Jundishapur Journal of Microbiology

Published by: Kowsar

Pseudomonas aeruginosa as a Powerful Biofilm Producer and Positive Action of Amikacin Against Isolates From Chronic Wounds

Kashif Rahim 1 , * , Shamim Saleha 2 , Abdul Basit 3 , Xudong Zhu 1 , Iqbal Ahmed 4 , Liang Huo 1 , Ping Zhang 1 , Bakhtawar Usman 4 , Shahzad Munir 5 and Octavio Luiz Franco 6 , 7
Authors Information
1 Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing 100875, China
2 Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology (KUST), Khyber Pakhtunkhwa Kohat, 26000, Pakistan
3 College of Biological Sciences, China Agricultural University, Beijing 100193, China
4 Harbin Veterinary Research Institute, CAAS-Michigan State University Joint Laboratory of Innate Immunity, State Key Lab of VET and Biotech, Chinese Academy of Agricultural Sciences, Harbin, China
5 Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan, China
6 Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brazil 70790-160, Brazil
7 S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande CEP 79.117-900, Brazil
Article information
  • Jundishapur Journal of Microbiology: October 2017, 10 (10); e57564
  • Published Online: October 28, 2017
  • Article Type: Research Article
  • Received: July 4, 2017
  • Revised: July 27, 2017
  • Accepted: August 26, 2017
  • DOI: 10.5812/jjm.57564

To Cite: Rahim K, Saleha S, Basit A, Zhu X, Ahmed I, et al. Pseudomonas aeruginosa as a Powerful Biofilm Producer and Positive Action of Amikacin Against Isolates From Chronic Wounds, Jundishapur J Microbiol. 2017 ; 10(10):e57564. doi: 10.5812/jjm.57564.

Abstract
Copyright © 2017, Jundishapur Journal of Microbiology. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
6. Conclusions
Acknowledgements
Footnotes
References
  • 1. Gjodsbol K, Christensen JJ, Karlsmark T, Jorgensen B, Klein BM, Krogfelt KA. Multiple bacterial species reside in chronic wounds: a longitudinal study. Int Wound J. 2006; 3(3): 225-31[DOI][PubMed]
  • 2. Davis R, Brown PD. Multiple antibiotic resistance index, fitness and virulence potential in respiratory Pseudomonas aeruginosa from Jamaica. J Med Microbiol. 2016; [DOI][PubMed]
  • 3. Mantero M, Gramegna A, Pizzamiglio G, D'Adda A, Tarsia P, Blasi F. Once daily aerosolised tobramycin in adult patients with cystic fibrosis in the management of Pseudomonas aeruginosa chronic infection. Multidiscip Respir Med. 2017; 12: 2[DOI][PubMed]
  • 4. Hoiby N, Bjarnsholt T, Givskov M, Molin S, Ciofu O. Antibiotic resistance of bacterial biofilms. Int J Antimicrob Agents. 2010; 35(4): 322-32[DOI][PubMed]
  • 5. Rahim K, Saleha S, Zhu X, Huo L, Basit A, Franco OL. Bacterial Contribution in Chronicity of Wounds. Microb Ecol. 2017; 73(3): 710-21[DOI][PubMed]
  • 6. Dowd SE, Sun Y, Secor PR, Rhoads DD, Wolcott BM, James GA, et al. Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing. BMC Microbiol. 2008; 8: 43[DOI][PubMed]
  • 7. Oates JL. Genome sequencing of two chronic wound isolated pseudomonas aeruginosa strains to understand adaptation and antibiotic resistance. 2015;
  • 8. Whitchurch CB, Tolker-Nielsen T, Ragas PC, Mattick JS. Extracellular DNA required for bacterial biofilm formation. Science. 2002; 295(5559): 1487[DOI][PubMed]
  • 9. Harmsen M, Yang L, Pamp SJ, Tolker-Nielsen T. An update on Pseudomonas aeruginosa biofilm formation, tolerance, and dispersal. FEMS Immunol Med Microbiol. 2010; 59(3): 253-68[DOI][PubMed]
  • 10. Nithya C, Begum MF, Pandian SK. Marine bacterial isolates inhibit biofilm formation and disrupt mature biofilms of Pseudomonas aeruginosa PAO1. Appl Microbiol Biotechnol. 2010; 88(1): 341-58[DOI][PubMed]
  • 11. Malone M, Swanson T. Biofilm-based wound care: the importance of debridement in biofilm treatment strategies. Br J Community Nurs. 2017; 22-5[DOI][PubMed]
  • 12. James GA, Swogger E, Wolcott R, Pulcini E, Secor P, Sestrich J, et al. Biofilms in chronic wounds. Wound Repair Regen. 2008; 16(1): 37-44[DOI][PubMed]
  • 13. Malone M, Bjarnsholt T, McBain AJ, James GA, Stoodley P, Leaper D, et al. The prevalence of biofilms in chronic wounds: a systematic review and meta-analysis of published data. J Wound Care. 2017; 26(1): 20-5[DOI][PubMed]
  • 14. Gaynes R, Edwards JR, National Nosocomial Infections Surveillance S. Overview of nosocomial infections caused by gram-negative bacilli. Clin Infect Dis. 2005; 41(6): 848-54[DOI][PubMed]
  • 15. Aloush V, Navon-Venezia S, Seigman-Igra Y, Cabili S, Carmeli Y. Multidrug-resistant Pseudomonas aeruginosa: risk factors and clinical impact. Antimicrob Agents Chemother. 2006; 50(1): 43-8[DOI][PubMed]
  • 16. Rayber GE. The diabetic foot. 2001; : 13-32
  • 17. Flamm RK, Weaver MK, Thornsberry C, Jones ME, Karlowsky JA, Sahm DF. Factors associated with relative rates of antibiotic resistance in Pseudomonas aeruginosa isolates tested in clinical laboratories in the United States from 1999 to 2002. Antimicrob Agents Chemother. 2004; 48(7): 2431-6[DOI][PubMed]
  • 18. Obritsch MD, Fish DN, MacLaren R, Jung R. National surveillance of antimicrobial resistance in Pseudomonas aeruginosa isolates obtained from intensive care unit patients from 1993 to 2002. Antimicrob Agents Chemother. 2004; 48(12): 4606-10[DOI][PubMed]
  • 19. Dortet L, Poirel L, Nordmann P. Rapid identification of carbapenemase types in Enterobacteriaceae and Pseudomonas spp. by using a biochemical test. Antimicrob Agents Chemother. 2012; 56(12): 6437-40[DOI][PubMed]
  • 20. Sneath PH, Mair NS, Sharpe ME. JG Holt Bergey's Manual of Systematic Bacteriology, 2. 1986;
  • 21. Freeman DJ, Falkiner FR, Keane CT. New method for detecting slime production by coagulase negative staphylococci. J Clin Pathol. 1989; 42(8): 872-4[PubMed]
  • 22. da Silva Filho LV, Levi JE, Oda Bento CN, da Silva Ramos SR, Rozov T. PCR identification of Pseudomonas aeruginosa and direct detection in clinical samples from cystic fibrosis patients. J Med Microbiol. 1999; 48(4): 357-61[DOI][PubMed]
  • 23. Ammons MC, Ward LS, Fisher ST, Wolcott RD, James GA. In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol. Int J Antimicrob Agents. 2009; 33(3): 230-6[DOI][PubMed]
  • 24. Bjarnsholt T, Kirketerp-Moller K, Jensen PO, Madsen KG, Phipps R, Krogfelt K, et al. Why chronic wounds will not heal: a novel hypothesis. Wound Repair Regen. 2008; 16(1): 2-10[DOI][PubMed]
  • 25. Kirketerp-Moller K, Jensen PO, Fazli M, Madsen KG, Pedersen J, Moser C, et al. Distribution, organization, and ecology of bacteria in chronic wounds. J Clin Microbiol. 2008; 46(8): 2717-22[DOI][PubMed]
  • 26. Thomsen TR, Aasholm MS, Rudkjobing VB, Saunders AM, Bjarnsholt T, Givskov M, et al. The bacteriology of chronic venous leg ulcer examined by culture-independent molecular methods. Wound Repair Regen. 2010; 18(1): 38-49[DOI][PubMed]
  • 27. Madigan MT, Martinko JM, Parker J. Brock biology of microorganisms. 1997; 11
  • 28. Watters C, DeLeon K, Trivedi U, Griswold JA, Lyte M, Hampel KJ, et al. Pseudomonas aeruginosa biofilms perturb wound resolution and antibiotic tolerance in diabetic mice. Med Microbiol Immunol. 2013; 202(2): 131-41[DOI][PubMed]
  • 29. Driscoll JA, Brody SL, Kollef MH. The epidemiology, pathogenesis and treatment of Pseudomonas aeruginosa infections. Drugs. 2007; 67(3): 351-68[PubMed]
  • 30. Azzopardi EA, Azzopardi E, Camilleri L, Villapalos J, Boyce DE, Dziewulski P, et al. Gram negative wound infection in hospitalised adult burn patients--systematic review and metanalysis. PLoS One. 2014; 9(4)[DOI][PubMed]
  • 31. Storm-Versloot MN, Vos CG, Ubbink DT, Vermeulen H. Topical silver for preventing wound infection. Cochrane Database Syst Rev. 2010; (3)[DOI][PubMed]
  • 32. Sanchez CJ, Mende K, Beckius ML, Akers KS, Romano DR, Wenke JC, et al. Biofilm formation by clinical isolates and the implications in chronic infections. BMC Infect Dis. 2013; 13: 47[DOI][PubMed]
  • 33. Dowd SE, Wolcott RD, Sun Y, McKeehan T, Smith E, Rhoads D. Polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP). PLoS One. 2008; 3(10)[DOI][PubMed]
  • 34. Baffoni M, Bessa LJ, Grande R, Di Giulio M, Mongelli M, Ciarelli A, et al. Laser irradiation effect on Staphylococcus aureus and Pseudomonas aeruginosa biofilms isolated from venous leg ulcer. Int Wound J. 2012; 9(5): 517-24[DOI][PubMed]
  • 35. Moore NM, Flaws ML. Antimicrobial resistance mechanisms in Pseudomonas aeruginosa. Clin Lab Sci. 2011; 24(1): 47-51[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments