Jundishapur Journal of Microbiology

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Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

Nasser Samadi 1 , Iraj Pakzad 1 , 2 , Alireza Monadi Sefidan 3 , Hasan Hosainzadegan 4 , * and Asghar Tanomand 4
Authors Information
1 Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran
2 Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR Iran
3 Department of Microbiology, Tehran University of Medical Sciences, Tehran, IR Iran
4 Department of Basic Sciences, Faculty Of Medicine, Maragheh University of Medical Science, Maragheh , IR Iran
Article information
  • Jundishapur Journal of Microbiology: April 01, 2015, 8 (4); e18102
  • Published Online: April 18, 2015
  • Article Type: Research Article
  • Received: March 9, 2014
  • Revised: April 26, 2014
  • Accepted: May 11, 2014
  • DOI: 10.5812/jjm.18102

To Cite: Samadi N, Pakzad I, Monadi Sefidan A, Hosainzadegan H, Tanomand A. Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran, Jundishapur J Microbiol. 2015 ; 8(4):e18102. doi: 10.5812/jjm.18102.

Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Mingeot-Leclercq MP, Glupczynski Y, Tulkens PM. Aminoglycosides: activity and resistance. Antimicrob Agents Chemother . 1999; 43(4): 727-37
  • 2. Kotra LP, Haddad J, Mobashery S. Aminoglycosides: perspectives on mechanisms of action and resistance and strategies to counter resistance. Antimicrob Agents Chemother. 2000; 44(12): 3249-56[PubMed]
  • 3. Fluit AC, Visser MR, Schmitz FJ. Molecular detection of antimicrobial resistance. Clin Microbiol Rev. 2001; 14(4): 836-71[DOI][PubMed]
  • 4. Shaw KJ, Rather PN, Hare RS, Miller GH. Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol Rev. 1993; 57(1): 138-63[PubMed]
  • 5. Yamane K, Wachino J, Doi Y, Kurokawa H, Arakawa Y. Global spread of multiple aminoglycoside resistance genes. Emerg Infect Dis. 2005; 11(6): 951-3[DOI][PubMed]
  • 6. Toth M, Frase H, Chow JW, Smith C, Vakulenko SB. Mutant APH(2'')-IIa enzymes with increased activity against amikacin and isepamicin. Antimicrob Agents Chemother. 2010; 54(4): 1590-5[DOI][PubMed]
  • 7. Poole K. Aminoglycoside resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2005; 49(2): 479-87[DOI][PubMed]
  • 8. Yu FY, Yao D, Pan JY, Chen C, Qin ZQ, Parsons C, et al. High prevalence of plasmid-mediated 16S rRNA methylase gene rmtB among Escherichia coli clinical isolates from a Chinese teaching hospital. BMC Infect Dis. 2010; 10: 184[DOI][PubMed]
  • 9. Vakulenko SB, Mobashery S. Versatility of aminoglycosides and prospects for their future. Clin Microbiol Rev. 2003; 16(3): 430-50[PubMed]
  • 10. Hopkins KL, Escudero JA, Hidalgo L, Gonzalez-Zorn B. 16S rRNA methyltransferase RmtC in Salmonella enterica serovar Virchow. Emerg Infect Dis. 2010; 16(4): 712-5[DOI][PubMed]
  • 11. Bogaerts P, Galimand M, Bauraing C, Deplano A, Vanhoof R, De Mendonca R, et al. Emergence of ArmA and RmtB aminoglycoside resistance 16S rRNA methylases in Belgium. J Antimicrob Chemother. 2007; 59(3): 459-64[DOI][PubMed]
  • 12. Kacmaz B, Aksoy A. Antimicrobial resistance of enterococci in Turkey. Int J Antimicrob Agents. 2005; 25(6): 535-8[DOI][PubMed]
  • 13. Folster JP, Rickert R, Barzilay EJ, Whichard JM. Identification of the aminoglycoside resistance determinants armA and rmtC among non-Typhi Salmonella isolates from humans in the United States. Antimicrob Agents Chemother. 2009; 53(10): 4563-4[DOI][PubMed]
  • 14. Feizabadi MM, Maleknejad P, Asgharzadeh A, Asadi S, Shokrzadeh L, Sayadi S. Prevalence of aminoglycoside-modifying enzymes genes among isolates of Enterococcus faecalis and Enterococcus faecium in Iran. Microb Drug Resist. 2006; 12(4): 265-8[DOI][PubMed]
  • 15. Saifi M, Pourshafie MR, Eshraghian MR, Soltan Dallal MM. Anti-microbial resistance of Enterococci isolated from urinary tract infections in Iran. Iran Biomed J. 2008; 12(3): 185-90[PubMed]
  • 16. Moniri R, Ghasemi A, Moosavi SGA, Dastehgoli K, Rezaei M, Soares RO, et al. Virulence Gene’s Relationship With Biofilm Formation and Detection of aac (6’)/aph (2”) in Enterococcus faecalis Isolated From Patients With Urinary Tract Infection. Jundishapur J Microbiol . 2013; 6(5)[DOI]
  • 17. Zhang Y, Zhou H, Shen XQ, Shen P, Yu YS, Li LJ. Plasmid-borne armA methylase gene, together with blaCTX-M-15 and blaTEM-1, in a Klebsiella oxytoca isolate from China. J Med Microbiol. 2008; 57: 1273-6[DOI][PubMed]
  • 18. Doi Y, Wachino J, Yamane K, Shibata N, Yagi T, Shibayama K, et al. Spread of novel aminoglycoside resistance gene aac(6')-Iad among Acinetobacter clinical isolates in Japan. Antimicrob Agents Chemother. 2004; 48(6): 2075-80[DOI][PubMed]
  • 19. Ma L, Lin CJ, Chen JH, Fung CP, Chang FY, Lai YK, et al. Widespread dissemination of aminoglycoside resistance genes armA and rmtB in Klebsiella pneumoniae isolates in Taiwan producing CTX-M-type extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 2009; 53(1): 104-11[DOI][PubMed]
  • 20. Yu F, Wang L, Pan J, Yao D, Chen C, Zhu T, et al. Prevalence of 16S rRNA methylase genes in Klebsiella pneumoniae isolates from a Chinese teaching hospital: coexistence of rmtB and armA genes in the same isolate. Diagn Microbiol Infect Dis. 2009; 64(1): 57-63[DOI][PubMed]
  • 21. Yu F, Chen Q, Yu X, Pan J, Li Q, Yang L, et al. High prevalence of plasmid-mediated quinolone resistance determinant aac(6')-Ib-cr amongst Salmonella enterica serotype Typhimurium isolates from hospitalised paediatric patients with diarrhoea in China. Int J Antimicrob Agents. 2011; 37(2): 152-5[DOI][PubMed]
  • 22. Savic M, Lovric J, Tomic TI, Vasiljevic B, Conn GL. Determination of the target nucleosides for members of two families of 16S rRNA methyltransferases that confer resistance to partially overlapping groups of aminoglycoside antibiotics. Nucleic Acids Res. 2009; 37(16): 5420-31[DOI][PubMed]
  • 23. Yamane K, Wachino J, Suzuki S, Shibata N, Kato H, Shibayama K, et al. 16S rRNA methylase-producing, gram-negative pathogens, Japan. Emerg Infect Dis. 2007; 13(4): 642-6[DOI][PubMed]
  • 24. Radhouani H, Poeta P, Goncalves A, Pacheco R, Sargo R, Igrejas G. Wild birds as biological indicators of environmental pollution: antimicrobial resistance patterns of Escherichia coli and enterococci isolated from common buzzards (Buteo buteo). J Med Microbiol. 2012; 61: 837-43[DOI][PubMed]
  • 25. Ho PL, Wong RC, Lo SW, Chow KH, Wong SS, Que TL. Genetic identity of aminoglycoside-resistance genes in Escherichia coli isolates from human and animal sources. J Med Microbiol. 2010; 59: 702-7[DOI][PubMed]
  • 26. Fracalanzza SA, Scheidegger EM, Santos PF, Leite PC, Teixeira LM. Antimicrobial resistance profiles of enterococci isolated from poultry meat and pasteurized milk in Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz. 2007; 102(7): 853-9[PubMed]
  • 27. Jackson CR, Fedorka-Cray PJ, Barrett JB, Ladely SR. Genetic relatedness of high-level aminoglycoside-resistant enterococci isolated from poultry carcasses. Avian Dis. 2004; 48(1): 100-7[PubMed]
  • 28. Salem-Bekhit MM, Moussa IMI, Muharram MM, Elsherbini AM, AlRejaie S. Increasing prevalence of high-level gentamicin resistant enterococci: An emerging clinical problem. Afr J Microbiol Res. 2011; 5(31): 5713-20[DOI]
  • 29. Mohammadi F, Tabaraie B, Sadeghifard N, Ghafoorian S, Maleki A, Davoodian E. Evaluation of drug resistance frequency among Entrococci faecium and E. faecalis strains and detection of VanA/B genes in vancomycin resistance isolated by PCR method in ilam and kermanshah hospitals. Sci J Ilam Uni Med Sci. 2011; 19(2): 1-7
  • 30. Vaziri F, Peerayeh SN, Nejad QB, Farhadian A. The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in Pseudomonas aeruginosa. Clinics (Sao Paulo). 2011; 66(9): 1519-22[PubMed]
  • 31. Wachino J, Arakawa Y. Exogenously acquired 16S rRNA methyltransferases found in aminoglycoside-resistant pathogenic Gram-negative bacteria: an update. Drug Resist Updat. 2012; 15(3): 133-48[DOI][PubMed]
  • 32. Xiao Y, Hu Y. The major aminoglycoside-modifying enzyme AAC(3)-II found in Escherichia coli determines a significant disparity in its resistance to gentamicin and amikacin in China. Microb Drug Resist. 2012; 18(1): 42-6[DOI][PubMed]
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