Jundishapur Journal of Microbiology

Published by: Kowsar

Expression Profiles of TGF-β and TLR Pathways in Porphyromonas gingivalis and Prevotella intermedia Challenged Osteoblasts

Kubra Aydin 1 , Fatma Yesim Ekinci 1 and May Korachi 1 , *
Authors Information
1 Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey
Article information
  • Jundishapur Journal of Microbiology: April 2015, 8 (4); e59843
  • Published Online: April 18, 2015
  • Article Type: Research Article
  • Received: January 29, 2014
  • Revised: July 14, 2014
  • Accepted: July 25, 2014
  • DOI: 10.5812/jjm.8(4)2015.17920

To Cite: Aydin K, Ekinci F Y, Korachi M. Expression Profiles of TGF-β and TLR Pathways in Porphyromonas gingivalis and Prevotella intermedia Challenged Osteoblasts, Jundishapur J Microbiol. 2015 ; 8(4):e59843. doi: 10.5812/jjm.8(4)2015.17920.

Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences.This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Pye AD, Lockhart DE, Dawson MP, Murray CA, Smith AJ. A review of dental implants and infection. J Hosp Infect. 2009; 72(2): 104-10[DOI][PubMed]
  • 2. Chug A, Shukla S, Mahesh L, Jadwani S. Osseointegration—Molecular events at the bone–implant interface. JOMSMP. 2013; 25: 1–4
  • 3. Baker PJ. The role of immune responses in bone loss during periodontal disease. Microbes Infect. 2000; 2(10): 1181-92[PubMed]
  • 4. Heydenrijk K, Meijer HJ, van der Reijden WA, Raghoebar GM, Vissink A, Stegenga B. Microbiota around root-form endosseous implants: a review of the literature. Int J Oral Maxillofac Implants. 2002; 17(6): 829-38[PubMed]
  • 5. Amano A, Nakagawa I, Okahashi N, Hamada N. Variations of Porphyromonas gingivalis fimbriae in relation to microbial pathogenesis. J Periodontal Res. 2004; 39(2): 136-42[PubMed]
  • 6. Guan SM, Zhang M, He JJ, Wu JZ. Mitogen-activated protein kinases and phosphatidylinositol 3-kinase are involved in Prevotella intermedia-induced proinflammatory cytokines expression in human periodontal ligament cells. Biochem Biophys Res Commun. 2009; 386(3): 471-6[DOI][PubMed]
  • 7. Feng Z, Weinberg A. Role of bacteria in health and disease of periodontal tissues. Periodontol 2000. 2006; 40: 50-76[PubMed]
  • 8. Chen G, Deng C, Li YP. TGF-beta and BMP signaling in osteoblast differentiation and bone formation. Int J Biol Sci. 2012; 8(2): 272-88[DOI][PubMed]
  • 9. Casanova JL, Abel L, Quintana-Murci L. Human TLRs and IL-1Rs in host defense: natural insights from evolutionary, epidemiological, and clinical genetics. Annu Rev Immunol. 2011; 29: 447-91[PubMed]
  • 10. Tang Y, Wu X, Lei W, Pang L, Wan C, Shi Z, et al. TGF-beta1-induced migration of bone mesenchymal stem cells couples bone resorption with formation. Nat Med. 2009; 15(7): 757-65[DOI][PubMed]
  • 11. Lin X, Wu J, Xie H. Porphyromonas gingivalis minor fimbriae are required for cell-cell interactions. Infect Immun. 2006; 74(10): 6011-5[DOI][PubMed]
  • 12. Tamura M, Tokuda M, Nagaoka S, Takada H. Lipopolysaccharides of Bacteroides intermedius (Prevotella intermedia) and Bacteroides (Porphyromonas) gingivalis induce interleukin-8 gene expression in human gingival fibroblast cultures. Infect Immun. 1992; 60(11): 4932-7[PubMed]
  • 13. Kadono H, Kido J, Kataoka M, Yamauchi N, Nagata T. Inhibition of osteoblastic cell differentiation by lipopolysaccharide extract from Porphyromonas gingivalis. Infect Immun. 1999; 67(6): 2841-6[PubMed]
  • 14. Iki K, Kawahara K, Sawamura S, Arakaki R, Sakuta T, Sugiyama A, et al. A novel component different from endotoxin extracted from Prevotella intermedia ATCC 25611 activates lymphoid cells from C3H/HeJ mice and gingival fibroblasts from humans. Infect Immun. 1997; 65(11): 4531-8[PubMed]
  • 15. Parisuthiman D, Mochida Y, Duarte WR, Yamauchi M. Biglycan modulates osteoblast differentiation and matrix mineralization. J Bone Miner Res. 2005; 20(10): 1878-86[DOI][PubMed]
  • 16. Clarke B. Normal bone anatomy and physiology. Clin J Am Soc Nephrol. 2008; 3 Suppl 3-9[DOI][PubMed]
  • 17. Gerstenfeld LC, Chipman SD, Glowacki J, Lian JB. Expression of differentiated function by mineralizing cultures of chicken osteoblasts. Dev Biol. 1987; 122(1): 49-60[PubMed]
  • 18. Shin NR, Jeong EH, Choi CI, Moon HJ, Kwon CH, Chu IS, et al. Overexpression of Snail is associated with lymph node metastasis and poor prognosis in patients with gastric cancer. BMC Cancer. 2012; 12: 521[DOI][PubMed]
  • 19. Kim YJ, Lee MH, Wozney JM, Cho JY, Ryoo HM. Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2. J Biol Chem. 2004; 279(49): 50773-80[DOI][PubMed]
  • 20. Corps AN, Robinson AH, Movin T, Costa ML, Hazleman BL, Riley GP. Increased expression of aggrecan and biglycan mRNA in Achilles tendinopathy. Rheumatology (Oxford). 2006; 45(3): 291-4[DOI][PubMed]
  • 21. Zhang W, Swearingen EB, Ju J, Rigney T, Tribble GD. Porphyromonas gingivalis invades osteoblasts and inhibits bone formation. Microbes Infect. 2010; 12(11): 838-45[DOI][PubMed]
  • 22. Pelt P, Zimmermann B, Ulbrich N, Bernimoulin JP. Effects of lipopolysaccharide extracted from Prevotella intermedia on bone formation and on the release of osteolytic mediators by fetal mouse osteoblasts in vitro. Arch Oral Biol. 2002; 47(12): 859-66[PubMed]
  • 23. Moser M, Binder O, Wu Y, Aitsebaomo J, Ren R, Bode C, et al. BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation. Mol Cell Biol. 2003; 23(16): 5664-79[PubMed]
  • 24. Singhatanadgit W, Salih V, Olsen I. RNA interference of the BMPR-IB gene blocks BMP-2-induced osteogenic gene expression in human bone cells. Cell Biol Int. 2008; 32(11): 1362-70[DOI][PubMed]
  • 25. Garcia-Martin A, Reyes-Garcia R, Avila-Rubio V, Munoz-Torres M. Osteocalcin: a link between bone homeostasis and energy metabolism. Endocrinol Nutr. 2013; 60(5): 260-3[DOI][PubMed]
  • 26. Rodrigues AM, Caetano-Lopes J, Vale AC, Vidal B, Lopes A, Aleixo I, et al. Low osteocalcin/collagen type I bone gene expression ratio is associated with hip fragility fractures. Bone. 2012; 51(6): 981-9[DOI][PubMed]
  • 27. Maehata YM, Lee MCL, Hata RIH. Roles of Collagen Molecules in Growth and Differentiation of Human Osteoblasts. Oral Biosci J. 2009; 51: 72–80
  • 28. Ducy P, Desbois C, Boyce B, Pinero G, Story B, Dunstan C, et al. Increased bone formation in osteocalcin-deficient mice. Nature. 1996; 382(6590): 448-52[DOI][PubMed]
  • 29. Chen W, Fu X, Sheng Z. Review of current progress in the structure and function of Smad proteins. Chin Med J (Engl). 2002; 115(3): 446-50[PubMed]
  • 30. Hellingman CA, Davidson EN, Koevoet W, Vitters EL, van den Berg WB, van Osch GJ, et al. Smad signaling determines chondrogenic differentiation of bone-marrow-derived mesenchymal stem cells: inhibition of Smad1/5/8P prevents terminal differentiation and calcification. Tissue Eng Part A. 2011; 17(7-8): 1157-67[DOI][PubMed]
  • 31. Filvaroff E, Erlebacher A, Ye J, Gitelman SE, Lotz J, Heillman M, et al. Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass. Development. 1999; 126(19): 4267-79[PubMed]
  • 32. Jovcic G, Ivanovic Z, Biljanovic-Paunovic L, Bugarski D, Stosic-Grujicic S, Milenkovic P. The effect of IL-1 receptor antagonist on the proliferation of hematopoietic progenitor cells in regenerating bone marrow. Leukemia. 1996; 10(3): 564-9[PubMed]
  • 33. Wilkinson MG, Millar JB. Control of the eukaryotic cell cycle by MAP kinase signaling pathways. FASEB J. 2000; 14(14): 2147-57[DOI][PubMed]
  • 34. Mogi M, Ozeki N, Nakamura H, Togari A. Dual roles for NF-kappaB activation in osteoblastic cells by serum deprivation: osteoblastic apoptosis and cell-cycle arrest. Bone. 2004; 35(2): 507-16[DOI][PubMed]
  • 35. Beutler B. Inferences, questions and possibilities in Toll-like receptor signalling. Nature. 2004; 430(6996): 257-63[DOI][PubMed]
  • 36. Sun Y, Shu R, Li CL, Zhang MZ. Gram-negative periodontal bacteria induce the activation of Toll-like receptors 2 and 4, and cytokine production in human periodontal ligament cells. J Periodontol. 2010; 81(10): 1488-96[DOI][PubMed]
  • 37. Sartori R, Li F, Kirkwood KL. MAP kinase phosphatase-1 protects against inflammatory bone loss. J Dent Res. 2009; 88(12): 1125-30[DOI][PubMed]
  • 38. Talbot S, Totemeyer S, Yamamoto M, Akira S, Hughes K, Gray D, et al. Toll-like receptor 4 signalling through MyD88 is essential to control Salmonella enterica serovar typhimurium infection, but not for the initiation of bacterial clearance. Immunology. 2009; 128(4): 472-83[PubMed]
  • 39. Zhang G, Ghosh S. Negative regulation of toll-like receptor-mediated signaling by Tollip. J Biol Chem. 2002; 277(9): 7059-65[DOI][PubMed]
  • 40. Fernandes AF, Zhou J, Zhang X, Bian Q, Sparrow J, Taylor A, et al. Oxidative inactivation of the proteasome in retinal pigment epithelial cells. A potential link between oxidative stress and up-regulation of interleukin-8. J Biol Chem. 2008; 283(30): 20745-53[DOI][PubMed]
  • 41. Pearson G, Robinson F, Beers Gibson T, Xu BE, Karandikar M, Berman K, et al. Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr Rev. 2001; 22(2): 153-83[DOI][PubMed]
  • 42. Riewe SD, Mans JJ, Hirano T, Katz J, Shiverick KT, Brown TA, et al. Human trophoblast responses to Porphyromonas gingivalis infection. Mol Oral Microbiol. 2010; 25(4): 252-9[PubMed]
  • 43. Qian Y, Takeuchi S, Dugu L, Tsuji G, Xie L, Nakahara T, et al. Hematopoietic progenitor kinase 1, mitogen-activated protein/extracellular signal-related protein kinase kinase kinase 1, and phosphomitogen-activated protein kinase kinase 4 are overexpressed in extramammary Paget disease. Am J Dermatopathol. 2011; 33(7): 681-6[DOI][PubMed]
  • 44. Roberts HC, Moseley R, Sloan AJ, Youde SJ, Waddington RJ. Lipopolysaccharide alters decorin and biglycan synthesis in rat alveolar bone osteoblasts: consequences for bone repair during periodontal disease. Eur J Oral Sci. 2008; 116(3): 207-16[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments