Jundishapur Journal of Microbiology

Published by: Kowsar

Evaluation of Clarithromycin Resistance Among Iranian Helicobacter pylori Isolates by E-Test and Real-Time Polymerase Chain Reaction Methods

Mojdeh Hakemi Vala 1 , * , Shirin Eyvazi 2 , Hossein Goudarzi 1 , Hamid Reza Sarie 3 and Mehrdad Gholami 4
Authors Information
1 Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
2 Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
3 Fayazbakhsh Hospital, Tehran, IR Iran
4 Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran
Article information
  • Jundishapur Journal of Microbiology: May 2016, 9 (5); e60063
  • Published Online: March 19, 2016
  • Article Type: Research Article
  • Received: June 8, 2015
  • Revised: December 20, 2015
  • Accepted: January 24, 2016
  • DOI: 10.5812/jjm.29839

To Cite: Hakemi Vala M, Eyvazi S, Goudarzi H, Sarie H R, Gholami M. Evaluation of Clarithromycin Resistance Among Iranian Helicobacter pylori Isolates by E-Test and Real-Time Polymerase Chain Reaction Methods, Jundishapur J Microbiol. 2016 ; 9(5):e60063. doi: 10.5812/jjm.29839.

Abstract
Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Kusters JG, van Vliet AH, Kuipers EJ. Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev. 2006; 19(3): 449-90[DOI][PubMed]
  • 2. Alborzi A, Soltani J, Pourabbas B, Oboodi B, Haghighat M, Hayati M, et al. Prevalence of Helicobacter pylori infection in children (south of Iran). Diagn Microbiol Infect Dis. 2006; 54(4): 259-61[DOI][PubMed]
  • 3. Brown LM. Helicobacter pylori: epidemiology and routes of transmission. Epidemiol Rev. 2000; 22(2): 283-97[PubMed]
  • 4. McColl KE. Clinical practice. Helicobacter pylori infection. N Engl J Med. 2010; 362(17): 1597-604[DOI][PubMed]
  • 5. Gerrits MM. 2004; [PubMed]
  • 6. Gisbert JP. Rescue Therapy for Helicobacter pylori Infection 2012. Gastroenterol Res Pract. 2012; 2012: 974594[DOI][PubMed]
  • 7. Lee JH, Shin JH, Roe IH, Sohn SG, Lee JH, Kang GH, et al. Impact of clarithromycin resistance on eradication of Helicobacter pylori in infected adults. Antimicrob Agents Chemother. 2005; 49(4): 1600-3[DOI][PubMed]
  • 8. De Francesco V, Giorgio F, Hassan C, Manes G, Vannella L, Panella C, et al. Worldwide H. pylori antibiotic resistance: a systematic review. J Gastrointestin Liver Dis. 2010; 19(4): 409-14[PubMed]
  • 9. Houben MH, van de Beek D, Hensen EF, de Craen AJ, et al. A systematic review of Helicobacter pylori eradication therapy—the impact of antimicrobial resistance on eradication rates Aliment Pharmacol Ther 1999; 13(8): 1047-55[PubMed]
  • 10. Eyvazi S, Hakemi-Vala M. A Review Article on Helicobacter pylori Antibiotic Resistance Profile in Iran. Int J Trop Dis Health. 2015; 10(1): 1-12
  • 11. Yu C, Li L, Chen W, Jiao Y, Yang N, Yang E, et al. Levofloxacin susceptibility testing for Helicobacter pylori in China: comparison of E-test and disk diffusion method. Helicobacter. 2011; 16(2): 119-23[DOI][PubMed]
  • 12. Mishra KK, Srivastava S, Garg A, Ayyagari A. Antibiotic susceptibility of Helicobacter pylori clinical isolates: comparative evaluation of disk-diffusion and E-test methods. Curr Microbiol. 2006; 53(4): 329-34[DOI][PubMed]
  • 13. van Doorn LJ, Glupczynski Y, Kusters JG, Megraud F, Midolo P, Maggi-Solca N, et al. Accurate prediction of macrolide resistance in Helicobacter pylori by a PCR line probe assay for detection of mutations in the 23S rRNA gene: multicenter validation study. Antimicrob Agents Chemother. 2001; 45(5): 1500-4[DOI][PubMed]
  • 14. Morlan J, Baker J, Sinicropi D. Mutation detection by real-time PCR: a simple, robust and highly selective method. PLoS One. 2009; 4(2)[DOI][PubMed]
  • 15. Bernard PS, Wittwer CT. Real-time PCR technology for cancer diagnostics. Clin Chem. 2002; 48(8): 1178-85[PubMed]
  • 16. McCulloch E, Lucas C, Ramage G, Williams C. Improved early diagnosis of Pseudomonas aeruginosa by real-time PCR to prevent chronic colonisation in a paediatric cystic fibrosis population. J Cyst Fibros. 2011; 10(1): 21-4[DOI][PubMed]
  • 17. Oleastro M, Menard A, Santos A, Lamouliatte H, Monteiro L, Barthelemy P, et al. Real-time PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori. J Clin Microbiol. 2003; 41(1): 397-402[PubMed]
  • 18. Malekzadeh R, Mohamadnejad M, Siavoshi F, Massarrat S. Treatment of Helicobacter pylori infection in Iran: low efficacy of recommended western regimens. Arch Iranian Med. 2004; 7(1): 1-8
  • 19. Holmes DS, Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981; 114(1): 193-7[PubMed]
  • 20. Queipo-Ortuno MI, De Dios Colmenero J, Macias M, Bravo MJ, Morata P. Preparation of bacterial DNA template by boiling and effect of immunoglobulin G as an inhibitor in real-time PCR for serum samples from patients with brucellosis. Clin Vaccine Immunol. 2008; 15(2): 293-6[DOI][PubMed]
  • 21. Kanoh S, Rubin BK. Mechanisms of action and clinical application of macrolides as immunomodulatory medications. Clin Microbiol Rev. 2010; 23(3): 590-615[DOI][PubMed]
  • 22. Megraud F. The challenge of Helicobacter pylori resistance to antibiotics: the comeback of bismuth-based quadruple therapy. Therap Adv Gastroenterol. 2012; 5(2): 103-9[DOI][PubMed]
  • 23. Ogata SK, Gales AC, Kawakami E. Antimicrobial susceptibility testing for Helicobacter pylori isolates from Brazilian children and adolescents: comparing agar dilution, E-test, and disk diffusion. Braz J Microbiol. 2014; 45(4): 1439-48[PubMed]
  • 24. De Francesco V, Zullo A, Ierardi E, Giorgio F, Perna F, Hassan C, et al. Phenotypic and genotypic Helicobacter pylori clarithromycin resistance and therapeutic outcome: benefits and limits. J Antimicrob Chemother. 2010; 65(2): 327-32[DOI][PubMed]
  • 25. Bakir OS, Ozakin C, Keskin M. [Antibiotic resistance rates of Helicobacter pylori isolates and the comparison of E-test and fluorescent in situ hybridization methods for the detection of clarithromycin resistant strains]. Microbiol bulteni. 2009; 43(2): 227-34
  • 26. Agudo S, Perez-Perez G, Alarcon T, Lopez-Brea M. High prevalence of clarithromycin-resistant Helicobacter pylori strains and risk factors associated with resistance in Madrid, Spain. J Clin Microbiol. 2010; 48(10): 3703-7[DOI][PubMed]
  • 27. Lang L, Garcia F. Comparison of E-test and disk diffusion assay to evaluate resistance of Helicobacter pylori isolates to amoxicillin, clarithromycin, metronidazole and tetracycline in Costa Rica. Int J Antimicrob Agents. 2004; 24(6): 572-7[DOI][PubMed]
  • 28. Chang WL, Sheu BS, Cheng HC, Yang YJ, Yang HB, Wu JJ. Resistance to metronidazole, clarithromycin and levofloxacin of Helicobacter pylori before and after clarithromycin-based therapy in Taiwan. J Gastroenterol Hepatol. 2009; 24(7): 1230-5[DOI][PubMed]
  • 29. Gahbauer AM, Gonzales ML, Guglielmo BJ. Patterns of antibacterial use and impact of age, race/ethnicity, and geographic region on antibacterial use in an outpatient medicaid cohort. Pharmacotherapy. 2014; 34(7): 677-85[DOI][PubMed]
  • 30. Sirous M, Mehrabadi JF, Daryani NE, Eshraghi S, Hajikhani S, Shirazi MH. Prevalence of antimicrobial resistance in Helicobacter pylori isolates from Iran. Afr J Biotechnol . 2013; 9(36)
  • 31. Fallahi GH, Maleknejad S. Helicobacter pylori culture and antimicrobial resistance in Iran. Indian J Pediatr. 2007; 74(2): 127-30[PubMed]
  • 32. Farshad S, Alborzi A, Japoni A, Ranjbar R, Hosseini Asl K, Badiee P, et al. Antimicrobial susceptibility of Helicobacter pylori strains isolated from patients in Shiraz, Southern Iran. World J Gastroenterol. 2010; 16(45): 5746-51[PubMed]
  • 33. Rafeey M, Ghotaslou R, Nikvash S, Hafez AA. Primary resistance in Helicobacter pylori isolated in children from Iran. J Infect Chemother. 2007; 13(5): 291-5[DOI][PubMed]
  • 34. Ghotaslou R, Milani M, Akhi MT, Hejazi MS, Nahaei MR, Hasani A, et al. Relationship between drug resistance and cagA Gene in Helicobacter pylori. Jundishapur J Microbiol. 2013; 6(10)[DOI]
  • 35. Talebi Bezmin Abadi A, Mobarez AM, Taghvaei T, Wolfram L. Antibiotic resistance of Helicobacter pylori in Mazandaran, North of Iran. Helicobacter. 2010; 15(6): 505-9[DOI][PubMed]
  • 36. Sadeghifard N, Seidnazari T, Ghafourian S, Soleimani M, Maleki A, Qomi MA, et al. Survey in Iran of clarithromycin resistance in Helicobacter pylori isolates by PCR-RFLP. Southeast Asian J Trop Med Public Health. 2013; 44(1): 89-95[PubMed]
  • 37. Shokrzadeh L, Alebouyeh M, Mirzaei T, Farzi N, Zali MR. Prevalence of multiple drug-resistant Helicobacter pylori strains among patients with different gastric disorders in Iran. Microb Drug Resist. 2015; 21(1): 105-10[DOI][PubMed]
  • 38. Garcia-Arata MI, Baquero F, de Rafael L, Martin de Argila C, Gisbert JP, Bermejo F, et al. Mutations in 23S rRNA in Helicobacter pylori conferring resistance to erythromycin do not always confer resistance to clarithromycin. Antimicrob Agents Chemother. 1999; 43(2): 374-6[PubMed]
  • 39. Kargar M, Baghernejad M, Doosti A, Ghorbani-Dalini S. Clarithromycin resistance and 23S rRNA mutations in Helicobacter pylori isolates in Iran. Afr J Microbiol Res. 2011; 5(8): 853-6
  • 40. Mohammadi M, Doroud D, Mohajerani N, Massarrat S. Helicobacter pylori antibiotic resistance in Iran. World J Gastroenterol. 2005; 11(38): 6009-13[PubMed]
  • 41. Kim JM, Kim JS, Kim N, Kim YJ, Kim IY, Chee YJ, et al. Gene mutations of 23S rRNA associated with clarithromycin resistance in Helicobacter pylori strains isolated from Korean patients. J Microbiol Biotechnol. 2008; 18(9): 1584-9[PubMed]
  • 42. Alarcon T, Vega AE, Domingo D, Martinez MJ, Lopez-Brea M. Clarithromycin resistance among Helicobacter pylori strains isolated from children: prevalence and study of mechanism of resistance by PCR-restriction fragment length polymorphism analysis. J Clin Microbiol. 2003; 41(1): 486-99[PubMed]
  • 43. Alvarez A, Moncayo JI, Santacruz JJ, Santacoloma M, Corredor LF, Reinosa E. Antimicrobial susceptibility and mutations involved in clarithromycin resistance in Helicobacter pylori isolates from patients in the western central region of Colombia. Antimicrob Agents Chemother. 2009; 53(9): 4022-4[DOI][PubMed]
  • 44. Naserpour Farivar T, Najafipour R, Johari P. Prevalence of clarithromycin-resistant Helicobacter pylori in patients with chronic tonsillitis by allele-specific Scorpion real-time polymerase chain reaction assay. Laryngoscope. 2013; 123(6): 1478-82[DOI][PubMed]
  • 45. Khademi F, Faghri J, Moghim S, Esfahani BN, Fazeli H, Poursina F, et al. The study of mutation in 23S rRNA resistance gene of Helicobacter pylori to clarithromycin in patients with gastrointestinal disorders in Isfahan - Iran. Adv Biomed Res. 2014; 3: 98[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments