Jundishapur Journal of Microbiology

Published by: Kowsar

Evaluation of an Assay Based On Multiple Detection Temperature Technique for Simultaneous Detection of Viral Gastroenteritis-Causing Pathogens

Jeong-Hyun Han 1 , Sun-Hyung Kim 2 and Soon-Young Paik 1 , *
Authors Information
1 Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
2 Seegene Institute of Life Sciences, Seoul, Republic of Korea
Article information
  • Jundishapur Journal of Microbiology: March 01, 2017, 10 (3); e43548
  • Published Online: January 31, 2017
  • Article Type: Research Article
  • Received: November 2, 2016
  • Revised: January 19, 2017
  • Accepted: January 24, 2017
  • DOI: 10.5812/jjm.43548

To Cite: Han J, Kim S, Paik S. Evaluation of an Assay Based On Multiple Detection Temperature Technique for Simultaneous Detection of Viral Gastroenteritis-Causing Pathogens, Jundishapur J Microbiol. 2017 ; 10(3):e43548. doi: 10.5812/jjm.43548.

Copyright © 2017, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Liesenfeld O, Lehman L, Hunfeld KP, Kost G. Molecular diagnosis of sepsis: New aspects and recent developments. Eur J Microbiol Immunol (Bp). 2014; 4(1): 1-25[DOI][PubMed]
  • 2. V Ramana K. Molecular Diagnostic Methods and Their Application to Patient Care: Clinical Microbiologist’s Perspective. Am J Clin Med Res. 2014; 2(1): 8-13[DOI]
  • 3. Buss SN, Leber A, Chapin K, Fey PD, Bankowski MJ, Jones MK, et al. Multicenter evaluation of the BioFire FilmArray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. J Clin Microbiol. 2015; 53(3): 915-25[DOI][PubMed]
  • 4. Cho CH, Chulten B, Lee CK, Nam MH, Yoon SY, Lim CS, et al. Evaluation of a novel real-time RT-PCR using TOCE technology compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses. J Clin Virol. 2013; 57(4): 338-42[DOI][PubMed]
  • 5. Kim DH, Lee JH, Ha JS, Ryoo NH, Jeon DS, Kim JR. [Evaluation of the usefulness of selective chromogenic agar medium (chromID VRE) and multiplex PCR method for the detection of vancomycin-resistant enterococci]. Korean J Lab Med. 2010; 30(6): 631-6[DOI][PubMed]
  • 6. Lehours P, Siffre E, Megraud F. DPO multiplex PCR as an alternative to culture and susceptibility testing to detect Helicobacter pylori and its resistance to clarithromycin. BMC Gastroenterol. 2011; 11: 112[DOI][PubMed]
  • 7. Navarro E, Serrano-Heras G, Castano MJ, Solera J. Real-time PCR detection chemistry. Clin Chim Acta. 2015; 439: 231-50[DOI][PubMed]
  • 8. Lee DH. TOCE: innovative technology for high multiplex real-time PCR. Seegene Bull. 2012; 1: 5-10
  • 9. Lee YJ, Kim D, Lee K, Chun JY. Single-channel multiplexing without melting curve analysis in real-time PCR. Sci Rep. 2014; 4: 7439[DOI][PubMed]
  • 10. Storch GA. Diagnostic virology. Clin Infect Dis. 2000; 31(3): 739-51[DOI][PubMed]
  • 11. Hou FQ, Wang Y, Li J, Wang GQ, Liu Y. Management of acute diarrhea in adults in China: a cross-sectional survey. BMC Public Health. 2013; 13: 41[DOI][PubMed]
  • 12. Manatsathit S, Dupont HL, Farthing M, Kositchaiwat C, Leelakusolvong S, Ramakrishna BS, et al. Guideline for the management of acute diarrhea in adults. J Gastroenterol Hepatol. 2002; 17 Suppl-71[PubMed]
  • 13. Elliott EJ. Acute gastroenteritis in children. BMJ. 2007; 334(7583): 35-40[DOI][PubMed]
  • 14. Clark B, McKendrick M. A review of viral gastroenteritis. Curr Opin Infect Dis. 2004; 17(5): 461-9[PubMed]
  • 15. Dung TT, Phat VV, Nga TV, My PV, Duy PT, Campbell JI, et al. The validation and utility of a quantitative one-step multiplex RT real-time PCR targeting rotavirus A and norovirus. J Virol Methods. 2013; 187(1): 138-43[DOI][PubMed]
  • 16. Gautam R, Mijatovic-Rustempasic S, Esona MD, Tam KI, Quaye O, Bowen MD. One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix(R) and RotaTeq(R)) in stool samples. PeerJ. 2016; 4[DOI][PubMed]
  • 17. Jiang Y, Fang L, Shi X, Zhang H, Li Y, Lin Y, et al. Simultaneous detection of five enteric viruses associated with gastroenteritis by use of a PCR assay: a single real-time multiplex reaction and its clinical application. J Clin Microbiol. 2014; 52(4): 1266-8[DOI][PubMed]
  • 18. Pang XL, Preiksaitis JK, Lee B. Multiplex real time RT-PCR for the detection and quantitation of norovirus genogroups I and II in patients with acute gastroenteritis. J Clin Virol. 2005; 33(2): 168-71[DOI][PubMed]
  • 19. Svraka S, van der Veer B, Duizer E, Dekkers J, Koopmans M, Vennema H. Novel approach for detection of enteric viruses to enable syndrome surveillance of acute viral gastroenteritis. J Clin Microbiol. 2009; 47(6): 1674-9[DOI][PubMed]
  • 20. van Maarseveen NM, Wessels E, de Brouwer CS, Vossen AC, Claas EC. Diagnosis of viral gastroenteritis by simultaneous detection of Adenovirus group F, Astrovirus, Rotavirus group A, Norovirus genogroups I and II, and Sapovirus in two internally controlled multiplex real-time PCR assays. J Clin Virol. 2010; 49(3): 205-10[DOI][PubMed]
  • 21. Higgins RR, Beniprashad M, Cardona M, Masney S, Low DE, Gubbay JB. Evaluation and verification of the Seeplex Diarrhea-V ACE assay for simultaneous detection of adenovirus, rotavirus, and norovirus genogroups I and II in clinical stool specimens. J Clin Microbiol. 2011; 49(9): 3154-62[DOI][PubMed]
  • 22. Hwang B. M. , Lee D. Y. , Chung G. T. , Yoo CK. Laboratory surveillance of viral acute gastroenteritis in Korea, 2014. Public Health Week Rep PHWR. 2015; 8(49): 1172-7
  • 23. Liu J, Kibiki G, Maro V, Maro A, Kumburu H, Swai N, et al. Multiplex reverse transcription PCR Luminex assay for detection and quantitation of viral agents of gastroenteritis. J Clin Virol. 2011; 50(4): 308-13[DOI][PubMed]
  • 24. Corcoran MS, van Well GT, van Loo IH. Diagnosis of viral gastroenteritis in children: interpretation of real-time PCR results and relation to clinical symptoms. Eur J Clin Microbiol Infect Dis. 2014; 33(10): 1663-73[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments