Jundishapur Journal of Microbiology

Published by: Kowsar

Association Between Toxin-Antitoxin Systems and Biofilm Formation

Sajedeh Karimi 1 , Sobhan Ghafourian 1 , Morovat Taheri Kalani 1 , Farid Azizi Jalilian 1 , Saeed Hemati 1 and Nourkhoda Sadeghifard 1 , *
Authors Information
1 Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR Iran
Article information
  • Jundishapur Journal of Microbiology: January 2015, 8 (1); e59746
  • Published Online: December 8, 2014
  • Article Type: Research Article
  • Received: September 1, 2013
  • Revised: April 3, 2014
  • Accepted: April 9, 2014
  • DOI: 10.5812/jjm.14540

To Cite: Karimi S, Ghafourian S, Taheri Kalani M, Azizi Jalilian F, Hemati S, et al. Association Between Toxin-Antitoxin Systems and Biofilm Formation, Jundishapur J Microbiol. 2015 ; 8(1):e59746. doi: 10.5812/jjm.14540.

Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Van Melderen L. Toxin-antitoxin systems: why so many, what for? Curr Opin Microbiol. 2010; 13(6): 781-5[DOI][PubMed]
  • 2. Kolodkin-Gal I, Engelberg-Kulka H. Induction of Escherichia coli chromosomal mazEF by stressful conditions causes an irreversible loss of viability. J Bacteriol. 2006; 188(9): 3420-3[DOI][PubMed]
  • 3. Erental A, Sharon I, Engelberg-Kulka H. Two programmed cell death systems in Escherichia coli: an apoptotic-like death is inhibited by the mazEF-mediated death pathway. PLoS Biol. 2012; 10(3)[DOI][PubMed]
  • 4. Engelberg-Kulka H, Sat B, Reches M, Amitai S, Hazan R. Bacterial programmed cell death systems as targets for antibiotics. Trends Microbiol. 2004; 12(2): 66-71[DOI][PubMed]
  • 5. Amitai S, Kolodkin-Gal I, Hananya-Meltabashi M, Sacher A, Engelberg-Kulka H. Escherichia coli MazF leads to the simultaneous selective synthesis of both "death proteins" and "survival proteins". PLoS Genet. 2009; 5(3)[DOI][PubMed]
  • 6. Schumacher MA, Piro KM, Xu W, Hansen S, Lewis K, Brennan RG. Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB. Science. 2009; 323(5912): 396-401[DOI][PubMed]
  • 7. Masuda Y, Ohtsubo E. Mapping and disruption of the chpB locus in Escherichia coli. J Bacteriol. 1994; 176(18): 5861-3[PubMed]
  • 8. Overgaard M, Borch J, Gerdes K. RelB and RelE of Escherichia coli form a tight complex that represses transcription via the ribbon-helix-helix motif in RelB. J Mol Biol. 2009; 394(2): 183-96[DOI][PubMed]
  • 9. Grady R, Hayes F. Axe-Txe, a broad-spectrum proteic toxin-antitoxin system specified by a multidrug-resistant, clinical isolate of Enterococcus faecium. Mol Microbiol. 2003; 47(5): 1419-32[PubMed]
  • 10. Christensen SK, Maenhaut-Michel G, Mine N, Gottesman S, Gerdes K, Van Melderen L. Overproduction of the Lon protease triggers inhibition of translation in Escherichia coli: involvement of the yefM-yoeB toxin-antitoxin system. Mol Microbiol. 2004; 51(6): 1705-17[PubMed]
  • 11. Wang X, Kim Y, Hong SH, Ma Q, Brown BL, Pu M, et al. Antitoxin MqsA helps mediate the bacterial general stress response. Nat Chem Biol. 2011; 7(6): 359-66[DOI][PubMed]
  • 12. Williams JJ, Hergenrother PJ. Exposing plasmids as the Achilles' heel of drug-resistant bacteria. Curr Opin Chem Biol. 2008; 12(4): 389-99[DOI][PubMed]
  • 13. Williams JJ, Halvorsen EM, Dwyer EM, DiFazio RM, Hergenrother PJ. Toxin-antitoxin (TA) systems are prevalent and transcribed in clinical isolates of Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus. FEMS Microbiol Lett. 2011; 322(1): 41-50[DOI][PubMed]
  • 14. Christensen SK, Mikkelsen M, Pedersen K, Gerdes K. RelE, a global inhibitor of translation, is activated during nutritional stress. Proc Natl Acad Sci U S A. 2001; 98(25): 14328-33[DOI][PubMed]
  • 15. Pecota DC, Wood TK. Exclusion of T4 phage by the hok/sok killer locus from plasmid R1. J Bacteriol. 1996; 178(7): 2044-50[PubMed]
  • 16. Magnuson RD. Hypothetical functions of toxin-antitoxin systems. J Bacteriol. 2007; 189(17): 6089-92[DOI][PubMed]
  • 17. Engelberg-Kulka H, Amitai S, Kolodkin-Gal I, Hazan R. Bacterial programmed cell death and multicellular behavior in bacteria. PLoS Genet. 2006; 2(10)[DOI][PubMed]
  • 18. Harrison JJ, Ceri H, Turner RJ. Multimetal resistance and tolerance in microbial biofilms. Nat Rev Microbiol. 2007; 5(12): 928-38[DOI][PubMed]
  • 19. Stewart PS. Mechanisms of antibiotic resistance in bacterial biofilms. Int J Med Microbiol. 2002; 292(2): 107-13[DOI][PubMed]
  • 20. Keren I, Shah D, Spoering A, Kaldalu N, Lewis K. Specialized persister cells and the mechanism of multidrug tolerance in Escherichia coli. J Bacteriol. 2004; 186(24): 8172-80[DOI][PubMed]
  • 21. Kim Y, Wood TK. Toxins Hha and CspD and small RNA regulator Hfq are involved in persister cell formation through MqsR in Escherichia coli. Biochem Biophys Res Commun. 2010; 391(1): 209-13[DOI][PubMed]
  • 22. Ren D, Bedzyk LA, Thomas SM, Ye RW, Wood TK. Gene expression in Escherichia coli biofilms. Appl Microbiol Biotechnol. 2004; 64(4): 515-24[DOI][PubMed]
  • 23. Queipo-Ortuno MI, De Dios Colmenero J, Macias M, Bravo MJ, Morata P. Preparation of bacterial DNA template by boiling and effect of immunoglobulin G as an inhibitor in real-time PCR for serum samples from patients with brucellosis. Clin Vaccine Immunol. 2008; 15(2): 293-6[DOI][PubMed]
  • 24. Subramanian P, Shanmugam N, Sivaraman U, Kumar S, Selvaraj S. Antiobiotic resistance pattern of biofilm-forming uropathogens isolated from catheterised patients in Pondicherry, India. Australas Med J. 2012; 5(7): 344-8[DOI][PubMed]
  • 25. O'Toole GA, Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol. 1998; 28(3): 449-61[PubMed]
  • 26. Vieira HL, Freire P, Arraiano CM. Effect of Escherichia coli morphogene bolA on biofilms. Appl Environ Microbiol. 2004; 70(9): 5682-4[DOI][PubMed]
  • 27. Stepanovic S, Cirkovic I, Ranin L, Svabic-Vlahovic M. Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface. Lett Appl Microbiol. 2004; 38(5): 428-32[DOI][PubMed]
  • 28. Lemos JA, Brown TA, Jr., Abranches J, Burne RA. Characteristics of Streptococcus mutans strains lacking the MazEF and RelBE toxin-antitoxin modules. FEMS Microbiol Lett. 2005; 253(2): 251-7[DOI][PubMed]
  • 29. Gonzalez Barrios AF, Zuo R, Hashimoto Y, Yang L, Bentley WE, Wood TK. Autoinducer 2 controls biofilm formation in Escherichia coli through a novel motility quorum-sensing regulator (MqsR, B3022). J Bacteriol. 2006; 188(1): 305-16[DOI][PubMed]
  • 30. Kasari V, Kurg K, Margus T, Tenson T, Kaldalu N. The Escherichia coli mqsR and ygiT genes encode a new toxin-antitoxin pair. J Bacteriol. 2010; 192(11): 2908-19[DOI][PubMed]
  • 31. Tsilibaris V, Maenhaut-Michel G, Mine N, Van Melderen L. What is the benefit to Escherichia coli of having multiple toxin-antitoxin systems in its genome? J Bacteriol. 2007; 189(17): 6101-8[DOI][PubMed]
  • 32. Kim Y, Wang X, Ma Q, Zhang XS, Wood TK. Toxin-antitoxin systems in Escherichia coli influence biofilm formation through YjgK (TabA) and fimbriae. J Bacteriol. 2009; 191(4): 1258-67[DOI][PubMed]
  • 33. Kolodkin-Gal I, Verdiger R, Shlosberg-Fedida A, Engelberg-Kulka H. A differential effect of E. coli toxin-antitoxin systems on cell death in liquid media and biofilm formation. PLoS One. 2009; 4(8)[DOI][PubMed]
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