Jundishapur Journal of Microbiology

Published by: Kowsar

Optimization of Recombinant Expression of Synthetic Bacterial Phytase in Pichia pastoris Using Response Surface Methodology

Ali Akbarzadeh 1 , Ehsan Dehnavi 2 , Mojtaba Aghaeepoor 2 , 3 and Jafar Amani 1 , *
Authors Information
1 Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
2 Gene Transfer Pioneers Research Group, Shahid Beheshti University, Tehran, IR Iran
3 Semnan Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, IR Iran
Article information
  • Jundishapur Journal of Microbiology: December 01, 2015, 8 (12); e27553
  • Published Online: December 26, 2015
  • Article Type: Research Article
  • Received: February 25, 2015
  • Revised: June 19, 2015
  • Accepted: July 13, 2015
  • DOI: 10.5812/jjm.27553

To Cite: Akbarzadeh A, Dehnavi E, Aghaeepoor M, Amani J. Optimization of Recombinant Expression of Synthetic Bacterial Phytase in Pichia pastoris Using Response Surface Methodology, Jundishapur J Microbiol. 2015 ; 8(12):e27553. doi: 10.5812/jjm.27553.

Abstract
Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Chen CC, Wu PH, Huang CT, Cheng KJ. A Pichia pastoris fermentation strategy for enhancing the heterologous expression of an Escherichia coli phytase. Enzyme Microb Technol. 2004; 35(4): 315-20[DOI]
  • 2. Xiong AS, Yao QH, Peng RH, Han PL, Cheng ZM, Li Y. High level expression of a recombinant acid phytase gene in Pichia pastoris. J Appl Microbiol. 2005; 98(2): 418-28[DOI][PubMed]
  • 3. Rao DE, Rao KV, Reddy VD. Cloning and expression of Bacillus phytase gene (phy) in Escherichia coli and recovery of active enzyme from the inclusion bodies. J Appl Microbiol. 2008; 105(4): 1128-37[DOI][PubMed]
  • 4. Zinin NV, Serkina AV, Gelfand MS, Shevelev AB, Sineoky SP. Gene cloning, expression and characterization of novel phytase from Obesumbacterium proteus. FEMS Microbiol Lett. 2004; 236(2): 283-90[DOI][PubMed]
  • 5. Stahl CH, Wilson DB, Lei XG. Comparison of extracellular Escherichia coli AppA phytases expressed in Streptomyces lividans and Pichia pastoris. Biotechnol Lett. 2003; 25(10): 827-31[PubMed]
  • 6. Guerrero-Olazaran M, Rodriguez-Blanco L, Carreon-Trevino JG, Gallegos-Lopez JA, Viader-Salvado JM. Expression of a Bacillus phytase C gene in Pichia pastoris and properties of the recombinant enzyme. Appl Environ Microbiol. 2010; 76(16): 5601-8[DOI][PubMed]
  • 7. Ushasree MV, Vidya J, Pandey A. Gene cloning and soluble expression of Aspergillus niger phytase in E. coli cytosol via chaperone co-expression. Biotechnol Lett. 2014; 36(1): 85-91[DOI][PubMed]
  • 8. Xuan NT, Hang MT, Thanh VN. Cloning and over Expression of an Aspergillus niger XP Phytase Gene (phyA) in Pichia pastoris. World Acad Sci Engin Technol. 2009; 56: 750-3
  • 9. Johnson SC, Yang M, Murthy PP. Heterologous expression and functional characterization of a plant alkaline phytase in Pichia pastoris. Protein Expr Purif. 2010; 74(2): 196-203[DOI][PubMed]
  • 10. Tai HM, Yin LJ, Chen WC, Jiang ST. Overexpression of Escherichia coli phytase in Pichia pastoris and its biochemical properties. J Agric Food Chem. 2013; 61(25): 6007-15[DOI][PubMed]
  • 11. Lassen SF, Breinholt J, Ostergaard PR, Brugger R, Bischoff A, Wyss M, et al. Expression, gene cloning, and characterization of five novel phytases from four basidiomycete fungi: Peniophora lycii, Agrocybe pediades, a Ceriporia sp., and Trametes pubescens. Appl Environ Microbiol. 2001; 67(10): 4701-7[PubMed]
  • 12. Wyss M, Brugger R, Kronenberger A, Remy R, Fimbel R, Oesterhelt G, et al. Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties. Appl Environ Microbiol. 1999; 65(2): 367-73[PubMed]
  • 13. Rodriguez E, Wood ZA, Karplus PA, Lei XG. Site-directed mutagenesis improves catalytic efficiency and thermostability of Escherichia coli pH 2.5 acid phosphatase/phytase expressed in Pichia pastoris. Arch Biochem Biophys. 2000; 382(1): 105-12[DOI][PubMed]
  • 14. Akbarzadeh A, Ranaei Siadat SO, Zamani MR, Motallebi M, Barshan Tashnizi M. Comparison of biochemical properties of recombinant endoglucanase II of Trichoderma reesei in methylotrophic yeasts, Pichia pastoris and Hansenula polymorpha. Progress Biol Sci. 2013; 3(1): 108-17
  • 15. Gellissen G, Kunze G, Gaillardin C, Cregg JM, Berardi E, Veenhuis M, et al. New yeast expression platforms based on methylotrophic Hansenula polymorpha and Pichia pastoris and on dimorphic Arxula adeninivorans and Yarrowia lipolytica - a comparison. FEMS Yeast Res. 2005; 5(11): 1079-96[DOI][PubMed]
  • 16. Cregg JM, Cereghino JL, Shi J, Higgins DR. Recombinant protein expression in Pichia pastoris. Mol Biotechnol. 2000; 16(1): 23-52
  • 17. Francis DM, Page R. Strategies to optimize protein expression in E. coli. Curr Protoc Protein Sci. 2010; -29[DOI][PubMed]
  • 18. Chambers SP, Swalley SE. Designing experiments for high-throughput protein expression. Methods Mol Biol. 2009; 498: 19-29[DOI][PubMed]
  • 19. Maharjan S, Singh B, Bok JD, Kim JI, Jiang T, Cho CS, et al. Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli. PLoS One. 2014; 9(5)[DOI][PubMed]
  • 20. Muntari B, Amid A, Mel M, Jami MS, Salleh HM. Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology. AMB Express. 2012; 2: 12[DOI][PubMed]
  • 21. Golovan S, Wang G, Zhang J, Forsberg CW. Characterization and overproduction of the Escherichia coli appA encoded bifunctional enzyme that exhibits both phytase and acid phosphatase activities. Can J Microbiol. 2000; 46(1): 59-71[PubMed]
  • 22. Bai J, Swartz DJ, Protasevich ,I, Brouillette CG, Harrell PM, Hildebrandt E, et al. A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris. PLoS One. 2011; 6(8)[DOI][PubMed]
  • 23. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72: 248-54[PubMed]
  • 24. Li X, Xu T, Ma X, Guo K, Kai L, Zhao Y, et al. Optimization of culture conditions for production of cis-epoxysuccinic acid hydrolase using response surface methodology. Bioresour Technol. 2008; 99(13): 5391-6[DOI][PubMed]
  • 25. Bae HD, Yanke LJ, Cheng KJ, Selinger LB. A novel staining method for detecting phytase activity. J Microbiol Methods. 1999; 39(1): 17-22[PubMed]
  • 26. Salihu A, Alam MZ, AbdulKarim MI, Salleh HM. Optimization of lipase production by Candida cylindracea in palm oil mill effluent based medium using statistical experimental design. J Mol Catalysis Enzym. 2011; 69(1): 66-73
  • 27. Akbarzadeh A, Ranaei Siadat SO, Motallebi M, Zamani MR, Barshan Tashnizi M, Moshtaghi S. Characterization and high level expression of acidic endoglucanase in Pichia pastoris. Appl Biochem Biotechnol. 2014; 172(4): 2253-65[DOI][PubMed]
  • 28. Wang H, Wang Q, Zhang F, Huang Y, Ji Y, Hou Y. Protein expression and purification of human Zbtb7A in Pichia pastoris via gene codon optimization and synthesis. Protein Expr Purif. 2008; 60(2): 97-102[DOI][PubMed]
  • 29. Sinclair G, Choy FY. Synonymous codon usage bias and the expression of human glucocerebrosidase in the methylotrophic yeast, Pichia pastoris. Protein Expr Purif. 2002; 26(1): 96-105[PubMed]
  • 30. Huang H, Yang P, Luo H, Tang H, Shao N, Yuan T, et al. High-level expression of a truncated 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentation. Appl Microbiol Biotechnol. 2008; 78(1): 95-103[DOI][PubMed]
  • 31. Batra J, Beri D, Mishra S. Response surface methodology based optimization of beta-glucosidase production from Pichia pastoris. Appl Biochem Biotechnol. 2014; 172(1): 380-93[DOI][PubMed]
  • 32. Yu Y, Zhou X, Wu S, Wei T, Yu L. High-yield production of the human lysozyme by Pichia pastoris SMD1168 using response surface methodology and high-cell-density fermentation. Electron J Biotechnol. 2014; 17(6): 311-6[DOI]
  • 33. Cao W, Li H, Zhang J, Li D, Acheampong DO, Chen Z, et al. Periplasmic expression optimization of VEGFR2 D3 adopting response surface methodology: antiangiogenic activity study. Protein Expr Purif. 2013; 90(2): 55-66[DOI][PubMed]
  • 34. Hong F, Meinander NQ, Jonsson LJ. Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris. Biotechnol Bioeng. 2002; 79(4): 438-49[DOI][PubMed]
  • 35. Greiner R, Konietzny U, Jany KD. Purification and characterization of two phytases from Escherichia coli. Arch Biochem Biophys. 1993; 303(1): 107-13[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments