Jundishapur Journal of Microbiology

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Rapid Detection of Rifampicin- and Isoniazid-Resistant Mycobacterium tuberculosis Using Real-Time PCR

Leyla Sahebi 1 , Khalil Ansarin 1 , * , Amir Monfaredan 2 , Safar Farajnia 3 , Seiran Nili 4 and Majid Khalili 5
Authors Information
1 PhD of Molecular Epidemiology, Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran
2 Department of Hematology, Faculty of Medicine, Tabriz Branch, Islamic Azad University, Tabriz, IR Iran
3 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran
4 PhD Student of Epidemiology, Health Deputy, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
5 Medical Philosophy and History Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: October 2016, 9 (10); e60173
  • Published Online: April 16, 2016
  • Article Type: Research Article
  • Received: April 10, 2015
  • Revised: June 28, 2015
  • Accepted: July 14, 2015
  • DOI: 10.5812/jjm.29147

To Cite: Sahebi L, Ansarin K, Monfaredan A, Farajnia S, Nili S, et al. Rapid Detection of Rifampicin- and Isoniazid-Resistant Mycobacterium tuberculosis Using Real-Time PCR, Jundishapur J Microbiol. 2016 ; 9(10):e60173. doi: 10.5812/jjm.29147.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Luo T, Jiang L, Sun W, Fu G, Mei J, Gao Q. Multiplex Real-Time PCR Melting Curve Assay To Detect Drug-Resistant Mutations of Mycobacterium tuberculosis. J Clin Microbiol. 2011; 49(9): 3132-8[DOI]
  • 2. Kocagoz T, Saribas Z, Alp A. Rapid Determination of Rifampin Resistance in Clinical Isolates of Mycobacterium tuberculosis by Real-Time PCR. J. Clin Microbiol. 2005; 43(12): 6015-619[DOI]
  • 3. Velayati AA, Masjedi MR, Farnia P, Tabarsi P, Ghanavi J, ZiaZarifi AH, et al. Emergence of New Forms of Totally Drug-Resistant Tuberculosis Bacilli. Chest. 2009; 136(2): 420-5[DOI]
  • 4. Ardito F, Posteraro B, Sanguinetti M, Zanetti S, Fadda G. Evaluation of BACTEC Mycobacteria Growth Indicator Tube (MGIT 960) Automated System for Drug Susceptibility Testing of Mycobacterium tuberculosis. Journal of Clinical Microbiology. 2001; 39(12): 4440-4[DOI]
  • 5. Sekiguchi JI, Miyoshi-Akiyama T, Augustynowicz-Kopec E, Zwolska Z, Kirikae F, Toyota E, et al. Detection of Multidrug Resistance in Mycobacterium tuberculosis. J Clin Microbiol. 2006; 45(1): 179-92[DOI]
  • 6. Riska PF, Jacobs J, Alland D. Molecular determinants of drug resistance in tuberculosis. Int J Tuberc Lung Dis. 2000; 4(2 Suppl 1)-10[PubMed]
  • 7. Park WG, Bishai WR, Chaisson RE, Dorman SE. Performance of the Microscopic Observation Drug Susceptibility Assay in Drug Susceptibility Testing for Mycobacterium tuberculosis. J Clin Microbiol. 2002; 40(12): 4750-2[DOI]
  • 8. Lin SYG, Probert W, Lo M, Desmond E. Rapid Detection of Isoniazid and Rifampin Resistance Mutations in Mycobacterium tuberculosis Complex from Cultures or Smear-Positive Sputa by Use of Molecular Beacons. J Clin Microbiol. 2004; 42(9): 4204-8[DOI]
  • 9. Helb D, Jones M, Story E, Boehme C, Wallace E, Ho K, et al. Rapid Detection ofMycobacterium tuberculosisand Rifampin Resistance by Use of On-Demand, Near-Patient Technology. J Clin Microbiol. 2010; 48(1): 229-37[DOI]
  • 10. Garcia de Viedma D, del Sol Diaz Infantes M, Lasala F, Chaves F, Alcala L, Bouza E. New Real-Time PCR Able To Detect in a Single Tube Multiple Rifampin Resistance Mutations and High-Level Isoniazid Resistance Mutations in Mycobacterium tuberculosis. J Clin Microbiol. 2002; 40(3): 988-95[DOI]
  • 11. Anti-tuberculosis drug resistance in the world: third global report. 2004;
  • 12. Rafi A, Moaddab SR. Subspecies tests in: Principles of mycobacteriology. 2004; : 95-114
  • 13. van Soolingen D, Qian L, de Haas PE, Douglas JT, Traore H, Portaels F, et al. Predominance of a single genotype of Mycobacterium tuberculosis in countries of east Asia. J Clin Microbiol. 1995; 33(12): 3234-8[PubMed]
  • 14. Marin M, de Viedma DG, Ruiz-Serrano MJ, Bouza E. Rapid Direct Detection of Multiple Rifampin and Isoniazid Resistance Mutations in Mycobacterium tuberculosis in Respiratory Samples by Real-Time PCR. Antimicrob Agents Chemother. 2004; 48(11): 4293[DOI]
  • 15. Yang Z, Durmaz R, Yang D, Gunal S, Zhang L, Foxman B, et al. Simultaneous detection of isoniazid, rifampin, and ethambutol resistance of Mycobacterium tuberculosis by a single multiplex allele-specific polymerase chain reaction (PCR) assay. Diagn Microbiol Infect Dis. 2005; 53(3): 201-8[DOI]
  • 16. Dooley KE, Lahlou O, Ghali I, Knudsen J, Elmessaoudi MD, Cherkaoui I, et al. Risk factors for tuberculosis treatment failure, default, or relapse and outcomes of retreatment in Morocco. BMC Public Health. 2011; 11(1): 140[DOI]
  • 17. Sahebi L, Ansarin K, Maryam S, Monfaredan A, Sabbgh Jadid H. The factors associated with tuberculosis recurrence in the northwest and west of iran. Malays J Med Sci. 2014; 21(6): 27-35[PubMed]
  • 18. Mohajeri P, Sadri H, Farahani A, Norozi B, Atashi S. Frequency of Mutations Associated with Rifampicin Resistance inMycobacterium tuberculosisStrains Isolated from Patients in West of Iran. Microb Drug Resis. 2015; 21(3): 315-9[DOI]
  • 19. Torres MJ, Criado A, Palomares JC, Aznar J. Use of real-time PCR and fluorimetry for rapid detection of rifampin and isoniazid resistance-associated mutations in Mycobacterium tuberculosis. J Clin Microbiol. 2000; 38(9): 3194-9[PubMed]
  • 20. Garcia de Viedma D. Rapid detection of resistance in Mycobacterium tuberculosis: a review discussing molecular approaches. Iran J Clin Infect Disease. 2003; 9(5): 349-59[DOI]
  • 21. Feizabadi MM, Haeili M, Fooladi AI, Bostanabad SZ, Sarokhalil DD, Siavoshi F. Rapid screening of rpoB and katG mutations in Mycobacterium tuberculosis isolates by high-resolution melting curve analysis. Indian Journal of Medical Microbiology. 2014; 32(4): 398[DOI]
  • 22. Sheikholslami MF, Farnia P, Tabarsi P, Merza MA, Amiri MVP, Mohammadi F, et al. Comparison of polymerase chain reaction single-strand conformation polymorphism with DNA sequencing to detect drug resistance of mycobacterium tuberculosis isolates. Iran J Clin Infect Disease. 2011; 6(2): 66-70
  • 23. Weis SE, Slocum PC, Blais FX, King B, Nunn M, Matney GB, et al. The effect of directly observed therapy on the rates of drug resistance and relapse in tuberculosis. N Engl J Med. 1994; 330(17): 1179-84[DOI][PubMed]
  • 24. Johnson R. Understanding the mechanisms of drug resistance in enhancing rapid molecular detection of drug resistance in Mycobacterium tuberculosis. 2007;
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