Jundishapur Journal of Microbiology

Published by: Kowsar

PCR Based Detection of Phase Variable Genes in Pakistani Based Clinical Helicobacter pylori Strains

Sajjad Ahmad 1 , * , Faisal Ahmad 1 , Faiz ur Rahman 1 , Salman Khan 1 , Waheed Murad 2 , Imran Mughal 3 , Amjad ur Rahman 4 , Fida Muhammad Khan 5 , Imad Khan 6 and Hajra Ahmad 7
Authors Information
1 Department of Microbiology, Faculty of Biological Sciences, Quaid-i-azam University, Islamabad, Pakistan
2 Department of Microbiology and Molecular Genetics (MMG), University of Punjab, Lahore, Pakistan
3 Center of Biotechnology and Microbiology (COBAM), University of Peshawar, Peshawar, Pakistan
4 DHQ Teaching Hospital, KDA Kohat, Khyber Pukhtoon Khwa, Pakistan
5 Bioresource Research Center (BRC), Islamabad, Pakistan
6 College of Veterinary Science and Animal Husbandry, Abdul Wali khan University, Mardan, Pakistan
7 Department of Medicine, Khyber Teaching Hospital (KTH), Peshawar, Pakistan
Article information
  • Jundishapur Journal of Microbiology: July 2016, 9 (7); e60097
  • Published Online: July 3, 2016
  • Article Type: Research Article
  • Received: July 26, 2015
  • Revised: September 30, 2015
  • Accepted: June 11, 2016
  • DOI: 10.5812/jjm.31824

To Cite: Ahmad S, Ahmad F, Rahman F U, Khan S, Murad W, et al. PCR Based Detection of Phase Variable Genes in Pakistani Based Clinical Helicobacter pylori Strains, Jundishapur J Microbiol. 2016 ; 9(7):e60097. doi: 10.5812/jjm.31824.

Abstract
Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
Acknowledgements
Footnote
References
  • 1. Blaser MJ. Who are we? Indigenous microbes and the ecology of human diseases. EMBO Rep. 2006; 7(10): 956-60[DOI][PubMed]
  • 2. Kusters JG, van Vliet AH, Kuipers EJ. Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev. 2006; 19(3): 449-90[DOI][PubMed]
  • 3. Kansau I, Raymond J, Bingen E, Courcoux P, Kalach N, Bergeret M, et al. Genotyping of Helicobacter pylori isolates by sequencing of PCR products and comparison with the RAPD technique. Res Microbiol. 1996; 147(8): 661-9[PubMed]
  • 4. Aspholm-Hurtig M, Dailide G, Lahmann M, Kalia A, Ilver D, Roche N, et al. Functional adaptation of BabA, the H. pylori ABO blood group antigen binding adhesin. Science. 2004; 305(5683): 519-22[DOI][PubMed]
  • 5. van der Woude MW. Phase variation: how to create and coordinate population diversity. Curr Opin Microbiol. 2011; 14(2): 205-11[DOI][PubMed]
  • 6. Tomb JF, White O, Kerlavage AR, Clayton RA, Sutton GG, Fleischmann RD, et al. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature. 1997; 388(6642): 539-47[DOI][PubMed]
  • 7. Saunders NJ, Peden JF, Hood DW, Moxon ER. Simple sequence repeats in the Helicobacter pylori genome. Mol Microbiol. 1998; 27(6): 1091-8[PubMed]
  • 8. Alm RA, Ling LS, Moir DT, King BL, Brown ED, Doig PC, et al. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature. 1999; 397(6715): 176-80[DOI][PubMed]
  • 9. Tannaes T, Dekker N, Bukholm G, Bijlsma JJ, Appelmelk BJ. Phase variation in the Helicobacter pylori phospholipase A gene and its role in acid adaptation. Infect Immun. 2001; 69(12): 7334-40[DOI][PubMed]
  • 10. Salaun L, Linz B, Suerbaum S, Saunders NJ. The diversity within an expanded and redefined repertoire of phase-variable genes in Helicobacter pylori. Microbiology. 2004; 150: 817-30[DOI][PubMed]
  • 11. Salaun L, Ayraud S, Saunders NJ. Phase variation mediated niche adaptation during prolonged experimental murine infection with Helicobacter pylori. Microbiology. 2005; 151: 917-23[DOI][PubMed]
  • 12. Christopher LC, Harry LTM. DNA methylation blocking factor. 1997; 28
  • 13. Enroth H, Nyren O, Engstrand L. One stomach--one strain: does Helicobacter pylori strain variation influence disease outcome? Dig Dis Sci. 1999; 44(1): 102-7[PubMed]
  • 14. Rasheed F, Khan A, Farooqui A, Ahmad T, Manzoor H, Akhtar SS. Emerging antimicrobial resistance in helicobacter pylori strains isolated from gastric disease patients in Karachi, Pakistan. Pak J Sci . 2011; 54(2): 59-63
  • 15. Rasheed F, Ahmad T, Zaidi NA, Bilal R. Frequency of Helicobacter pylori infection among symptomatic patients of Rawalpindi and Islamabad, Pakistan. J Coll Physicians Surg Pak. 2011; 21(3): 193-4[PubMed]
  • 16. Andres S, Skoglund A, Nilsson C, Krabbe M, Bjorkholm B, Engstrand L. Type I restriction-modification loci reveal high allelic diversity in clinical Helicobacter pylori isolates. Helicobacter. 2010; 15(2): 114-25[DOI][PubMed]
  • 17. Gauntlett JC, Nilsson H, Fulurija A, Marshall BJ, Benghezal M. Phase-variable restriction/modification systems are required for Helicobacter pylori colonization. Gut Pathog. 2014; 6(35)[DOI]
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