Jundishapur Journal of Microbiology

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Identification of Candida Species Using MP65 Gene and Evaluation of the Candida albicans MP65 Gene Expression in BALB/C Mice

Farahnaz Bineshian 1 , * , Mohammad Hossien Yadegari 2 , Zohre Sharifi 3 , Mohammadreza Akbari Eidgahi 4 and Reza Nasr 4
Authors Information
1 Department of Mycology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, IR Iran
2 Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran
3 Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IR Iran
4 Department of Biotechnology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, IR Iran
Article information
  • Jundishapur Journal of Microbiology: May 2015, 8 (5); e59848
  • Published Online: May 31, 2015
  • Article Type: Research Article
  • Received: April 7, 2014
  • Revised: October 22, 2014
  • Accepted: November 4, 2014
  • DOI: 10.5812/jjm.8(5)2015.18984

To Cite: Bineshian F, Yadegari M H, Sharifi Z, Akbari Eidgahi M, Nasr R. Identification of Candida Species Using MP65 Gene and Evaluation of the Candida albicans MP65 Gene Expression in BALB/C Mice, Jundishapur J Microbiol. 2015 ; 8(5):e59848. doi: 10.5812/jjm.8(5)2015.18984.

Abstract
Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences.This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. La Valle R, Sandini S, Gomez MJ, Mondello F, Romagnoli G, Nisini R, et al. Generation of a recombinant 65-kilodalton mannoprotein, a major antigen target of cell-mediated immune response to Candida albicans. Infect Immun. 2000; 68(12): 6777-84[PubMed]
  • 2. Berzaghi R, Colombo AL, Machado AM, de Camargo ZP. New approach for diagnosis of candidemia based on detection of a 65-kilodalton antigen. Clin Vaccine Immunol. 2009; 16(11): 1538-45[DOI][PubMed]
  • 3. Gudlaugsson O, Gillespie S, Lee K, Vande Berg J, Hu J, Messer S, et al. Attributable mortality of nosocomial candidemia, revisited. Clin Infect Dis. 2003; 37(9): 1172-7[DOI][PubMed]
  • 4. Arancia S, Sandini S, Cassone A, De Bernardis F. Use of 65kDa mannoprotein gene primers in PCR methods for the identification of five medically important Candida species. Mol Cell Probes. 2009; 23(5): 218-26[DOI][PubMed]
  • 5. Pappas PG, Rex JH, Sobel JD, Filler SG, Dismukes WE, Walsh TJ, et al. Guidelines for treatment of candidiasis. Clin Infect Dis. 2004; 38(2): 161-89[DOI][PubMed]
  • 6. Kim J, Sudbery P. Candida albicans, a major human fungal pathogen. J Microbiol. 2011; 49(2): 171-7[DOI][PubMed]
  • 7. Sudbery PE. Growth of Candida albicans hyphae. Nat Rev Microbiol. 2011; 9(10): 737-48[DOI][PubMed]
  • 8. Gomez MJ, Maras B, Barca A, La Valle R, Barra D, Cassone A. Biochemical and immunological characterization of MP65, a major mannoprotein antigen of the opportunistic human pathogen Candida albicans. Infect Immun. 2000; 68(2): 694-701[PubMed]
  • 9. Gow NA, van de Veerdonk FL, Brown AJ, Netea MG. Candida albicans morphogenesis and host defence: discriminating invasion from colonization. Nat Rev Microbiol. 2011; 10(2): 112-22
  • 10. Heilmann CJ, Sorgo AG, Siliakus AR, Dekker HL, Brul S, de Koster CG, et al. Hyphal induction in the human fungal pathogen Candida albicans reveals a characteristic wall protein profile. Microbiology. 2011; 157: 2297-307[DOI][PubMed]
  • 11. Xu H, Nobile CJ, Dongari-Bagtzoglou A. Glucanase induces filamentation of the fungal pathogen Candida albicans. PLoS One. 2013; 8(5)[DOI][PubMed]
  • 12. Sorgo AG, Heilmann CJ, Brul S, de Koster CG, Klis FM. Beyond the wall: Candida albicans secret(e)s to survive. FEMS Microbiol Lett. 2013; 338(1): 10-7[DOI][PubMed]
  • 13. Sandini S, Stringaro A, Arancia S, Colone M, Mondello F, Murtas S, et al. The MP65 gene is required for cell wall integrity, adherence to epithelial cells and biofilm formation in Candida albicans. BMC Microbiol. 2011; 11: 106[DOI][PubMed]
  • 14. Sandini S, La Valle R, De Bernardis F, Macri C, Cassone A. The 65 kDa mannoprotein gene of Candida albicans encodes a putative beta-glucanase adhesin required for hyphal morphogenesis and experimental pathogenicity. Cell Microbiol. 2007; 9(5): 1223-38[DOI][PubMed]
  • 15. Malani PN, Bradley SF, Little RS, Kauffman CA. Trends in species causing fungaemia in a tertiary care medical centre over 12 years. Mycoses. 2001; 44(11-12): 446-9[PubMed]
  • 16. Xiang H, Xiong L, Liu X, Tu Z. Rapid simultaneous detection and identification of six species Candida using polymerase chain reaction and reverse line hybridization assay. J Microbiol Methods. 2007; 69(2): 282-7[DOI][PubMed]
  • 17. Stehr F, Felk A, Gacser A, Kretschmar M, Mahnss B, Neuber K, et al. Expression analysis of the Candida albicans lipase gene family during experimental infections and in patient samples. FEMS Yeast Res. 2004; 4(4-5): 401-8[PubMed]
  • 18. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29(9)[PubMed]
  • 19. Szabo EK, MacCallum DM. The contribution of mouse models to our understanding of systemic candidiasis. FEMS Microbiol Lett. 2011; 320(1): 1-8[DOI][PubMed]
  • 20. White PL, Shetty A, Barnes RA. Detection of seven Candida species using the Light-Cycler system. J Med Microbiol. 2003; 52: 229-38[PubMed]
  • 21. Arancia S, Sandini S, Cassone A, De Bernardis F, La Valle R. Construction and use of PCR primers from a 65 kDa mannoprotein gene for identification of C. albicans. Mol Cell Probes. 2004; 18(3): 171-5[DOI][PubMed]
  • 22. Arancia S, Carattoli A, La Valle R, Cassone A, De Bernardis F. Use of 65 kDa mannoprotein gene primers in Real Time PCR identification of Candida albicans in biological samples. Mol Cell Probes. 2006; 20(5): 263-8[DOI][PubMed]
  • 23. Francois JM. A simple method for quantitative determination of polysaccharides in fungal cell walls. Nat Protoc. 2006; 1(6): 2995-3000[DOI][PubMed]
  • 24. Nisini R, Romagnoli G, Gomez MJ, La Valle R, Torosantucci A, Mariotti S, et al. Antigenic properties and processing requirements of 65-kilodalton mannoprotein, a major antigen target of anti-Candida human T-cell response, as disclosed by specific human T-cell clones. Infect Immun. 2001; 69(6): 3728-36[DOI][PubMed]
  • 25. Morgunova E, Saller S, Haase I, Cushman M, Bacher A, Fischer M, et al. Lumazine synthase from Candida albicans as an anti-fungal target enzyme: structural and biochemical basis for drug design. J Biol Chem. 2007; 282(23): 17231-41[DOI][PubMed]
  • 26. Hiller E, Heine S, Brunner H, Rupp S. Candida albicans Sun41p, a putative glycosidase, is involved in morphogenesis, cell wall biogenesis, and biofilm formation. Eukaryot Cell. 2007; 6(11): 2056-65[DOI][PubMed]
  • 27. De Bernardis F, Liu H, O'Mahony R, La Valle R, Bartollino S, Sandini S, et al. Human domain antibodies against virulence traits of Candida albicans inhibit fungus adherence to vaginal epithelium and protect against experimental vaginal candidiasis. J Infect Dis. 2007; 195(1): 149-57[DOI][PubMed]
  • 28. Arancia S, Sandini S, De Bernardis F, Fortini D. Rapid, simple, and low-cost identification of Candida species using high-resolution melting analysis. Diagn Microbiol Infect Dis. 2011; 69(3): 283-5[DOI][PubMed]
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