Jundishapur Journal of Microbiology

Published by: Kowsar

Survey of CTX-M Gene Frequency in Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Isolates Using the Combination Disk and PCR Methods in Ahvaz, Iran

Mojtaba Moosavian 1 , 2 and Nazanin Ahmadkhosravy 2 , *
Authors Information
1 Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
2 Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jundishapur Journal of Microbiology: November 01, 2016, 9 (11); e40423
  • Published Online: September 26, 2016
  • Article Type: Research Article
  • Received: July 5, 2016
  • Accepted: September 19, 2016
  • DOI: 10.5812/jjm.40423

To Cite: Moosavian M, Ahmadkhosravy N. Survey of CTX-M Gene Frequency in Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Isolates Using the Combination Disk and PCR Methods in Ahvaz, Iran, Jundishapur J Microbiol. 2016 ; 9(11):e40423. doi: 10.5812/jjm.40423.

Abstract
Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Introduction
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
References
  • 1. Ambler RP. The structure of beta-lactamases. Philos Trans R Soc Lond B Biol Sci. 1980; 289(1036): 321-31[PubMed]
  • 2. Medeiros A, Mayer K, Opal SM. Plasmid Beta-lactamases. AntimicrobiolNewsl. 1988; 5: 61-5
  • 3. Bush K. Classification of beta-lactamases: groups 1, 2a, 2b, and 2b'. Antimicrob Agents Chemother. 1989; 33(3): 264-70[PubMed]
  • 4. Al-Agamy MH, Shibl AM, Tawfik AF. Prevalence and molecular characterization of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in Riyadh, Saudi Arabia. Ann Saudi Med. 2009; 29(4): 253-7[PubMed]
  • 5. Mohamudha PR, Srinivas AN, Rahul D, Harish BN, Parija SC. Molecular epidemiology of Multidrug resistant Extended-Spectrum β-Lactamase Producing Klebsiella pneumoniae outbreak in a neonatal intensive care unit. IJCRIMPH. 2010; 2(7): 226-38
  • 6. Munier GK, Johnson CL, Snyder JW, Moland ES, Hanson ND, Thomson KS. Positive extended-spectrum-beta-lactamase (ESBL) screening results may be due to AmpC beta-lactamases more often than to ESBLs. J Clin Microbiol. 2010; 48(2): 673-4[DOI][PubMed]
  • 7. Bonnet R. Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother. 2004; 48(1): 1-14[PubMed]
  • 8. Bradford PA. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev. 2001; 14(4): 933-51[DOI][PubMed]
  • 9. Monstein HJ, Tarnberg M, Nilsson LE. Molecular identification of CTX-M and blaOXY/K1 beta-lactamase genes in Enterobacteriaceae by sequencing of universal M13-sequence tagged PCR-amplicons. BMC Infect Dis. 2009; 9: 7[DOI][PubMed]
  • 10. Edelstein M, Pimkin M, Palagin I, Edelstein I, Stratchounski L. Prevalence and molecular epidemiology of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Russian hospitals. Antimicrob Agents Chemother. 2003; 47(12): 3724-32[PubMed]
  • 11. Govinden U, Mocktar C, Moodley P, Stum AW, Essack SY. Geographical evolution of the CTX-M-lactamase an update. African J Biotech. 2007; 6(7): 831-9
  • 12. Valenzuela de Silva EM, Mantilla Anaya JR, Reguero Reza MT, Gonzalez Mejia EB, Pulido Manrique IY, Dario Llerena I, et al. Detection of CTX-M-1, CTX-M-15, and CTX-M-2 in clinical isolates of Enterobacteriaceae in Bogota, Colombia. J Clin Microbiol. 2006; 44(5): 1919-20[DOI][PubMed]
  • 13. Performance standards for antimicrobialsusebtibility testing:20th information supplement , document M100-S20. 2010;
  • 14. Sambrook MJ, Russel DW. Molecular colning Laboratory manual . 2001; 2
  • 15. Amaral SN, Peixe LV, Machado E. Characterization of ctx-m-type extended-spectrum beta-lactamases (esbls) among enterobacteriaceae from a portuguese hospital. 2009; 6: 254-63
  • 16. Mirzaee M, Pourmand MR, Chitsaz M, Mansouri S. Antibiotic resistance to third generation cephalosporins due to CTX-M-Type extended-spectrum β-lactamases in clinical isolates of Escherichia coli. I J Public Health. 2009; 38(1): 10-7
  • 17. Rossolini GM, D'Andrea MM, Mugnaioli C. The spread of CTX-M-type extended-spectrum beta-lactamases. Clin Microbiol Infect. 2008; 14 Suppl 1: 33-41[DOI][PubMed]
  • 18. Livermore DM. beta-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev. 1995; 8(4): 557-84[PubMed]
  • 19. Canton R, Novais A, Valverde A, Machado E, Peixe L, Baquero F, et al. Prevalence and spread of extended-spectrum beta-lactamase-producing Enterobacteriaceae in Europe. Clin Microbiol Infect. 2008; 14 Suppl 1: 144-53[DOI][PubMed]
  • 20. Philippon A, Arlet G, Lagrange PH. Origin and impact of plasmid-mediated extended-spectrum beta-lactamases. Eur J Clin Microbiol Infect Dis. 1994; 13 Suppl 1-29[PubMed]
  • 21. Luzzaro F, Mezzatesta M, Mugnaioli C, Perilli M, Stefani S, Amicosante G, et al. Trends in production of extended-spectrum beta-lactamases among enterobacteria of medical interest: report of the second Italian nationwide survey. J Clin Microbiol. 2006; 44(5): 1659-64[DOI][PubMed]
  • 22. Riaz S, Faisal M, Hasnain S. Prevalence and comparison of Beta-lactamase producing Escherichia coli and Klebsiella spp from clinical and environmental sources in Lahore, Pakistan. African J Microbiol Res. 2012; 6(2): 465-70
  • 23. Ramazanzadeh R. Etiologic agents and extended-spectrum beta-lactamase production in urinary tract infections in Sanandaj, Iran. Eastern J Med. 2010; 15(2): 57-62
  • 24. Moosavian M, Deiham B. Distribution of TEM, SHV and CTX-M Genes among ESBL-producing Enterobacteriaceae isolates in Iran. African J Microbiol Res. 2012; 6(26): 5433-9
  • 25. Nasehi L, Shahcheraghi F, Nikbin V, Nematzadeh S. PER, CTX-M, TEM and SHV Beta-lactamases in clinical isolates of Klebsiella pneumoniae isolated from Tehran, Iran. Iranian J Basic Med Sci. 2010; 13(3): 111-8
  • 26. Maina D, Revathi G, Kariuki S, Ozwara H. Genotypes and cephalosporin susceptibility in extended-spectrum beta-lactamase producing enterobacteriaceae in the community. J Infect Dev Ctries. 2012; 6(6): 470-7[PubMed]
  • 27. Peirano G, Pitout JD. Molecular epidemiology of Escherichia coli producing CTX-M beta-lactamases: the worldwide emergence of clone ST131 O25:H4. Int J Antimicrob Agents. 2010; 35(4): 316-21[DOI][PubMed]
  • 28. Soltan Dallal MM, Shirazi MH. The prevalence of extended-spectrum beta-lactamases and CTX-M-1 producing Escherichia coli in urine samples collected at Tabriz city Hospitals. Tehran Univ Med J TUMS Publ. 2011; 69(5): 273-8
  • 29. Shahcheraghi F, Nasiri S, Noveiri H. The Survey of Genes Encoding Beta-Lactamases, in Escherichia Coli Resistant to Beta-Lactam and Non-Beta-Lactam Antibiotics. Iran J Basic Med Sci. 2010; 13(1): 230-7
  • 30. Bertrand S, Weill FX, Cloeckaert A, Vrints M, Mairiaux E, Praud K, et al. Clonal emergence of extended-spectrum beta-lactamase (CTX-M-2)-producing Salmonella enterica serovar Virchow isolates with reduced susceptibilities to ciprofloxacin among poultry and humans in Belgium and France (2000 to 2003). J Clin Microbiol. 2006; 44(8): 2897-903[DOI][PubMed]
  • 31. Poirel L, Decousser JW, Nordmann P. Insertion sequence ISEcp1B is involved in expression and mobilization of a bla(CTX-M) beta-lactamase gene. Antimicrob Agents Chemother. 2003; 47(9): 2938-45[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments