. 2015;46(1):1-5. doi: 10.1590/S1517-838246120130698. [PubMed: 26221083].
  • 29. Miles TD, McLaughlin W, Brown PD. Antimicrobial resistance of Escherichia coli isolates from broiler chickens and humans. BMC Vet Res. 2006;2:7. doi: 10.1186/1746-6148-2-7. [PubMed: 16460561].
  • 30. Kim S, Woo GJ. Prevalence and characterization of antimicrobial-resistant Escherichia coli isolated from conventional and organic vegetables. Foodborne Pathog Dis. 2014;11(10):815-21. doi: 10.1089/fpd.2014.1771. [PubMed: 25140978].
  • 31. Dehkordi Hasanpour A, Khaji L, Shahreza MH, Mashak Z, Dehkordi FS, Safaee Y, et al. One-year prevalence of antimicrobial susceptibility pattern of methicillin-resistant Staphylococcus aureus recovered from raw meat. Trop Biomed. 2017;34(2):396-404.
  • 32. Ranjbar R, Masoudimanesh M, Dehkordi FS, Jonaidi-Jafari N, Rahimi E. Shiga (Vero)-toxin producing Escherichia coli isolated from the hospital foods; virulence factors, o-serogroups and antimicrobial resistance properties. Antimicrob Resist Infect Control. 2017;6:4. doi: 10.1186/s13756-016-0163-y. [PubMed: 28074125].
  • 33. Safarpoor Dehkordi F, Parsaei P, Saberian S, Moshkelani S, Hajshafiei P, Hoseini SR, et al. Prevalence study of theileria annulata by comparison of four diagnostic techniques in Southwest Iran. Bulgarian J Vet Med. 2012;15(2).
  • 34. Atapoor S, Safarpoor Dehkordi F, Rahimi E. Detection of Helicobacter pylori in Various Types of Vegetables and Salads. Jundishapur J Microbiol. 2014;7(5). e10013. doi: 10.5812/jjm.10013. [PubMed: 25147709].
  • 35. Safarpoor Dehkordi F, Barati S, Momtaz H, Hosseini Ahari SN, Nejat Dehkordi S. Comparison of Shedding, and Antibiotic Resistance Properties of Listeria monocytogenes Isolated From Milk, Feces, Urine, and Vaginal Secretion of Bovine, Ovine, Caprine, Buffalo, and Camel Species in Iran. Jundishapur J Microbiol. 2013. doi: 10.5812/jjm.6616.
  • 36. Rahimi E, Sepehri S, Safarpoor Dehkordi F, Shaygan S, Momtaz H. Prevalence of Yersinia Species in Traditional and Commercial Dairy Products in Isfahan Province, Iran. Jundishapur J Microbiol. 2014;7(4). doi: 10.5812/jjm.9249.
  • 37. Momtaz H, Davood Rahimian M, Safarpoor Dehkordi F. Identification and characterization of Yersinia enterocolitica isolated from raw chicken meat based on molecular and biological techniques. J Appl Poult Res. 2013;22(1):137-45. doi: 10.3382/japr.2012-00549.
  • 38. Momtaz H, Dehkordi FS, Rahimi E, Asgarifar A. Detection of Escherichia coli, Salmonella species, and Vibrio cholerae in tap water and bottled drinking water in Isfahan, Iran. BMC Public Health. 2013;13:556. doi: 10.1186/1471-2458-13-556. [PubMed: 23742181].
  • 39. Momtaz H, Safarpoor Dehkordi F, Rahimi E, Ezadi H, Arab R. Incidence of Shiga toxin-producing Escherichia coli serogroups in ruminant's meat. Meat Sci. 2013;95(2):381-8. doi: 10.1016/j.meatsci.2013.04.051. [PubMed: 23747633].
  • 40. Shahrani M, Dehkordi FS, Momtaz H. Characterization of Escherichia coli virulence genes, pathotypes and antibiotic resistance properties in diarrheic calves in Iran. Biol Res. 2014;47:28. doi: 10.1186/0717-6287-47-28. [PubMed: 25052999].
  • 41. Safarpoor Dehkordi F, Haghighi N, Momtaz H, Rafsanjani M, Momeni M. Conventional vs real-time PCR for detection of bovine herpes virus type 1 in aborted bovine, buffalo and camel foetuses. Bulgarian J Vet Med. 2013;16(2):102-11.
  • 42. Mousavi S, Dehkordi F, Rahimi E. Virulence factors and antibiotic resistance of Helicobacter pylori isolated from raw milk and unpasteurized dairy products in Iran. J Venomous Animals Toxins Trop Dis. 2014;20(1):51. doi: 10.1186/1678-9199-20-51.
  • 43. Momtaz H, Dehkordi FS, Rahimi E, Asgarifar A, Momeni M. Virulence genes and antimicrobial resistance profiles of Staphylococcus aureus isolated from chicken meat in Isfahan province, Iran. J Appl Poult Res. 2013;22(4):913-21. doi: 10.3382/japr.2012-00673.
  • 44. Safarpoor Dehkordi F, Tirgir F, Valizadeh Y. Effects of Guajol((R)) ointment synthesized from medicinal smoke condensate of jennet feces on burn wound healing on Wistar rat. Vet Res Forum. 2017;8(3):215-21. [PubMed: 29085609].
  • 45. Madahi H, Rostami F, Rahimi E, Safarpoor Dehkordi F. Prevalence of Enterotoxigenic Staphylococcus aureus Isolated From Chicken Nugget in Iran. Jundishapur J Microbiol. 2014;7(7). doi: 10.5812/jjm.10237.
  • 46. Safarpoor Dehkordi F, Khamesipour F, Momeni M. Brucella abortus and Brucella melitensis in Iranian Bovine and Buffalo Semen Samples: The First Clinical Trial on Seasonal, Senile and Geographical Distribution Using Culture, Conventional and Real-time Polymerase Chain Reaction Assays. Kafkas Universitesi Veteriner Fakultesi Dergisi. 2014;20(6):821-8. doi: 10.9775/kvfd.2014.10827.
  • 47. Safarpoor Dehkordi F, Gandomi H, Basti AA, Misaghi A, Rahimi E. Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food. Antimicrob Resist Infect Control. 2017;6:104. doi: 10.1186/s13756-017-0257-1. [PubMed: 29034091].
  • 48. Ghorbani F, Gheisari E, Safarpoor Dehkordi F. Genotyping of vacA alleles of Helicobacter pylori strains recovered from some Iranian food items. Trop J Pharm Res. 2016;15(8):1631. doi: 10.4314/tjpr.v15i8.5.
  • 49. Dehkordi FS, Borujeni MR, Rahimi E, Abdizadeh R. Detection of Toxoplasma gondii in raw caprine, ovine, buffalo, bovine, and camel milk using cell cultivation, cat bioassay, capture ELISA, and PCR methods in Iran. Foodborne Pathog Dis. 2013;10(2):120-5. doi: 10.1089/fpd.2012.1311. [PubMed: 23441913].

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