Jundishapur Journal of Microbiology

Published by: Kowsar

Phenotypic and Genotypic Detection of Extended Spectrum β-lactamase and Carbapenemases Production Including bla TEM, bla PER and bla NDM-1 Genes Among Acinetobacter baumannii Clinical Isolates

Mojtaba Moosavian 1 , 2 , Mehrandokht Sirous 2 and Nasim Shams 2 , *
Authors Information
1 Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Article information
  • Jundishapur Journal of Microbiology: December 2017, 10 (12); e58206
  • Published Online: November 20, 2017
  • Article Type: Research Article
  • Received: July 28, 2017
  • Revised: September 2, 2017
  • Accepted: October 14, 2017
  • DOI: 10.5812/jjm.58206

To Cite: Moosavian M, Sirous M, Shams N. Phenotypic and Genotypic Detection of Extended Spectrum β-lactamase and Carbapenemases Production Including bla TEM, bla PER and bla NDM-1 Genes Among Acinetobacter baumannii Clinical Isolates, Jundishapur J Microbiol. 2017 ; 10(12):e58206. doi: 10.5812/jjm.58206.

Copyright © 2017, Jundishapur Journal of Microbiology. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
6. Conclusions
  • 1. Routray A, Lavanya P, Soniya R, Madhavan R. Multiplex PCR for genes encoding prevalent OXA and NDM-1 carbapenemases in Acinetobacter. J Pharmacy Res. 2013; 7(4): 324-6[DOI]
  • 2. Naas T, Bogaerts P, Bauraing C, Degheldre Y, Glupczynski Y, Nordmann P. Emergence of PER and VEB extended-spectrum beta-lactamases in Acinetobacter baumannii in Belgium. J Antimicrob Chemother. 2006; 58(1): 178-82[DOI][PubMed]
  • 3. Sechi LA, Karadenizli A, Deriu A, Zanetti S, Kolayli F, Balikci E, et al. PER-1 type beta-lactamase production in Acinetobacter baumannii is related to cell adhesion. Med Sci Monit. 2004; 10(6)-4[PubMed]
  • 4. Bush K, Jacoby GA. Updated functional classification of beta-lactamases. Antimicrob Agents Chemother. 2010; 54(3): 969-76[DOI][PubMed]
  • 5. Opazo A, Dominguez M, Bello H, Amyes SG, Gonzalez-Rocha G. OXA-type carbapenemases in Acinetobacter baumannii in South America. J Infect Dev Ctries. 2012; 6(4): 311-6[PubMed]
  • 6. European Centre for Disease Prevention and Control. . Carbapenem-resistant Acinetobacter baumannii in healthcare settings - 8 December 2016. 2016;
  • 7. Shamsizadeh Z, Nikaeen M, Nasr Esfahani B, Mirhoseini SH, Hatamzadeh M, Hassanzadeh A. Detection of antibiotic resistant Acinetobacter baumannii in various hospital environments: potential sources for transmission of Acinetobacter infections. Environ Health Prev Med. 2017; 22: 44[DOI]
  • 8. Mahon CR, Lehman DC, Manuselis G. Textbook of Diagnostic microbiology M. 2015; : 484-5
  • 9. Turton JF, Woodford N, Glover J, Yarde S, Kaufmann ME, Pitt TL. Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. J Clin Microbiol. 2006; 44(8): 2974-6[DOI][PubMed]
  • 10. CLSI. . Performance standard for antimicrobial susceptibility testing: Twenty-fourth informational supplement. ClSL document M100-S24. 2014;
  • 11. Shahcheraghi F, Nobari S, Rahmati Ghezelgeh F, Nasiri S, Owlia P, Nikbin VS, et al. First report of New Delhi metallo-beta-lactamase-1-producing Klebsiella pneumoniae in Iran. Microb Drug Resist. 2013; 19(1): 30-6[DOI][PubMed]
  • 12. Nordmann P, Poirel L, Carrer A, Toleman MA, Walsh TR. How To Detect NDM-1 Producers. J Clinical Microbiol. 2010; 49(2): 718-21[DOI]
  • 13. Naiemi NA, Duim B, Savelkoul PH, Spanjaard L, de Jonge E, Bart A, et al. Widespread transfer of resistance genes between bacterial species in an intensive care unit: implications for hospital epidemiology. J Clin Microbiol. 2005; 43(9): 4862-4[DOI][PubMed]
  • 14. Feizabadi MM, Fathollahzadeh B, Taherikalani M, Rasoolinejad M, Sadeghifard N, Aligholi M, et al. Antimicrobial susceptibility patterns and distribution of blaOXA genes among Acinetobacter spp. Isolated from patients at Tehran hospitals. Jpn J Infect Dis. 2008; 61(4): 274-8[PubMed]
  • 15. Goudarzi H, Douraghi M, Ghalavand Z, Goudarzi M. Assessment of antibiotic resistance pattern in Acinetobacter bumannii carrying bla OXA type genes isolated from hospitalized patients. Novel Biomedicine. 2013; 1(2): 54-61
  • 16. Shaykh Baygloo N, Bouzari M, Rahimi F, Abedini F, Yadegari S, Soroushnia M, et al. Identification of Genomic Species of Acinetobacter Isolated from Burns of ICU Patients. Arch Iran Med. 2015; 18(10): 638-42[PubMed]
  • 17. Zhao SY, Jiang DY, Xu PC, Zhang YK, Shi HF, Cao HL, et al. An investigation of drug-resistant Acinetobacter baumannii infections in a comprehensive hospital of East China. Ann Clin Microbiol Antimicrob. 2015; 14: 7[DOI][PubMed]
  • 18. Beceiro A, Fernandez-Cuenca F, Ribera A, Martinez-Martinez L, Pascual A, Vila J, et al. False extended-spectrum beta-lactamase detection in Acinetobacter spp. due to intrinsic susceptibility to clavulanic acid. J Antimicrob Chemother. 2008; 61(2): 301-8[DOI][PubMed]
  • 19. Kumar AV, Pillai VS, Dinesh KR, Karim S. The phenotypic detection of Carbapenemase in meropenem resistant Acinetobacter calcoaceticus baumannii complex in a tertiary care hospital in south India. J Clin Diagn Res. 2011; 5: 223-6
  • 20. John S, Balagurunathan R. Metallo beta lactamase producing Pseudomonas aeruginosa and Acinetobacter baumannii. Indian J Med Microbiol. 2011; 29(3): 302-4[DOI][PubMed]
  • 21. Heritier C, Poirel L, Nordmann P. Cephalosporinase over-expression resulting from insertion of ISAba1 in Acinetobacter baumannii. Clin Microbiol Infect. 2006; 12(2): 123-30[DOI][PubMed]
  • 22. Azimi L, Talebi M, Pourshafie MR, Owlia P, Rastegar Lari A. Characterization of Carbapenemases in Extensively Drug Resistance Acinetobacter baumannii in a Burn Care Center in Iran. Int J Mol Cell Med. 2015; 4(1): 46-53[PubMed]
  • 23. Rezaee MA, Pajand O, Nahaei MR, Mahdian R, Aghazadeh M, Ghojazadeh M, et al. Prevalence of Ambler class A beta-lactamases and ampC expression in cephalosporin-resistant isolates of Acinetobacter baumannii. Diagn Microbiol Infect Dis. 2013; 76(3): 330-4[DOI][PubMed]
  • 24. Bagheri Josheghani S, Moniri R, Firouzeh F, Sehat M, Dasteh Goli Y. Susceptibility pattern and distribution of oxacillinases andblaPER-1 genes among multidrug resistant Acinetobacter baumannii in a teaching hospital in Iran. J Pathog. 2015; 2015: 7[DOI]
  • 25. Alan TF, Goudarzi H, Fallah F, Hashemi A, Doustdar F, Bostan H. Detection of blaNDM, blaDIM, blaIMP, blaVIM and blaCTX-M-15 beta-lactamase genes among pseudomonas aeruginosa and acinetobacter baumannii strains isolated from two hospitals of Tehran, Iran. Novel Biomed. 2016; 4(4): 153-8
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments